Skip to main content
SpringerLink
Log in

Zellhybride: Heterokarien, Minizellen, Mikrozellen; Kerntransplantationen

  • Chapter
Book cover Molekular- und Zellbiologie

  • 243 Accesses

Zusammenfassung

1838, im Jahre der Begründung der Zelltheorie durch M. Schieiden, beobachtete J. Müller in Tumorgeweben mehrkernige Zellen, und 1858 fand sie Virchow in einer Reihe pathologischer und normaler Gewebe. Mehrkernigkeit kann auf zweierlei voneinander verschiedenen Ursachen beruhen:

  1. 1.

    Kernteilung ohne gleichzeitige Zellteilung. Hierfür gibt es viele Beispiele. So entsteht z.B. das Plasmodium des Schleimpilzes Physarum auf diese Weise (s. Abb. 53.1). Mehrkernigkeit finden wir auch während der frühen Stadien der Insekteneientwicklung (s. Kap. 62).

  2. 2.

    Fusion durch Verschmelzung mehrerer Zellen, so u.a. bei der Entstehung von Muskelfasern aus Myoblasten (s. Abb. 65.2). Bleiben die Kerne voneinander getrennt, spricht man von Syn- resp. Heterokarien. Verschmelzen sie nach der Zellfusion, spricht man von (Zell-)Hybriden. Das bekannteste Beispiel hierfür ist die Bildung der Zygote nach der Gametenverschmelzung.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 44.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 59.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  • Appels, R., Bolund, L., Ringertz, N.R.: Biochemical analysis of reactivated chick erythrocyte nuclei isolated from chick/HeLa heterokaryons. J. Mol. Biol. 87, 339(1974)

    Article  PubMed  CAS  Google Scholar 

  • Bolund, L., Ringertz, N.R., Harris, H.: Changes in the cytochemical properties of erythrocyte nuclei reactivated by cell fusion. J. Cell Sci. 4, 71 (1969)

    PubMed  CAS  Google Scholar 

  • Bromhall, J.D.: Nuclear transplantation in the rabbit egg. Nature (London) 258, 719 (1975)

    Article  CAS  Google Scholar 

  • Carlsson, S.-A., Luger, O., Ringertz, N.R., Savage, R.E.: Phenotypic expression in chick erythrocyte x rat myoblast hybrids and in chick myoblast x rat myoblast hybrids. Exp. Cell Res. 84, 47 (1974)

    Article  CAS  Google Scholar 

  • Gurdon, J.B.: Molecular biology in a living cell. Nature (London) 248, 772 (1974)

    Article  CAS  Google Scholar 

  • Gurdon, J.B.: Egg cytoplasm and gene control in development. Proc. R.Soc.Lond.B 198, 211 (1977)

    Article  PubMed  CAS  Google Scholar 

  • Gurdon, J.B., Wyllie, A.H., Robertis, E.M. de: The transcription and translation of DNA injected into oocytes. Proc. R. Soc. Lond. B. 283, 367 (1978)

    CAS  Google Scholar 

  • Harris, H., Watkins, J.F.: Hybrid cells derived from mouse and man: Artificial heterokaryons of mammalian cells from different species. Nature (London) 205, 640 (1965)

    Article  CAS  Google Scholar 

  • Harris, H., Watkins, J.F., Ford, C.E., Schoefl, G.I.: Artificial heterokaryons of animal cells from different species. J. Cell Sci. 1, 1 (1966)

    PubMed  CAS  Google Scholar 

  • Harris, H., Sidebottom, E., Grace, D.M., Bramwell, M.E.: The expression of genetic information: A study with hybrid animal cells. J. Cell Sci. 4, 499 (1969)

    PubMed  CAS  Google Scholar 

  • Jonasson, J., Povey, S., Harris, H.: The analysis of malignancy by cell fusion. J. Cell Sci. 24, 217 (1977)

    PubMed  CAS  Google Scholar 

  • Kressmann, A., Clarkson, S.G., Pirrotta, V., Birnstiel, M.L.: Transcription of cloned tRNA gene fragments and subfragments injected into the oocyte nucleus of Xenopus laevis. Proc. Natl. Acad. Sci. USA 75, 1176(1978)

    Article  PubMed  CAS  Google Scholar 

  • Krondahl, U., Bols, N., Ege, T., Linder, S., Ringertz, N.R.: Cells reconstituted from cell fragments of two different species multiply and form colonies. Proc. Natl. Acad. Sci. USA 74, 606 (1977)

    Article  PubMed  CAS  Google Scholar 

  • Mertz, J.E., Gurdon, J.B.: Purified DNAs are transcribed after microinjection into Xenopus oocytes. Proc. Natl. Acad. Sci. USA 74, 1502 (1977)

    Article  PubMed  CAS  Google Scholar 

  • Okada Y.: Analysis of giant polynuclear cell formation caused by HVJ virus from Ehrlich’s ascites tumor cells: Microscopic observation of giant polynuclear cell formation. Exp. Cell Res. 26, 98 (1962)

    Article  PubMed  CAS  Google Scholar 

  • Rapp, F., Westmoreland, D.: Cell transformation by DNA-containing viruses. Biochim. Biophys. Acta 458, 167 (1976)

    PubMed  CAS  Google Scholar 

  • Ringertz, N.R., Savage, R.E.: Cell hybrids. New York, San Francisco, London: Academic Press 1976

    Google Scholar 

  • Ringertz, N.R., Krondahl, U., Coleman, J.R.: Reconstitution of cells by fusion of cell fragments. Exp. Cell Res. 113, 233(1978)

    Article  PubMed  CAS  Google Scholar 

  • Robertis, E.M. de, Gurdon, J.D.: Gene activation in somatic nuclei after injection into amphibian oocytes. Proc.Natl. Acad. Sci. USA 74, 2470 (1977)

    Article  PubMed  Google Scholar 

  • Wiener, F., Klein, G., Harris, H.: The analysis of malignancy by cell fusion: Further evidence of the ability of normal diploid cells to suppress malignancy. J. Cell Sci. 15, 177 (1974a)

    PubMed  CAS  Google Scholar 

  • Wiener, F., Klein, G., Harris, H.: The analysis of malignancy by cell fusion: Hybrids between different tumor cells. J. Cell Sci. 16, 189 (1974b)

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Fakultät für Biologie, Universität Bielefeld, D-4800, Bielefeld 1, Deutschland

    Professor Dr. Peter von Sengbusch

Authors
  1. Professor Dr. Peter von Sengbusch
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 1979 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

von Sengbusch, P. (1979). Zellhybride: Heterokarien, Minizellen, Mikrozellen; Kerntransplantationen. In: Molekular- und Zellbiologie. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67358-0_53

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-67358-0_53

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-67359-7

  • Online ISBN: 978-3-642-67358-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation