Advertisement

Enzyme, die Nukleinsäuren abbauen

  • Peter von Sengbusch

Zusammenfassung

Nukleasen degradieren Nukleinsäuren, wobei Phos-phodiesterbindungen gespalten werden. Man unterscheidet zwischen Ribonukleasen und Desoxyribonu-kleasen, Endo- und Exonukleasen. Die Spaltung kann auf zweierlei Weise erfolgen, einmal entsteht ein Produkt, das am 5′-Ende, im anderen Fall eines, das am 3′-Ende phosphoryliert ist.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  1. Arber, W.: Host controlled variation. In: The bacteriophage lambda. Hershey, A.D. (ed.). New York: Cold Spring Harbor Laboratory 1971Google Scholar
  2. Arber, W., Dussiox, D.: Host specificity of DNA produced in Escherichia coli Host controlled modification of bacteriophage lambda. J. Mol. Biol. 5, 18 (1962)PubMedCrossRefGoogle Scholar
  3. Bigger, C.H., Murray, K., Murray, N.E.: Recognition sequence of a restriction enzyme. Nature New Biol. 244, 7 (1973)PubMedGoogle Scholar
  4. Danna, K., Nathans, D.: Specific cleavage of simian virus 40 DNA by restriction endonuclease of Haemophilus influenzae. Proc. Natl. Acad. Sci. USA 68, 2913(1971)PubMedCrossRefGoogle Scholar
  5. Kelly, TJ., Smith, H.O.: A restriction enzyme from Haemophilus influenzae. Base sequence of the recognition site. J. Mol. Biol. 51, 393 (1970)PubMedCrossRefGoogle Scholar
  6. Luria, S.E.: Host induced modifications of viruses. Cold Spring Harbor Symp. Quant. Biol. 18, 237 (1953)PubMedGoogle Scholar
  7. Meselson, M., Yuan, R.: DNA restriction enzyme from E. coli Nature (London) 217, 1110 (1968)CrossRefGoogle Scholar
  8. Nathans, D., Smith, H.O.: Restriction endonucleases in the analysis and restructuring of DNA molecules. Annu. Rev. Biochem. 44, 273 (1975)PubMedCrossRefGoogle Scholar
  9. Polisky, B., Greene, P., Garfin, D.E., McCarthy, B J., Goodman, H.M., Boyer, H.W.: Specificity of substrate recognition by the EcoR I restriction endonuclease. Proc. Natl. Acad. Sci. USA 72, 3310 (1975)PubMedCrossRefGoogle Scholar
  10. Roberts, R.J., Murray, K.: Restriction endonucleases. CRC Crit. Rev. Biochem, S. 123, Nov. 1976Google Scholar
  11. Smith, H.O., Wilcox, K.W.: A restriction enzyme from Haemophilus influenzae. Purification and general properties. J. Mol. Biol. 51, 379 (1970)PubMedCrossRefGoogle Scholar
  12. Stark, B.C., Kole, R., Bowman, E.J., Altman, S.: Ribonuclease P: An enzyme with an essential RNA component. Proc. Natl. Acad. Sci. USA 75, 3717 (1978)PubMedCrossRefGoogle Scholar
  13. Young, R.A., Argetsinger Steitz, J.: Complementary sequences 1700 nucleotides apart form a ribonuclease III cleavage site in Escherichia coli ribosomal precursor RNA. Proc. Natl. Acad. Sci. USA 75, 3593 (1978)PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • Peter von Sengbusch
    • 1
  1. 1.Fakultät für BiologieUniversität BielefeldBielefeld 1Deutschland

Personalised recommendations