Total and Viable Cell Counts

  • Alois Nowotny


It is essential for almost all experiments involving cells to count the exact cell number per milliliter suspension. In addition, it is frequently necessary to determine the number of viable cells in a cell suspension. Various procedures are available for this purpose. One of them, described here, requires a hemocyto-meter and a simple microscope.


Trypan Blue Pasteur Pipette Fluorescein Diacetate Trypan Blue Exclusion Test Trypan Blue Solution 
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  2. Bodmer, W., Tripp, M. Bodmer, J. See also Transplantion 10 135 (1970)CrossRefGoogle Scholar
  3. Harris, S., Harris, T.N., Farber, M.: J. Immunol. 72, 148 (1954)Google Scholar
  4. Riggs, J.L., Sewiwald, R.J., Burckhalter, J., Downs, C.M., Metcalf, T.J.: Am. J. Pathol. 34, 1081 (1958)PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • Alois Nowotny
    • 1
  1. 1.University of PennsylvaniaPhiladelphiaUSA

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