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Immunoelectrophoresis

  • Alois Nowotny

Abstract

Immunoelectrophoresis, developed by Williams and Grabar (1955), has a much better resolution than gel diffusion. In this system the components of the antigen will first be separated by electrophoresis. The antigen components will move through the agar gel to a distance characteristic for the component in the given system. After the antigen has been separated into its components, antiserum will be put into a channel cut parallel to the direction of the electrophoresis. From this channel, the antibodies will diffuse towards the electrophoretically separated antigen components, and vice versa. Specific precipitate will be formed where an optimal ratio between the corresponding antigen and antibody components has been reached.

Keywords

Paper Strip Veronal Buffer Filter Paper Strip Electrophoresis Chamber Monospecific Antiserum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Crowle, A.J.: Immunodiffusion. New York: Academic Press 1961Google Scholar
  2. Skarnes, R.C.: Ann. N.Y. Acad. Sci. 133, 644 (1966)PubMedCrossRefGoogle Scholar
  3. Uriel, J., Grabar, P.: Bull. Soc. Chim. Biol. 38, 1253 (1956)PubMedGoogle Scholar
  4. Wieme, R.J.: Agar gel electrophoresis. New York, N.Y.: Elsevier 1965Google Scholar
  5. Williams, C.A., Jr., Grabar, P.: J. Immunol. 74, 158 and 397 (1955)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • Alois Nowotny
    • 1
  1. 1.University of PennsylvaniaPhiladelphiaUSA

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