Abstract
Immunoelectrophoresis, developed by Williams and Grabar (1955), has a much better resolution than gel diffusion. In this system the components of the antigen will first be separated by electrophoresis. The antigen components will move through the agar gel to a distance characteristic for the component in the given system. After the antigen has been separated into its components, antiserum will be put into a channel cut parallel to the direction of the electrophoresis. From this channel, the antibodies will diffuse towards the electrophoretically separated antigen components, and vice versa. Specific precipitate will be formed where an optimal ratio between the corresponding antigen and antibody components has been reached.
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References
Crowle, A.J.: Immunodiffusion. New York: Academic Press 1961
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Wieme, R.J.: Agar gel electrophoresis. New York, N.Y.: Elsevier 1965
Williams, C.A., Jr., Grabar, P.: J. Immunol. 74, 158 and 397 (1955)
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© 1979 Springer-Verlag Berlin Heidelberg
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Nowotny, A. (1979). Immunoelectrophoresis. In: Basic Exercises in Immunochemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67356-6_72
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DOI: https://doi.org/10.1007/978-3-642-67356-6_72
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-09453-1
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