Lipid Determination With the Hydroxylamine Method

  • Alois Nowotny


Different classes of lipids which contain ester groups react with alkaline hydroxylamine to form hydroxamic acid. The hydro-xamic acid reacts with acid ferric Perchlorate forming a purple-colored iron chelate complex. Free fatty acids do not react with alkaline hydroxylamine. This procedure was elaborated for quantitative determination of lipids by Snyder and Stephens (1959).


Glycerol Phenol Hydroxyl Hydroxide Hexane 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Snyder, F., Stephens, N.: Biochim. Biophys. Acta 34, 244 (1959)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • Alois Nowotny
    • 1
  1. 1.University of PennsylvaniaPhiladelphiaUSA

Personalised recommendations