Lipid Determination With the Hydroxylamine Method
Different classes of lipids which contain ester groups react with alkaline hydroxylamine to form hydroxamic acid. The hydro-xamic acid reacts with acid ferric Perchlorate forming a purple-colored iron chelate complex. Free fatty acids do not react with alkaline hydroxylamine. This procedure was elaborated for quantitative determination of lipids by Snyder and Stephens (1959).
KeywordsHydroxamic Acid Acid Anhydride Hydroxylamine Hydrochloride Cold Ethanol Pyromellitic Dianhydride
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- Snyder, F., Stephens, N.: Biochim. Biophys. Acta 34, 244 (1959)Google Scholar