Ultramicrodetermination of Reducing Carbohydrates
One of the most sensitive methods for the determination of reducing carbohydrates was elaborated by Park and Johnson (1949). Reducing mono- and oligosaccharides will form potassium ferro-cyanide with potassium ferricyanide in alkaline solution. The addition of ferric ammonium sulfate in a detergent such as sodium lauryl sulfate (or Duponol) will form a colloidal dispersion of ferric-ferrocyanide. The intense blue color of this compound can be measured quantitatively on a spectrophotometer.
KeywordsBoiling Water Bath Sodium Lauryl Sulfate Gluconic Acid Potassium Ferricyanide Amino Sugar
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