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Ultramicrodetermination of Reducing Carbohydrates

  • Alois Nowotny

Abstract

One of the most sensitive methods for the determination of reducing carbohydrates was elaborated by Park and Johnson (1949). Reducing mono- and oligosaccharides will form potassium ferro-cyanide with potassium ferricyanide in alkaline solution. The addition of ferric ammonium sulfate in a detergent such as sodium lauryl sulfate (or Duponol) will form a colloidal dispersion of ferric-ferrocyanide. The intense blue color of this compound can be measured quantitatively on a spectrophotometer.

Keywords

Boiling Water Bath Sodium Lauryl Sulfate Gluconic Acid Potassium Ferricyanide Amino Sugar 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Reference

  1. Park, J.T., Johnson, M.J.: J. Biol. Chem. 181, 149 (1949)PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • Alois Nowotny
    • 1
  1. 1.University of PennsylvaniaPhiladelphiaUSA

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