Ultramicrodetermination of Reducing Carbohydrates

  • Alois Nowotny


One of the most sensitive methods for the determination of reducing carbohydrates was elaborated by Park and Johnson (1949). Reducing mono- and oligosaccharides will form potassium ferro-cyanide with potassium ferricyanide in alkaline solution. The addition of ferric ammonium sulfate in a detergent such as sodium lauryl sulfate (or Duponol) will form a colloidal dispersion of ferric-ferrocyanide. The intense blue color of this compound can be measured quantitatively on a spectrophotometer.


Boiling Water Bath Sodium Lauryl Sulfate Gluconic Acid Potassium Ferricyanide Amino Sugar 
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  1. Park, J.T., Johnson, M.J.: J. Biol. Chem. 181, 149 (1949)PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • Alois Nowotny
    • 1
  1. 1.University of PennsylvaniaPhiladelphiaUSA

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