Determination of Optimal Hydrolytic Conditions
During hydrolysis, not only degradation of large molecules, but also destruction of liberated units such as monosaccharides or amino acids takes place. Both the liberation and destruction of these components depend upon the strength of the hydrolysis, i.e. the acid or alkali used, its concentration, temperature, and the length of the reaction. Obviously, 100% recovery of the liberated components in the hydrolysate is possible only if the hydrolysis is complete, while liberated units are fully resistant to further degradation. No such hydrolytic procedure exists. Therefore, quantitative determination of the monosaccharide or amino acid constituents of a biopolymer always results in a “minimum value” which is significantly less than the actual percentage.
KeywordsAmino Sugar Cold Finger Amino Acid Constituent Hydrolytic Procedure Optimal Hydrolysis
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- Kasai, N., Nowotny, A.: J. Bacterol. 94, 1824 (1967)Google Scholar