Preparation of 14C-Labeled Endotoxin and Endotoxic Glycolipid (EGL)

  • Alois Nowotny

Abstract

LPS can be labeled by 51Cr which forms an undefined complex with the toxic components of the structure (Braude et al., 1955). Such compounds were used extensively in studying the fate of 51Cr-LPS, under normal as well as pathologic conditions. A shortcoming of this otherwise excellent procedure is that the 51Cr label is detected by it with or without the carrier LPS molecule, which means that the presence of the label not necessarily indicates the presence of the entire complex. The procedure des-, cribed here was used in our laboratory by Dr. Chen-lo H. Chen and it applies covalently bound 14C which is incorporated into the entire bacterial cell through biosynthesis. Extraction and purification of the labeled endotoxic LPS or EGL is carried out as described in Exercise 21.

Keywords

Fermentation Magnesium Phenol Agar Chloroform 

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References

  1. Baserga, R.: Methods in Cancer Research 1, 45 (1967)Google Scholar
  2. Braude, A.I., Carey, F.J., Sutherland, D., Zalesky, M.J.: Clin. Invest. 34, 850 (1955)CrossRefGoogle Scholar
  3. Chen, C.H., Johnson, A.G., Kasai, N., Key, B.A., Levin, J., Nowotny, A.: J. Infec. Dis. 128S, 43 (1973)CrossRefGoogle Scholar
  4. Nowotny, A., Cundy, K.R., Neale, N.L., Nowotny, A.M., Radvany, R., Thomas, S.P., Tripodi, D.J.: Ann. N. Y. Acad. Sci. 133, 586 (1966)PubMedCrossRefGoogle Scholar
  5. Ulevitch, R.J.: Immunochem. 15, 157 (1978)CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • Alois Nowotny
    • 1
  1. 1.University of PennsylvaniaPhiladelphiaUSA

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