Abstract
Extraction of O-antigens (or endotoxins) from bacterial cells was first described by Boivin, Mesrobeanu and Mesrobeanu (1933). The trichloroacetic acid extraction procedure used by these workers dissolves a peptide-containing glycolipid from the cell walls. In some cases, depending on the bacterial strain used, the TCA extract also contains nucleic acids derived from the cells. Numerous other procedures for the isolation of endotoxic O-antigens from bacteria have also been published. This Exercise in Part A presents a slightly modified version of the original Boivin, Mesrobeanu and Mesrobeanu procedure.
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References
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© 1979 Springer-Verlag Berlin Heidelberg
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Nowotny, A. (1979). Extraction of Bacterial O Antigens (Endotoxins or lipopolysaccharides). In: Basic Exercises in Immunochemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67356-6_21
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DOI: https://doi.org/10.1007/978-3-642-67356-6_21
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