Advertisement

Collection, Cryopreservation and Transplantation of Blood Stem Cells in the Treatment of Hemopoietic Failure

  • T. M. Fliedner
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 25)

Abstract

It is the purpose of this paper to review the experimental studies which have been carried out in our group to explore the possibilities and limitations to treat hemopoietic failure induced by whole body x-irradiation by means of blood derived hemopoietic stem cells under autologous and allogeneic conditions. Some of the results have been published or are accepted for publication [1, 2, 5–17, 21]. The findings can be summarized in 5 statements supported by sufficient experimental evidence.

Keywords

Blood Stem Cell Mononuclear Leukocyte Hemopoietic Stem Cell Bone Marrow Recovery Blood Derive Stem Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Calvo, W., Fliedner, T. M., Herbst, E., Hügl, E., Bruch, C.: Regeneration of blood forming organs after autologous leukocyte transfusion in lethally irradiated dogs. II. Distribution and cellularity of the marrow in irradiated and transfused animals. Blood 47, 593–601 (1976)PubMedGoogle Scholar
  2. 2.
    Calvo, W., Fliedner, T. M., Steinbach, I., Alcober, V., Nothdurft, W., Fache, I.: Morphologic alterations in canine marrow of long-term survivors after 1200 R whole-body x-irradiation and autologous blood leukocyte engraftment. Am J. Pathol. 95, 379–388 (1979)PubMedGoogle Scholar
  3. 3.
    Dicke, K. A., Bekkum, D. W. van: Avoidance of acute secondary disease by purification of hemopoietic stem cells with density gradient centrifugation. Exp. Hematol. 20, 126–130 (1970)Google Scholar
  4. 4.
    Doan, C. A.: On the origin and developmental potientialities of blood cells. Bull. NY Acad. Med. 15, 668–697 (1939)Google Scholar
  5. 5.
    Fliedner, T. M., Flad, H.-D., Bruch, C., Calvo, W., Goldmann, S., Herbst, E., Hügl, E., Huget, R., Körbling, M., Krumbacher, K., Nothdurft, W., Ross, W. M., Schnappauf, H.-P., Steinbach, I.: Treatment of aplastic anemia by blood stem cell transfusion: a canine model. Haematologica 61, 141–156 (1976)PubMedGoogle Scholar
  6. 6.
    Fliedner, T. M., Körbling, M., Calvo, W., Bruch, C., Herbst, E.: Cryopreservation of blood mononuclear leukocytes and stem cells suspended in a large fluid volume. Blut 35, 195–202 (1977)PubMedCrossRefGoogle Scholar
  7. 7.
    Fliedner, T. M., Calvo, W., Körbling, M., Kreutzmann, H., Nothdurft, W., Ross, W. M., Vassileva, D.: Hematopoietic stem cells in blood: characteristics and potentials. In: Hematopoietic cell differentiation. Golde, D. W. et al. (eds.), pp. 193–212. New York, San Francisco, London: Academic Press 1978.Google Scholar
  8. 8.
    Fliedner, T. M., Calvo, W.: Hematopoietic stem-cell seeding of a cellular matrix: a principle of initiation and regeneration of hematopoiesis. In: Differentiation of normal and neoplastic hematopoietic cells. Cold Spring Harbor Laboratory 1978Google Scholar
  9. 9.
    Fliedner, T. M., Calvo, W., Körbling, M., Nothdurft, W., Pflieger, H., Ross, W. M.: Collection, storage and transfusion of blood stem cells for the treatment of hemopoietic failure. Blood Cells 5, 313–328 (1979)PubMedGoogle Scholar
  10. 10.
    Fliedner, T. M., Körbling, M., Arnold, R., Grilli, G., Haen, M., Kreutzmann, H., Pflieger, H.: Collection and cryopreservation of mononuclear blood leukocytes and CFU-C in man. Exp. Hematol. 7, Suppl. 5, 398–408 (1979)PubMedGoogle Scholar
  11. 11.
    Gerhartz, H. H., Fliedner, T. M.: Grarrulozytär determinierte Stammzellen (CFU-C) aus Knochenmark und Blut: Größenunterschiede vor und nach Dextransulfat-Mobilisation. Blut 38, 161–164 (1979)PubMedCrossRefGoogle Scholar
  12. 12.
    Gerhartz, H. H., Fliedner, T. M.: Velocity sedimentation and cell cycle characteristics of granulopoietic progenitor cells (CFU-C) in canine blood and bone marrow: Influence of mobilization and CFU-C depletion. Exp. Hematol. (accepted for publication)Google Scholar
  13. 13.
    Herbst, W. E., Fliedner, T. M., Calvo, W., Schnappauf, H.-P., Meyer, H.: Untersuchungen über die Gewinnung hämopoetischer Stammzellen aus dem peripheren Blut von Hunden und über ihre Fähigkeit, die Blutzellbildung zu regenerieren. Blut 30, 265–276 (1975)PubMedCrossRefGoogle Scholar
  14. 14.
    Kelemen, E., Calvo, W., Fliedner, T. M.: Atlas of human hemopoietic development. Heidelberg, Berlin, New York: Springer 1979Google Scholar
  15. 15.
    Körbling, M., Fliedner, T. M., Calvo, W., Nothdurft, W., Ross, W. M.: In-vitro and in-vivo properties of canince blood mononuclear leukocytes separated by discontinuous albumin density gradient centrifugation. Biomedicine 26, 275–283 (1977)PubMedGoogle Scholar
  16. 16.
    Körbling, M., Fliedner, T. M., Nothdurft, W., Calvo, W., Ross, W. M., Steinbach, I.: Albumin density purification of canine hemopoietic blood stem cells (HBSC): Long-term allogeneic engraftment without gvh-reaction. Exp. Hematol. accepted for publication (1979)Google Scholar
  17. 17.
    Kovács, P., Bruch, C., Herbst, E. W., Fliedner, T. M.: Collection of in vitro colony-forming units from dogs by repeated continuous flow leukaphereses. Acta Haematol. 60, 172–181 (1978)PubMedCrossRefGoogle Scholar
  18. 18.
    Metcalf, D., Moore, M. A. S.: Hemopoietic cells. Amsterdam, London: North-Holland Publishing Company 1971Google Scholar
  19. 19.
    Micklem, H. S., Ogden, D. A., Evans, E. P., Ford, C. E., Gray, I. G.: Compartments and cell flows within the mouse haemopoietic system. II. Estimated rates of interchange. Cell Tissue Kinet. 8, 233–248 (1975)PubMedGoogle Scholar
  20. 20.
    Moore, M. A. S., Metcalf, D.: Ontogeny of the hemopoietic system: Yolk sac origin of in-vivo and in-vitro colony forming cells in the developing mouse embryo. Br. J. Haematol. 18, 279–283 (1970)PubMedCrossRefGoogle Scholar
  21. 21.
    Nelson, B., Calvo, W., Fliedner, T. M., Herbst, E., Bruch, C., Schnappauf, H.-P., Flad, H.-D.: The repopulation of lymph nodes of dogs after 1200 R whole-body x-irradiation and intravenous administration of mononuclear blood leukocytes. Am. J. Pathol. 84, 259–278 (1976)PubMedGoogle Scholar
  22. 22.
    Nothdurft, W., Bruch, C., Fliedner, T. M., Rüber, E.: Studies on the regeneration of the CFU-C population in blood and bone marrow of lethally irradiated dogs after autologous transfusion of cryopreserved mononuclear blood cells. Scand. J. Haematol. 19, 470–481 (1977)PubMedCrossRefGoogle Scholar
  23. 23.
    Nothdurft, W., Fliedner, T. M., Calvo, W., Flad, H.-D., Huget, R., Körbling, M., Krumbacher von Loringhofen, K., Ross, W. M., Schnappauf, H.-P., Steinbach, I.: CFU-C populations in blood and bone marrow of dogs after lethal irradiation and allogeneic transfusion with cryopreserved blood mononuclear cells. Scand. J. Haematol. 21, 115–130 (1978)PubMedCrossRefGoogle Scholar
  24. 24.
    Nothdurft, W., et al.: Unpublished observationsGoogle Scholar
  25. 25.
    Rohr, K.: Das menschliche Knochenmark. Stuttgart: Thieme 1960Google Scholar
  26. 26.
    Ross, W. M., Körbling, M., Nothdurft, W., Calvo, W., Fliedner, T. M.: Characterization of bone marrow and lymph node repopulating cells by transplanting mononuclear cells into radiated dogs. In: Experimental hematology today. Baum, S. J., Ledney, G. D. (eds.) pp. 29–38. New York: Springer 1977Google Scholar
  27. 27.
    Ross, W. M., Körbling, M., Nothdurft, W., Fliedner, T. M.: The role of dextran sulfate in increasing the CFU-C concentration in dog blood. Proc. Soc. Exp. Biol. Med. 157, 301–305 (1978)PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • T. M. Fliedner

There are no affiliations available

Personalised recommendations