Summary
Graft-versus-host reactions in mice can be suppressed by incubation of the donor cells with T-cell-specific antibodies prior to transplanting them into lethally irradiated recipients. Since reconstitution of the immune functions in these recipients requires the formation of a new T-lymphocyte population, we investigated whether H-2 differences between recipient thymus and donor stem cells as well as an age dependent thymic involution impaired the maturation of immunocompetent T-lymphocytes. For this purpose the immune response of different chimeric mice against SRBC and third party skin grafts as well as the repopulation of the lymphatic organs with T-lymphocytes was evaluated. Chimeras of the type CBL → CBL/CBA and CBL/CBA → CBA were used for the experiments. In addition chimeras which had been thymectomized, transplanted with the thymus of one parent strain, lethally irradiated and transfused with bone marrow from the other parent strain were analyzed. In all chimeric combinations, even in the case of complete H-2 difference between donor cells and recipient thymus, a normal or almost normal reconstitution of the immune response against SRBC and incompatible skin grafts was detected. Furthermore age-involuted thymi returned to normal morphologically and functionally after bone marrow transplantation. The repopulation of the lymphatic organs in the investigated chimeric mice was remarkably similar to normal controls. This indicates that hemopoietic stem cells and/or lymphopoietic progenitors may differentiate in a thymus in spite of H-2 differences to functional T-lymphocytes able to respond against SRBC and incompatible skin grafts.
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References
Dresser, D.W., Wortis, H. H.: Use of antiglobulin serum to detect cells producing antibody with low hemolytic efficiency. Nature 208, 859–861 (1965)
East, J., Parrott, D.: Prevention of wasting in mice thymectomized at birth and their subsequent rejection of allogeneic leukemic grafts. J. Nat. Cancer Inst. 33, 673 (1964)
Hirokawa, K., Makinodan, T.: Thymic involution: Effect on T-cell differentiation. J. Immunol. 114, 1659–1664 (1975)
Hoffmann-Fezer, G., Rodt, H., Eulitz, M., Thierfelder, S.: Immunohistochemical identification of T- and B-lymphocytes delineated by the unlabeled antibody enzyme method. I. Anatomical distribution of D-positive and Ig-positive cells in lymphoid organs of mice. J. Immunol. Meth. 13, 261–270(1976)
Jerne, N. K., Nordin, A. A.: Plaque formation in agar by single antibody-producing cells. Science 140, 405 (1963)
Kindred, B., Loor, F.: Activity of host-derived T-cells which differentiate in nude mice grafted with co-isogeneic or allogeneic thymuses. J. Exp. Med. 139, 1215–1227 (1974)
Kolb, H. J., Rieder, I., Rodt, H., Netzel, B., Grosse-Wilde, H., Scholz, S., Schäffer, E., Kolb, H., Thierfelder, S.: Antilymphocytic antibody and marrow transplantation VI. Graft-versus-host tolerance in DLA-incompatible dogs following “in vitro” treatment of bone marrow with absorbed antithymocyte globulin. Transplantation (in press)
Loughman, B. E., Nordin, A. A., Bealmar, P. M.: Studies of the immunological capacity of germ-free mouse radiation chimeras. I. Chimerism and humoral immune response. Cell. Immunol. 9, 104–117 (1973)
Lydyard, P. M., Ivanyi, J.: Chimerism of immunocompetent cells in allogeneic bone marrow-reconstituted lethally irradiated chickens. Transplantation 20, 155–162 (1975)
Rodt, H.: Herstellung und Spezifität heterologer Anti-T-Lymphozyten-Seren und ihre Anwendung im Rahmen der Knochenmarktransplantation und der Leukämiediagnostik. GSF Bericht H 604, München 1979
Rodt, H., Thierfelder, S., Eulitz, M.: Suppression of acute secondary disease by heterologous anti-brain-serum. Blut 25, 385–389 (1972)
Rodt, H., Thierfelder, S., Eulitz, M.: Antilymphoçytic antibodies and marrow transplantation. III. Effect of heterologous anti-brain antibodies on acute secondary disease in mice. Eur. J. Immunol. 4, 25–29 (1974)
Rodt, H., Kolb, H. J., Netzel, B., Rieder, I., Janka, G., Belohradsky, B.,Haas, R. J., Thierfelder, S.: GVHD suppression by incubation of bone marrow grafts with anti-T-cell globulin: Effect in the canine model and application to clinical bone marrow transplantation. Transpl. Proc. (in press)
Schaefer, H. E., Fischer, R.: Der Peroxydase-Nachweis an Ausstrichpräparaten sowie an Gewebeschnitten nach Entkalkung und Paraffineinbettung. Klin. Wochenschr. 46, 1228–1230 (1968)
Sprent, J., v. Böhmer, H., Nabholz, M.: Association of immunity and tolerance to host H-2 determinants in irradiated Fl-hybrid mice reconstituted with bone marrow cells from one parental strain. J. Exp. Med. 142, 321–331 (1975)
Sternberger, L. A.: Immunocytochemistry, pp. 129–137. Englewood Cliffs, N. J.: Prentice Hall Inc. 1974
Trentin, J. J., Judd, K. P.: Prevention of acute graft-versus-host (GVH) mortality with spleen-absorbed anti-thymocyte globulin (ATG). Transpl. Proc. 5, 865–868 (1973)
Urso, P., Gengozian, N.: T-cell deficiency in mouse allogenic radiation chimeras. J. Immunol. 111, 712–719(1973)
Urso, P., Gengozian, N.: Variation in T- and B-cell deficiency in different mouse allogeneic radiation chimeras. J. Immunol. 113, 1770–1778 (1974)
Zinkernagel, R. M., Callahan, G. N., Althage, A., Cooper, J., Klein, P. A., Klein, J.: On the thymus in the differentiation of “H-2 self-recognition” by T-cells: Evidence for dual recognition. J. Exp. Med. 147, 882–896 (1978)
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Rodt, H., Thierfelder, S., Hoffmann-Fezer, G. (1980). Influence of the Recipient Thymus on the Maturation of T-Lymphocytes in H-2 Different Radiation Chimeras. In: Thierfelder, S., Rodt, H., Kolb, H.J. (eds) Immunobiology of Bone Marrow Transplantation. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 25. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67319-1_16
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DOI: https://doi.org/10.1007/978-3-642-67319-1_16
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