The Chromosome Distribution of Homologous Sequences to the Four Human Satellite DNAs in the Hominidae

  • Héctor N. Seuánez


Techniques of in situ hybridisation, developed by Gall and Pardue (1969), have enabled us to detect and localize repetitive DNA sequences in chromosomes. This method has frequently been applied to study the chromosome distribution of repetitive DNA sequences in man. Each human satellite DNA has been used as a template for in vitro synthesis of a radioactively complementary RNA (cRNA), using a bacterial DNA-dependent RNA polymerase. Each cRNA was then applied to denatured human chromosome preparations, and the sites where stable cRNA-DNA hybrids were formed were subsequently identified with autoradiography. Different amounts of these hybrid molecules, both in vitro and in situ, may be formed according to the temperature of incubation (Moar et al., 1975), because each of the three human satellite DNAs (I, II, and III) proved to have an optimum temperature (T OPT) at which cRNA-DNA hybridisation was most effective. Above their respective T OPJ, the amount of hybridisation detected in vitro and in situ decreased.


Minor Site Centromere Region Satellite DNAs Chromosome Complement Acrocentric Chromosome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Evans, H.J., Gosden, J. R., Mitchell, A.R., Buckland, R.A.: Location of human satellite DNAs on the Y chromosome. Nature (London) 251, 346–347 (1974)CrossRefGoogle Scholar
  2. Gall, J., Pardue, M.L.: Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc. Nat. Acad. Sci. USA 63, 378–383 (1969)PubMedCrossRefGoogle Scholar
  3. Gosden, J.R., Mitchell, A.R., Buckland, R.A., Clayton, R.P., Evans, H. J.: The location of four human satellite DNAs on human chromosomes. Exp. Cell Res. 92, 148–158 (1975)PubMedCrossRefGoogle Scholar
  4. Gosden, J.R., Mitchell, R.A., Seuánez, H.N., Gosden, C.: The distribution of sequences complementary to satellite I, II and IV DNAs in the chromosomes of the chimpanzee (Pan troglodytes), gorilla (Gorilla gorilla), and orangutan (Pongo pygmaeus). Chromosoma (Berlin) 63, 253–271 (1977)CrossRefGoogle Scholar
  5. Jones, K.W., Corneo, G.: Location of satellite and homogeneous DNA sequences on human chromosomes. Nature New Biol. 233, 267–271 (1971)CrossRefGoogle Scholar
  6. Jones, K.W., Prosser, J., Corneo, G., Ginelli, E.: The chromosomal location of human satellite III. Chromosoma (Berlin) 42, 445–45F (1973)CrossRefGoogle Scholar
  7. Jones, K. W., Prosser, J., Corneo, G., Ginelli, E., Bobrow, M.: Satellite DNA constitutive heterochromatin and human evolution. In: Modern aspects of cytogenetics: constitutive heterochromatin in man. Symposia Medica Hoechst. Pfeiffer, R. A. (ed.), Vol, VI, pp. 45–61. Stuttgart, New York 1972Google Scholar
  8. Jones, K.W., Purdom, I.F., Prosser, J., Corneo, G.: The chromosomal localization of human satellite DNA I. Chromosoma (Berlin) 49, 161–171 (1974)CrossRefGoogle Scholar
  9. Melli, M., Ginelli, E., Corneo, G., di Lernia, R.: Clustering of the DNA sequences complementary to repetitive nuclear DNA of HeLa cells. J. Mol. Biol. 93, 23–38 (1975)PubMedCrossRefGoogle Scholar
  10. Mitchell, A. R., Seuánez, H.N., Lawrie, S., Martin, D.E., Gosden, J.R.: The location of DNA homologous to human satellite III DNA in the chromosomes of chimpanzee (Pan troglodytes), gorilla (Gorilla gorilla) and orangutan (Pongo pygmaeus). Chromosoma (Berlin) 61, 345–358 (1977)CrossRefGoogle Scholar
  11. Moar, M. H., Purdom, I.F., Jones, K.W.: Influences of temperature on the detectability and chromosomal distribution of specific DNA sequences by in situ hybridisation. Chromosoma (Berlin) 53, 345–359 (1975)CrossRefGoogle Scholar
  12. Seuánez, H., Mitchell, A., Gosden, J. R.: Constitutive heterochromatin in the Hominidae in relation to four satellite DNAs in man and homologous sequences in the great apes. In: Proc. III Latin-Amer. Congr. Genet, Montevideo, 1977, Joint Seminar and Workshop. Aspects of Chromosome Organization and Function. Drets, M. E., Brum-Zorrilla, N., Folke, G. A. (eds.), 171–178 (1977)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • Héctor N. Seuánez
    • 1
  1. 1.Department of Genetics, Institute of BiologyUniversidade Federal do Rio de JaneiroBrazil

Personalised recommendations