Recombination: Recombinant DNA Research, Techniques and Results
During the last few years, a biological method for the isolation of specific DNA fragments has been developed, and its application is continuously being improved and extended. This technique of DNA cloning makes use of bacteria and bacterial plasmids to segregate and propagate foreign DNA and allows the isolation of almost any gene or DNA fragment which can be identified outside the organism of origin. The in vitro recombination of unselected DNA fragments with a bacterial vector DNA constitutes a crucial step in all DNA cloning procedures, and has played an important role in the development of molecular cloning. The method can be applied to all biological problems studied at the DNA level and with its broad applicability, it can be compared with a technique like molecular hybridization.
KeywordstRNA Gene EcoRI Fragment Bacterial Plasmid Cloning Vehicle Tyrosine tRNA
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