Abstract
During the last few years, a biological method for the isolation of specific DNA fragments has been developed, and its application is continuously being improved and extended. This technique of DNA cloning makes use of bacteria and bacterial plasmids to segregate and propagate foreign DNA and allows the isolation of almost any gene or DNA fragment which can be identified outside the organism of origin. The in vitro recombination of unselected DNA fragments with a bacterial vector DNA constitutes a crucial step in all DNA cloning procedures, and has played an important role in the development of molecular cloning. The method can be applied to all biological problems studied at the DNA level and with its broad applicability, it can be compared with a technique like molecular hybridization.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1978 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Hollenberg, C.P. (1978). Recombination: Recombinant DNA Research, Techniques and Results. In: Ellenberg, H., Esser, K., Merxmüller, H., Schnepf, E., Ziegler, H. (eds) Progress in Botany/Fortschritte der Botanik. Progress in Botany/Fortschritte der Botanik, vol 40. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67120-3_11
Download citation
DOI: https://doi.org/10.1007/978-3-642-67120-3_11
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-67122-7
Online ISBN: 978-3-642-67120-3
eBook Packages: Springer Book Archive