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Recombination: Recombinant DNA Research, Techniques and Results

  • Cornelis P. Hollenberg
Part of the Progress in Botany/Fortschritte der Botanik book series (BOTANY, volume 40)

Abstract

During the last few years, a biological method for the isolation of specific DNA fragments has been developed, and its application is continuously being improved and extended. This technique of DNA cloning makes use of bacteria and bacterial plasmids to segregate and propagate foreign DNA and allows the isolation of almost any gene or DNA fragment which can be identified outside the organism of origin. The in vitro recombination of unselected DNA fragments with a bacterial vector DNA constitutes a crucial step in all DNA cloning procedures, and has played an important role in the development of molecular cloning. The method can be applied to all biological problems studied at the DNA level and with its broad applicability, it can be compared with a technique like molecular hybridization.

Keywords

tRNA Gene EcoRI Fragment Bacterial Plasmid Cloning Vehicle Tyrosine tRNA 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1978

Authors and Affiliations

  • Cornelis P. Hollenberg
    • 1
  1. 1.Abt. BeermannMax-Planck-Institut für BiologieTübingenGermany

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