Cocultivation as a Tool for the Detection of Oncoviruses in Childhood Leukemia
In our study on the possible role of type-C oncoviruses in human leukemia we applied the technique of cocultivation of human bone marrow cells with an animal indicator cell line. The cocultivation technique proved to be very useful, for instance, for the isolation of endogenous primate viruses (Todaro et al., 1978). When bone marrow of a leukemic child was cocultivated with the rat XC cell line, a type C virus was readily detected which was related to the simian sarcoma-virus (SiSV) (Nooter et al., 1975). The use of the XC cell line has been abandoned by us because of the pronounced cytopathic effect of primate viruses in this line and the danger of activation of the endogenous rat virus.
KeywordsBovine Leukemia Virus Childhood Leukemia Endpoint Titer Human Bone Marrow Cell Human Lymphoid Cell
Unable to display preview. Download preview PDF.