Abstract
Biologic and biochemical parameters for detection and judgement of increased occupational lead exposure have been known for several decades. This procedure may meet the need to avoid the still relatively sophisticated blood-lead analysis. On the other hand the determination of such data will hopefully lead to additional information about the extense of the harmful effects of lead on organisms (17). Few of the numerous biochemical parameters still used in the field of occupational medicine to determine an increased lead load have proved successful in routine operation. The methods generally used nowadays are of measuring δ-aminolevulinic acid in urine and δ-aminolevulinic-aciddehydratase activity of erythrocytes. Recent examinations confirm that the measurement of erythrocytic porphyrins (EP) is a specific and sensitive criterion of the influence of lead on man (1, 9, 15, 17, 20, 22). Fast and reliable fluorometric micromethods for the EP determination have been developed in recent years (4, 5, 6, 8, 9, 12, 13, 17).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Alessio, L., Bertazzi, P.A., Toffoletto, F., Foa, A.: Free Erythrocyte Protoporphyrin as an Indicator of the Biological Effect of Lead in Adult Males. Int. Arch. Occup. Environ. Health 37, 73–88 (1976)
Blumberg, W.E., Eisinger, J., Lamola, A.A., Zuckerman, D.M.: The Hematofluorometer. Clin. Chem. 23, 270–274 (1977)
Chisholm, J.J., Jr.: Screening techniques for undue lead exposure in children: Biological and practical considerations. J. Pediatr. 79, 719–725 (1971)
Chisholm, J.J., Jr., Mellits, E.D., Keil, J.E., Barret, M.B.: A simple protoporphyrin assay - Microhematocrit procedure as a screening technique for increased lead absorption in young children. Pediatrics 84, 490–496 (1974)
Chisholm, J.J., Brown, D.H.: Micro-Scale photofluorometric determination of “Free Erythrocyte Porphyrin” (Protoporphyrin IX). Clin. Chem. 21, 1669 (1975)
Fischbein, A., Eisinger, J., Blumberg, W.E., Lilis, R., Selikoff, I.J.: Fluorometric zinc protoprophyrin determination in blood - a practical method for the detection of chronical lead poisoning. Paper presented at the 2nd international workshop on occupational lead exposure. Amsterdam, 21.-23. Sept. 1976
Fischbein, A., Eisinger, J., Blumberg, W.E.: Zinc protoporphyrin determination: A rapid screening test for the detection of lead poisoning. Mt. Sinai J. Med. N.Y. 43, 294–299 (1976)
Granick, S., Sassa, S., Granick, J.L., Levere, R.D., Kappas, A.: Assay for porphyrins, δ-aminolevulinic-acid dehydratase, and porphyrinogen synthetase in microliter samples of whole blood: Applications to metabolic defects involving the heme pathway. Proc. Natl. Acad. Sci. U.S.A. 69, 2381–2385 (1972)
Hanna, Th.L., Dietzler, D.N., Smith, C.H., Gupta, S., Zarkowsky, H.S.: Erythrocyte porphyrin analysis in the detection of lead poisoning in children: Evaluation of four micromethods. Clin. Chem. 22, 161 (1976)
Kammholz, L.P., Thatcher, L.G., Blodgett, F.M., Good, Th.A.: Rapid protoporphyrin quantitation for detection of lead poisoning. Pediatrics 50, 625–631 (1972)
Lamola, A., Joselow, M., Yamane, T.: Zinc protoporphyrin (ZPP): A simple sensitive fluorometric screening test for lead poisoning. Clin. Chem. 21, 93 (1975)
Piomelli, S., Davidow, B., Guinee, V.F., Young, P., Gay, G.: The FEP (free erythrocyte porphyrins) test: A screening micromethod for lead poisoning. Pediatrics 51, 254–259 (1973)
Piomelli, S., Young, P., Gay, G.: A micromethod for free erythrocyte porphyrins: The FEP test. J. Lab. Clin. Med. 81, 932–940 (1973)
Piomelli, S.: Free Erythrocyte Porphyrins in the Detection of Undue Absorption of Pb and of Fe Deficiency. Clin. Chem. 23, 264–269 (1977)
Roels, H.A., Lauwerys, R.R., Buchet, J.P., Vrelust, M.Th.: Response of free erythrocyte porphyrin and urinary 5-aminolevulinic acid in men and women moderately exposed to lead. Int. Arch. Arbeitsmed. 34, 97–108 (1975)
Sassa, S., Granick, J.L., Granick, S., Kappas, A., Levere, R.D.: Studies in lead poisoning. I. Microanalysis of erythrocyte protoporphyrin levels by spectrofluorometry in the detection of chronic lead intoxiation in the subclinical range. Biochem. Med. 8, 135–148 (1973)
Schiele, R., Schaller, K.H., Wagner, H.M.: Die Bestimmung der freien Erythrozyten porphyrine als schneller Suchtest einer erhöhten Bleiexposition und seine Validität im Vergleich zum Blutbleispiegel und zur Delta-Aminolaevulinsäure-Dehydratase-Aktivität. Schr.Reihe Ver. Wass.-Boden-Lufthyg. H. 41, 231-240. Stuttgart: Gustav Fischer Verlag 1974
Schiele, R., Wagner, H.M., Schaller, K.H.: Die Bestimmung der freien Erythrozyten porphyrine als schneller Suchtest einer beruflichen Bleiexposition. In: Bericht über die 15. Jahrestagung der Dtsch. Ges. f. Arbeitsmed., München 24.-26.4.1975. Brenner, W., Rohmert, W., Rutenfranz, J. (eds.). Stuttgart: A.W. Gentner-Verlag 1976a, pp. 133–139
Schiele, R., Schaller, K.H., Valentin, H.: Moderne Kriterien zur Diagnostik und Beurteilung chronischer Bleibelastung in der Arbeitswelt. In: Berufskrankheiten in der keramischen und Glas-Industrie, H. 27, Bad Reichenhaller Kolloquium 1976. Berufsgenossenschaft der keramischen und Glas-Industrie (ed.). Würzburg: 1977, pp. 21-36
Schlegel, H.: Laboratoriumsdiagnostik der chronischen Bleivergiftung. Dtsch. Med. Wochenschr. 95, 2131–2132 (1970)
Schlegel, H., Kufner, G.: Bleivergiftungs-Prävention. Dtsch. Med. Wochenschr. 101, 246–247 (1976)
Schmidt, D., Stich, W.: Diagnostische Kriterien erhöhter Bleiaufnahme. Münch. Med. Wochenschr. 115, 103–108 (1973)
Schwartz, S., Wikoff, H.M.: The relation of erythrocyte coproporphyrin and protoporphyrin to erythropoieses. J. Biol. Chem. 194, 563–573 (1952)
Tomokuni, K., Osaka, J., Ogata, M.: Erythrocyte Protoporphyrin Test for Occupational Lead Exposure. Arch. Environ. Health 30, 588–590 (1975)
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1978 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Schaller, K.H., Schiele, R. (1978). Comparison of Fluorometric Micromethods for Analyzing Erythrocytic Porphyrins (EP). In: Doss, M. (eds) Diagnosis and Therapy of Porphyrias and Lead Intoxication. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67002-2_35
Download citation
DOI: https://doi.org/10.1007/978-3-642-67002-2_35
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-08863-9
Online ISBN: 978-3-642-67002-2
eBook Packages: Springer Book Archive