In Vitro and in Vivo Investigations on Antibody-Dependent Cellular Cytotoxicity

  • Gary R. Pearson
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 80)


It is now well established that target cell destruction can be mediated through the interaction of antibody with normal lymphoid cells as well as through the classic T-cell immunity mechanism (Cerottini and Brunner, 1974). This phenomenon, recently reviewed by Perlmann, Perlmann, and Wigzell (1972), was first described by Möller in 1965 and has received a number of designations, including antibody-dependent cell-mediated (or cellular) cytotoxicity (ADCC), antibody-dependent lymphocyte cytotoxicity (ADLC), cell dependent antibody-mediated cytotoxicity (CDAMC), and lymphocyte-dependent antibody (LDA). For the purpose of this review, this immunologic reaction will be referred to as ADCC.


Effector Cell ADCC Activity Cytotoxic Reaction Normal Human Lymphocyte Murine Sarcoma Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Akira, D., Takasugi, M.: Loss of specific natural cell-mediated cytotoxicity with absorption of natural antibodies from serum. Int. J. Cancer 19, 747–755 (1977)PubMedCrossRefGoogle Scholar
  2. Austin, R., Daniels, C.A.: Inhibition by rheumatoid factor, anti-Fc, and staphylococcal protein A of antibody-dependent cellmediated cytolysis against Herpes simplex virus- infected cells. J. Immunol. 777, 2, 602–607 (1976)Google Scholar
  3. Basten, A., Warner, N.L., Mandel, T.: A receptor for antibody on B lymphocytes. II. Immunochemical and electron microscopy characteristics. J. Exp. Med. 135, 627–642 (1972)PubMedCrossRefGoogle Scholar
  4. Biberfeld, P., Wâhlin, B., Perlmann, P., Biberfeld, G.: A plaque technique for assay and characterization of antibody-dependent cytotoxic effector (K) cells. Scand. J. Immunol. 4, 859–864 (1975)PubMedGoogle Scholar
  5. Blair, P.B., Lane, M-A.: Non-T cell killing of mammary tumor cells by spleen cells: secretion of antibody and recruitment of cells. J. Immunol. 775, 1, 184–189 (1975)Google Scholar
  6. Blair, P.B., Lane, M-A., Mar, P.: Antibody in the sera of tumorbearing mice that mediates spleen cell cytotoxicity toward autologous tumor. J. Immunol. 116, 3, 606–609 (1976)PubMedGoogle Scholar
  7. Brunner, K.T., Mauel, J., Cerottini, J.C., Chapuis, B.: Quantitative assay of the lytic action of immune lymphoid cells on 51Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs. Immunology 14, 181–196 (1968)PubMedGoogle Scholar
  8. Bubenik, J., Tur ano, A.: Inhibitory effects of immune serum on carcinogenesis in mice neonatally infected with murine sarcoma virus (Harvey). Folia Biol. (Praha) 14, 433–439 (1968)Google Scholar
  9. Calder, E.A., Mcleman, D., Irvine, W.J.: Lymphocyte cytotoxicity induced by preincubation with serum from patients with Hashimoto thyroiditis. Short Communication. Clin. Exp. Immunol. 75, 467–470 (1973)Google Scholar
  10. Calder, E.A., Aitken, R.M., Penhale, W.J., Mcleman, D., Irvine, W.J.: Lymphoid cell- mediated antibody-dependent cytotoxicity in untreated and bursectomized chickens. Clin. Exp. Immunol. 16, 137–140 (1974 a)Google Scholar
  11. Calder, E.A., Urbaniak, S.J., Penhale, W.J., Irvine, W.J.: Characterization of human lymphoid cell-mediated antibody-dependent cytotoxicity (LDAC). Clin. Exp. Immunol. 18, 579–593 (1974 b)PubMedGoogle Scholar
  12. Cerottini, J., Brunner, T.: Cell-mediated cytotoxicity, allograft rejection and tumor immunity. Adv. Immunology 18, 67–132 (1974)CrossRefGoogle Scholar
  13. D’Apice, A.J.F., Morris, P.J.: The role of antibody-dependent cell-mediated cytotoxicity in renal allograft rejection. Transplantation 18, 1, 20–26 (1974)PubMedCrossRefGoogle Scholar
  14. Deinhardt, F.: Herpesvirus saimiri. In: The Herpesviruses. Kaplan, A.S. (ed.). New York: Academic Press 1973, pp. 595–625Google Scholar
  15. Delandazuri, M.O., Kedar, E., Fahey, J.L.: Antibody-dependent cellular cytotoxicity to a syngeneic gross virus-induced lymphoma. J. Natl. Cancer Inst. 52, 1, 147–152 (1974 a)Google Scholar
  16. Delandazuri, M.O., Kedar, E., Fahey, J.L.: Synergistic cooperation between isoantiserum and immune lymphoid cells: in vitro studies with a syngeneic rat lymphoma. J. Immunol. 772, 6, 2102–2110 (1974b)Google Scholar
  17. Dennert, G., Lennox, E.S.: Phagocytic cells as effectors in a cell-mediated immunity system. J. Immunol. III, 6, 1844–1854 (1973)Google Scholar
  18. Devries, J.E., Cornain, S., Rümke, P.: Cytotoxicity of non-T versus T-lymphocytes from melanoma patients and healthy donors on short- and long-term cultured melanoma cells. Int. J. Cancer 14, 427–434 (1974)CrossRefGoogle Scholar
  19. Dickmeiss, E.: Comparative study of antibody-dependent and direct lymphocyte-mediated cytotoxicity in vitro after alloimmunization in the human 2. Chemical inhibitors. Scand. J. Immunol. 3, 817–821 (1974)PubMedCrossRefGoogle Scholar
  20. Dukor, P., Bianco, C., Nussenzweig, V.: Tissue localization of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. Proc. Natl. Acad. Sci. U.S.A. 67, 991–997 (1970)PubMedCrossRefGoogle Scholar
  21. Durantez, A., Zighelboim, J.: Studies of lymphocyte-dependent antibodies to leukemia- associated antigens using frozen stored leukemia target cells. Transplantation 22, 2, 190–196 (1976)PubMedCrossRefGoogle Scholar
  22. Fefer, A.: Immunotherapy and chemotherapy of Moloney sarcoma virus induced tumors in mice. Cancer Res. 29, 2177–2183 (1969)PubMedGoogle Scholar
  23. Fefer, A.: Immunotherapy of primary Moloney sarcoma virus-induced tumors. Int. J. Cancer 5, 327–337 (1970)PubMedCrossRefGoogle Scholar
  24. Fefer, A., McCoy, J.L., Glynn, J.P.: Studies on the growth and regression of a transplantable Moloney sarcoma. Cancer Res. 27, 2202–2211 (1967)Google Scholar
  25. Feldmann, J.-L., Becker, M.J., Moutsopoulos, H., Fye, K., Blackman, M., Epstein, W.V., Talal, N.: Antibody-dependent cell-mediated cytotoxicity in selected auto-immune diseases. J. Clin. Invest. 58, 173–179 (1976)PubMedCrossRefGoogle Scholar
  26. Forman, J., Britton, S.: Heterogeneity of the effector cells in the cytotoxic reaction against allogeneic lymphoma cells. J. Exp. Med. 137, 369–386 (1973)PubMedCrossRefGoogle Scholar
  27. Forman, J., Möller, G.: The effector cell in antibody-induced cell mediated immunity. Transplant. Rev. 77, 108–149 (1973)Google Scholar
  28. Forman, J., Britton, S., Möller, G.: Inability of normal and activated thymus-derived cells to act as cytotoxic effector cells against antibody-coated targets. Transplantation 22, 4, 374–379 (1976)PubMedCrossRefGoogle Scholar
  29. Fuson, E.W., Lamon, E.W.: IgM-induced cell-mediated cytotoxicity with antibody and effector cells of human origin. J. Immunol. 118, 1907–1909 (1977)PubMedGoogle Scholar
  30. Garovoy, M.R., Strom, T.B., Kaliner, M., Carpenter, C.B.: Antibody-dependent lymphocyte- mediated cytotoxicity mechanism and modulation by cyclic nucleotides. Cell. Immunol. 20, 197–204 (1975)PubMedCrossRefGoogle Scholar
  31. Garovoy, M.R., Strom, T.B., Gribik, M., Carpenter, C.B.: Antibody-dependent lymphocyte- mediated cytotoxicity (Ab-LMC), definition of the “K cell” in the rat. Transplantation 22, 4, 367–373 (1976)PubMedCrossRefGoogle Scholar
  32. Gelfand, E.W., Resch, K.: Antibody and cell requirements for cytotoxicity by non-immune effector cells. Adv. Exp. Med. Biol. 29, 581–588 (1973)PubMedGoogle Scholar
  33. Gelfand, E.W., Resch, K., Prester, M.: Antibody-mediated target cell lysis by non-immune cells. Characterization of the antibody and effector cell populations. Eur. J. Immunol. 2, 5, 419–424 (1972)PubMedCrossRefGoogle Scholar
  34. Greenberg, A.H., Shen, L.: Class of specific cytotoxic cells demonstrated in vitro by arming with antigen-antibody complexes. Nature (New Biol.) 245, 282–285 (1973)Google Scholar
  35. Greenberg, A.H., Shen, L., Roitt, I.M.: Characterization of the antibody-dependent cytotoxic cell — a non-phagocytic monocyte? Clin. Exp. Immunol. 75, 251–259 (1973)Google Scholar
  36. Greenberg, A.H., Hudson, L., Shen, L., Roitt, I.M.: Antibody-dependent cell-mediated cytotoxicity due to a “null” lymphoid cell. Nature (New Biol.) 242, 111–113 (1973)CrossRefGoogle Scholar
  37. Greenberg, A.H., Shen, L., Walker, L., Arnaiz-Villena, A., Roitt, I.M.: Characteristics of the effector cells mediating cytotoxicity against antibody-coated target cells. II. The mouse nonadherent K cell. Eur. J. Immunol. 5, 474–480 (1975)CrossRefGoogle Scholar
  38. Hakala, T.R., Lange, P.H., Castro, A.E., Elliott, A.Y., Fraley, E.E.: Antibody induction of lymphocyte-mediated cytotoxicity against human transitional-cell carcinomas of the urinary tract. N. Engl. J. Med. 297, 637–641 (1974)CrossRefGoogle Scholar
  39. Hakala, T.R., Lange, P.H., Castro, A.E., Elliott, A.Y., Fraley, E.E.: Lymphocyte antibody interaction in cytotoxicity against human transitional-cell carcinoma. J. Urol. 113, 663–667 (1975)PubMedGoogle Scholar
  40. Hammarström, S., Hellström, U., Perlmann, P., Dillner, M.L.: A new surface marker on T lymphocytes of human peripheral blood. J. Exp. Med. 138, 1270–1275 (1973)PubMedCrossRefGoogle Scholar
  41. Harada, M., Pearson, G., Pettigrew, H., Redmon, L., Orr, T.: Enhancement of normal lymphocyte cytotoxicity by sera with high antibody titiers against H-2 or virus-associated antigens. Cancer Res. 33, 2886–2893 (1973)PubMedGoogle Scholar
  42. Harada, M., Pearson, G., Redmon, L., Winters, E., Kasuga, S.: Antibody production and interaction with lymphoid cells in relation to tumor immunity in the Moloney sarcoma virus system. J. Immunol. 114, 4, 1318–1322 (1975)PubMedGoogle Scholar
  43. Harding, B., Pudifin, DJ., Gotch, F., Maclennan, I.C.M.: Cytotoxic lymphocytes from rats depleted of thymus processed cells. Nature (New Biol.) 232, 80–81 (1971)CrossRefGoogle Scholar
  44. Hellström, J., Hellström, K.E., Sjögren, H.O., Warner, G.A.: Demonstration of cell-mediated immunity to human neoplasms of various histological types. Int. J. Cancer 7, 1–16 (1971)PubMedCrossRefGoogle Scholar
  45. Hellström, U., Hammarström, S., Dillner, M.L., Perlmann, H., Perlmann, P.: Fractionation of human blood lymphocytes on Helix pomatin, a hemagglutin coupled to Sepharose beads. Scand. J. Immunol. 5 (Suppl. 5), 45–54 (1976)Google Scholar
  46. Hershey, P., Culeen, P., Maclennan, I.C.M.: Lymphocyte-dependent cytotoxic antibody activity against human transplantation antigens. Transplantation 16, 9–16 (1973)CrossRefGoogle Scholar
  47. Holm, B., Perlmann, P.: Quantitative studies on phytohaemagglutinin-induced cytotoxicity by human lymphocytes against homologous cells in tissue culture. Immunology 12, 525–536 (1967)PubMedGoogle Scholar
  48. Isturiz, M.A., Debracco, M.M., Pizzi, A.M., Manni, J.A.: Antibody-dependent cell-mediated cytotoxicity in rheumatoid arthritis. Effect of rheumatoid serum fraction on normal lymphocytes. Arthritis Rheum. 19, 4, 725–730 (1976)PubMedCrossRefGoogle Scholar
  49. Jeannet, M., Vassalli, P.: The role of lymphocyte-dependent antibody in kidney transplantation. Transplantation 22, 5, 493–499 (1976)PubMedCrossRefGoogle Scholar
  50. Jewell, D.P., Maclennan, I.C.M.: Circulating immune complexes in inflammatory bowel disease. Clin. Exp. Immunol. 14, 219–226 (1973)PubMedGoogle Scholar
  51. Kay, H.D., Bonnard, G.D., West, W.H., Herberman, R.B.: A functional comparison of human Fc-receptor-bearing lymphocytes active in natural cytotoxicity and antibody- dependent cellular cytotoxicity. J. Immunol. 118, 2058–2066 (1977)PubMedGoogle Scholar
  52. Kiuchi, M., Takasugi, M.: The nonselective cytotoxic cell (N cell). J. Natl. Cancer Inst. 56, 3, 575–582 (1976)PubMedGoogle Scholar
  53. Klein, G., Perlmann, P.: In vitro cytotoxic effect of isoantibody measured as isotope release from labelled target cell DNA. Nature (Lond.) 199, 451–453 (1963)CrossRefGoogle Scholar
  54. Kovithanvongs, T., Dossetor, J.B.: Antibody-induced cytotoxicity by nonsensitized lymphocytes in humans. Transplant. Proc. 5, 4, 1739–1741 (1973)Google Scholar
  55. Kreth, H.W., Ter Meulen, V.: Cell-mediated cytotoxicity against measles virus in SSPE. I. Enhancement by antibody. J. Immunol. 118, 1, 291–295 (1977)PubMedGoogle Scholar
  56. Kumar, S., Waghe, M., Taylor, G.: Tumour-specific antibodies reactive with cell-surface antigens in children with Wilms’tumour. Int. J. Cancer 19, 351–355 (1977)PubMedCrossRefGoogle Scholar
  57. Lamon, E.W., Wigzell, H., Klein, E., Andersson, B., Skurzak, H.M.: The lymphocyte response to primary Moloney sarcoma virus tumors: definition of the active subpopulations at different times after infection in Balb/C mice. J. Exp. Med. 137, 1472–1493 (1973)PubMedCrossRefGoogle Scholar
  58. Lamon, E.W., Andersson, B., Wigzell, H., Fenyo, E.M., Klein, E.: The immune response to primary Moloney sarcoma virus tumors in Balb/C Mice: cellular and humoral activity of long-term regressors. Int. J. Cancer 13, 91–104 (1974)PubMedCrossRefGoogle Scholar
  59. Lamon, E.W., Skurzak, H.M., Andersson, B., Whitten, H.D., Klein, E.: Antibody-dependent lymphocyte cytotoxicity in the murine sarcoma virus system: activity of IgM and IgG with specificity for MLV determined antigen(s). J. Immunol. 114, 4, 1171–1176 (1975a)PubMedGoogle Scholar
  60. Lamon, E.W., Whitten, H.D., Skurzak, H.M., Andersson, B., Lidin, B.: IgM antibody- dependent cell-mediated cytotoxicity in the Moloney sarcoma virus system: the involvement of T and B lymphocytes as effector cells. J. Immunol. 115, 5, 1288–1294 (1975b)PubMedGoogle Scholar
  61. Lamon, E.W., Whitten, H.D., Lidin, B., Fudenberg, H.H.: IgM-induced tumor cell cytotoxicity mediated by normal thymocytes. J. Exp. Med. 142, 542–547 (1975c)PubMedCrossRefGoogle Scholar
  62. Lamon, E.W., Hale, P., Whitten, H.D.: Antibody-dependent cell-mediated cytotoxicity with autochthonous lymphocytes and sera following Moloney sarcoma virus 1 infection. J. Natl. Cancer Inst. 56, 349–355 (1976)PubMedGoogle Scholar
  63. Lamon, E.W., Andersson, B., Whitten, H.D., Hurst, M.M., Ghanta, V.: IgM complex receptors on subpopulations of murine lymphocytes. J. Immunol. 116, 1199–1203 (1976)PubMedGoogle Scholar
  64. iMmon, E.W., Shaw, M.W., Goodson, S., Lidin, B., Walia, A.S., Fuson, E.W.: Antibody- dependent cell-mediated cytotoxicity in the Moloney sarcoma virus system: differential activity of IgG and IgM with different subpopulations of lymphocytes. J. Exp. Med. 145, 302–313 (1977)CrossRefGoogle Scholar
  65. Larsson, J., Ôhlander, C.: In vitro target cell lysis mediated by normal human lymphocytes (K cells) or monocytes. Induction with guinea pig IgGl or IgG2 antibodies. Scand. J. Immunol. 4, 769–776 (1975)PubMedGoogle Scholar
  66. Larsson, Å., Perlmann, P., Natvig, J.B.: Cytotoxicity of human lymphocytes induced by rabbit antibodies to chicken erythrocytes. Inhibition by normal IgG and by human myeloma proteins of different IgG subclasses. Immunology 25, 675–686 (1973)PubMedGoogle Scholar
  67. Larsson, Å., Ôhlander, C., Perlmann, P.: In vitro target cell lysis mediated by normal human lymphocytes. Influence of molecular properties and affinity of inducing antibody. Scand. J. Immunol. 4, 641–652 (1975)PubMedCrossRefGoogle Scholar
  68. Larsson, Å., Pisarri-Salsano, S., Ôhlander, C., Natvig, J.B., Perlmann, P.: Destruction of dextran-coated target cells by normal human lymphocytes and monocytes. Induction by human antidextran serum with IgG antibodies restricted to the IgG2 subclass. Scand. J. Immunol. 4, 241–252 (1975)PubMedCrossRefGoogle Scholar
  69. Lausch, R.N., Sofranko, J., Prather, S.O.: Analysis of sera from SV40-immunized and tumor-bearing hosts for blocking activity and antibody-dependent cellular cytotoxicity. J. Immunol. 755, 3, 682–687 (1975)Google Scholar
  70. Law, L.W., Ting, R.C., Stanton, M.F.: Some biological, immunogenic and morphologic effects in mice after infection with a murine sarcoma virus. I. Biological and immunogenic studies. J. Natl. Cancer Inst 40, 1101–1112 (1968)Google Scholar
  71. Maclennan, I.C.M.: Antibody in the induction and inhibition of lymphocyte cytotoxicity. Transplant. Rev. 13, 67–90 (1972)PubMedGoogle Scholar
  72. Maclennan, I.C.M., Loewi, G.: The effect of specific antibody to target cells on their specific and non-specific interactions with lymphocytes. Nature (Lond.) 219, 1069–1070 (1968)CrossRefGoogle Scholar
  73. MacConnell, 1., Hurd, C.M.: Lymphocyte receptors. II. Receptors for rabbit IgM on human T-lymphocytes. Immunology 30, 835–839 (1976)Google Scholar
  74. Mikulski, S., Billing, R.: Inhibition of lymphocyte-dependent antibody effector cell function by human anti-B lymphocyte alloantiserum. Cell. Immunol. 28, 69–74 (1977)PubMedCrossRefGoogle Scholar
  75. Moller, E.: Contact-induced cytotoxicity by lymphoid cells containing foreign isoantigens. Science 147, 873–879 (1965)CrossRefGoogle Scholar
  76. Moller, G., Svehag, S.-E.: Specificity of lymphocyte-mediated cytotoxicity induced by in vitro antibody-coated target cells. Cell. Immunol. 4, 1–19 (1972)PubMedCrossRefGoogle Scholar
  77. Moretta, L., Ferrarini, M., Durante, M.L., Mingari, M.C.: Expression of a receptor for IgM by human T-cells in vitro. Eur. J. Immunol. 5, 565–569 (1975)PubMedCrossRefGoogle Scholar
  78. Nelson, D.L., Bundy, B.M., Pitchon, H.E., Blaese, RM., Strober, W.: The effector cells in human peripheral blood mediating mitogen-induced cellular cytotoxicity and antibody-dependent cellular cytotoxicity. J. Immunol. III, 5 (Part 1), 1472–1481 (1976)Google Scholar
  79. O’Kafor, G.O., Turner, M.W., Hay, F.C.: Localization of monocyte binding site of human immunoglobulin G. Nature (Lond.) 248, 228–230 (1974)CrossRefGoogle Scholar
  80. O’Toole, C.: A chromium isotope release assay for detecting cytotoxicity to human bladder carcinoma. Int. J. Cancer 19, 324–331 (1977)PubMedCrossRefGoogle Scholar
  81. O’Toole, C., Perlmann, P., Wigzell, H., Unsgaard, B., Zetterlund, C.G.: Lymphocyte cytotoxicity in bladder cancer no requirement for thymus-derived effector cells? Lancet 1973/1, 1085–1088CrossRefGoogle Scholar
  82. O’Toole, C., Stejskal, V., Perlmann, P., Karlsson, M.: Lymphoid cells mediating tumor- specific cytotoxicity to carcinoma of the urinary bladder. Separation of the effector population using a surface marker. J. Exp. Med. 139, 457–466 (1974)PubMedCrossRefGoogle Scholar
  83. Pape, G.R., Troye, M., Perlmann, P.: Characterization of cytolytic effector cells in peripheral blood of healthy individuals and cancer patients. I. Surface markers and K cell activity after separation of B cells and lymphocytes with Fc-receptors by column fractionation. J. Immunol. 118, 1919–1924 (1977a)PubMedGoogle Scholar
  84. Pape, G.R., Troye, M., Perlmann, P.: Characterization of cytolytic effector cells in peripheral blood of healthy individuals and cancer patients. II. Cytotoxicity to allogeneic or autochthonous tumor cells in tissue culture. J. Immunol. 118, 1925–1930 (1977 b)Google Scholar
  85. Pearson, G.R., Davis, S.: Immune response of monkeys to lymphotrophic herpesvirus antigens. Cancer Res. 36, 688–691 (1974)Google Scholar
  86. Pearson, G.R., Orr, T.W.: Antibody-dependent lymphocyte cytotoxicity against cells expressing Epstein-Barr virus antigen. J. Natl. Cancer Inst. 56, 3, 485–488 (1976)PubMedGoogle Scholar
  87. Pearson, G.R., Redmon, L.W., Bass, L.R.: Protective effect of immune sera against transplantable Moloney virus-infected sarcoma and lymphoma. Cancer Res. 33, 171–178 (1973)PubMedGoogle Scholar
  88. Pearson, G.R., Redmon, L.W., Pearson, J.W.: Serochemotherapy against a Moloney virus- induced leukemia. Cancer Res. 33, 1854–1857 (1973)PubMedGoogle Scholar
  89. Pearson, G.R., Prévost, J.M., Orr, T.W.: Detection of virus associated antigens with the antibody-dependent lymphocyte cytotoxicity test. Proc. Int. Workshop on Production and Purification of EBV and Associated Antigens, IARC Monograph (Lyon, France), pp. 263–278 (1975)Google Scholar
  90. Perlmann, P.: Lymphocyte cytotoxicity: technical aspects. Introduction. Transplant. Proc. 4, 295–298 (1972)Google Scholar
  91. Perlmann, P.: Cellular immunity: antibody-dependent cytotoxicity (K-cell activity)In: Clinical Immunology. III. Bach, F.H., Good, R.A. (eds.). New York: Academic Press 1976, pp. 107–132Google Scholar
  92. Perlmann, P., Holm, G.: Cytotoxic effect of lymphoid cells. Adv. Immunol. 77, 117–181 (1969)CrossRefGoogle Scholar
  93. Perlmann, P., Perlmann, H.: Contactual lysis of antibody-coated chicken erythrocytes by purified lymphocytes. Cell. Immunol. 1, 300–315 (1970)PubMedCrossRefGoogle Scholar
  94. Perlmann, P., Perlmann, H., Biberfeld, P.: Specific cytotoxic lymphocytes produced by preincubation with antibody-complexed target cells. J. Immunol. 108, 558–561 (1972)PubMedGoogle Scholar
  95. Perlmann, P., Perlmann, H., Wigzell, H.: Lymphocyte mediated cytotoxicity in vitro induction and inhibition by humoral antobody and nature of effector cells. Transplant. Rev. 13, 91–114 (1972)PubMedGoogle Scholar
  96. Perlmann, P., Holm, G., Stejskal, V.: Cell-mediated cytotoxicity in vitro: target cell recognition and effector-cell requirements. Transplant. Proc. 5, 4, 1625–1629 (1973)PubMedGoogle Scholar
  97. Perlmann, P., Wigzell, H., Goldstein, P., Lamon, E.W., Larsson, Å., O’Toole, C., Perlmann, H., Svedmyr, E.A.J.: Cellmediated cytolysis in vitro. Analysis of active lymphocyte subpopulations in different experimental systems. Adv. Bioscience 72, 71–85 (1974)Google Scholar
  98. Perlmann, P., Perlmann, H., Larsson, Å., Wáhlin, B.: Antibody dependent cytolytic effector lymphocytes (K cells) in human blood. J. Reticuloendothel. Soc. 77, 4, 241–250 (1975)Google Scholar
  99. Perlmann, H., Perlmann, P., Pape, G.R., Hallden, G.: Purification, fractionation and assay of antibody-dependent lymphocytic effector cells (K cells) in human blood. Scand. J. Immunol. 5 (Suppl. 5), 57–68 (1976)PubMedCrossRefGoogle Scholar
  100. Pollack, S., Nelson, K.: Effects of carrageenan and high serum dilutions on synergistic cytotoxicity to tumor cells. J. Immunol. 110, 1440–1443 (1973)PubMedGoogle Scholar
  101. Pollack, S., Nelson, K.: Evidence for two factors in sera of tumor-immunized mice which induce specific lymphoid cell-dependent cytotoxicity: IgG2 and a rapidly appearing factor not associated with IgG or IgM. Int. J. Cancer 16, 339–346 (1975)PubMedCrossRefGoogle Scholar
  102. Pollack, S., Heppner, G., Brawn, R.J., Nelson, K.: Specific killing of tumor cells in vitro in the presence of normal lymphoid cells and sera from hosts immune to the tumor antigens. Int. J. Cancer. 9, 316–323 (1972)PubMedCrossRefGoogle Scholar
  103. Prather, S.O., Lausch, R.N.: Kinetics of serum factors mediating blocking, unblocking and antibody-dependent cellular cytotoxicity in hamsters given isografts of Para-7 tumor cells. Int. J. Cancer 77, 380–388 (1976)CrossRefGoogle Scholar
  104. Prevost, J.-M., Orr, T.W., Pearson, G.R.: Augmentation of lymphocyte cytotoxicity by antibody to Herpesvirus saimiri associated antigens. Proc. Natl. Acad. Sci. U.S.A. 72, 5, 1671–1675 (1975)PubMedCrossRefGoogle Scholar
  105. Prevost, J.-M., Pearson, G., Wallen, W.C., Rabin, H., Qualtiere, L.F.: Antibody-responses to membrane antigens in monkeys infected with Herpesvirus saimiri. Int. J. Cancer 18, 679–686 (1976)PubMedCrossRefGoogle Scholar
  106. Pross,H.F., Tracey, D.E., Wigzell, H., Schirrmacher, V.: Antibody-dependent cell-mediated cytotoxicity in the mouse. I. Surface characteristics of an effector cell. Scand. J. Immunol. 3, 6, 769–780 (1974)Google Scholar
  107. Rager-Zisman, B., Allison, A.C.: Mechanism of immunologic resistance to Herpes simplex virus 1 (HSV-1) infection. J. Immunol. 116, 1, 35–40 (1976)PubMedGoogle Scholar
  108. Rager-Zisman, B., Bloom, B.: Immunological destruction of Herpes simplex 1 infected cells. Nature (Lond.) 251, 542–543 (1974)CrossRefGoogle Scholar
  109. Resch, K., Gelfand, E.W., Prester, M.: Antibody-mediated target cell lysis by non-immune cells: the use of anti-immunoglobulin to distinguish effector cell populations. J. Immunol. 112, 791–803 (1974)Google Scholar
  110. Roitt, I.M., Shen, L., Greenberg, A.H.: Antibody-dependent cell-mediated cytotoxicity. In: The Role of Immunological Factors in Infectious, Allergic, and Autoimmune Processes. Beers, R.F., Jr., Bassett, E.G. (eds.). New York: Raven Press 1976, pp. 281–288Google Scholar
  111. Rosenberg, E.B.: Lymphocyte-dependent antibodies. Lancet 1973/1, 154PubMedCrossRefGoogle Scholar
  112. Rouse, B.T., Wardley, R.C., Babiuk, L.A.: The role of antibody-dependent cytotoxicity in recovery from herpesvirus infections. Cell. Immunol. 22, 182–186 (1976)PubMedCrossRefGoogle Scholar
  113. Saal, J.G., Rieber, E.P., Hadam, M., Riethmuller, G.: Lymphocytes with T-cell markers2) cooperate with IgG antibodies in the lysis of human tumour cells. Nature (Lond.) 265, 5590, 158–169 (1977)CrossRefGoogle Scholar
  114. Saksela, E., Imir, T., Makela, 0.: Specifically cytotoxic human and mouse lymphoid cells induced with antibody or antigen-antibody complexes. J. Immunol. 115, 6, 1488–1492 (1975)PubMedGoogle Scholar
  115. Sanderson, C.J., Clark, I.A., Taylor, G.A.: Different effector cell types in antibody-dependent cell-mediated cytotoxicity. Nature (Lond.) 253, 376–377 (1975)CrossRefGoogle Scholar
  116. Scornick, J.C.: Antibody-dependent cell-mediated cytotoxicity. II. Early interactions between effector and target cells. J. Immunol. 113, 5, 1519–1526 (1974)Google Scholar
  117. Scornick, J.C., Cosenza, H., Lee, W., Kohler, H., Rowley, D.A.: Antibody-dependent cell- mediated cytotoxicity. I. Differentiation from antibody-independent cytotoxicity by “normal” IgG. J. Immunol. 113, 5, 1510–1518 (1974)Google Scholar
  118. Shimizu, F., Hanaumi, K, Shimizu, Y., Kumagai, K.: Antibody-dependent cellular protection against Herpes simplex virus dissemination as revelaed by viral plaque and infectivity assays. Infect. Immunol. 16, 2, 531–536 (1977)Google Scholar
  119. Shore, S.L., Nahmias, A.J., Starr, S.E., Wood, P.A., McFarlin, D.E.: Detection of cell- dependent cytotoxic antibody to cell infected with Herpes simplex virus. Nature (Lond.) 251, 350–352 (1974)CrossRefGoogle Scholar
  120. Shore, S.L., Cromeans, T.L., Romano, T.J.: Immune destruction of virus-infected cells early in the infectious cycle. Nature (Lond.) 262, 5570, 695–696 (1976)CrossRefGoogle Scholar
  121. Shore, S.L., Black, C.M., Melewicz, F.M., Wood, P.A., Nahmias, A.J.: Antibody-dependent cell-mediated cytotoxicity to target cells infected with type 1 and type 2 Herpes simplex virus. J. Immunol. 116, 1, 194–201 (1976)PubMedGoogle Scholar
  122. Simchowitz, L., Schur, P.H.: Antibody-dependent lymphocyte-mediated cytotoxicity. The nature of the effector cell and characterization of its Fc receptor. Scand. J. Immunol. 5, 759–769 (1976)PubMedCrossRefGoogle Scholar
  123. Skurzak, H.M., Klein, E., Yoshida, T.O., Lamon, E.W.: Synergistic or antagonistic effect of different antibody concentrations on in vitro lymphocyte cytotoxicity in the Moloney sarcoma virus system. J. Exp. Med. 135, 4, 997–1002 (1972)PubMedCrossRefGoogle Scholar
  124. Spiegelberg, H.L., Perlmann, H., Perlmann, P.: Interaction of K lymphocytes with myeloma proteins of different IgG subclasses. J. Immunol. 116, 5, 1464–1471 (1976)Google Scholar
  125. Stiller, C.R., Abrahams, S., Fung, M., Sinclair, N.R. StC., Ulan, R.A., Wallace, A.C.: Lymphocyte-dependent antibody and renal graft injection. Lancet 1975/1 953–954PubMedCrossRefGoogle Scholar
  126. Strom, T.B., Garovoy, M.R., Bear, R.A., Gribik, M., Carpenter, C.B.: A comparison of the effects of metabolic inhibitors upon direct and antibody-dependent lymphocyte mediated cytotoxicity. Cell. Immunol. 20, 247–256 (1975)PubMedCrossRefGoogle Scholar
  127. Takasugi, M., Kinoshita, K: Specific antibody activation of lymphocytes for cell-mediated cytotoxicity against cultured target cells. J. Natl. Cancer Inst. 53, 6, 1539–1544 (1974)PubMedGoogle Scholar
  128. Takasugi, M., Klein, E.: A microassay for cell-mediated immunity. Transplantation 9, 219–227 (1970)PubMedCrossRefGoogle Scholar
  129. Takasugi, M., Mickey, M.R.: Interaction analysis of selective and nonselective cell-mediated cytotoxicity. J. Natl. Cancer Inst. 57, 2, 255–261 (1976)PubMedGoogle Scholar
  130. Takasugi, M., Mickey, M.R., Terasaki, P.I.: Reactivity of lymphocytes from normal persons on cultured tumor cells. Cancer Res. 33, 2898–2902 (1973)PubMedGoogle Scholar
  131. Takasugi, M., Mickey, M.R., Terasaki, P.I.: Studies on specificities of cell-mediated immunity to human tumors. J. Natl. Cancer Inst. 53, 1527–1538 (1974)PubMedGoogle Scholar
  132. Thomas, J.M., Thomas, F.T., Kaplan, A.M., Lee, H.M.: Antibody-dependent cellular cytotoxicity and chronic renal allograft rejection. Transplantation 22, 2, 94–100 (1976)PubMedCrossRefGoogle Scholar
  133. Thomas, J., Thomas, F., Lee, H.M.: Studies of lymphocyte-dependent antibody in human chronic renal allograft rejection. Transplant. Proc. 9, 1, 65–68 (1977)PubMedGoogle Scholar
  134. Ting, A., Terasaki, P.I.: Brief communication. Influence of lymphocyte-dependent antibodies on human kidney transplants. Transplantation 18, 4, 371–373 (1974)PubMedCrossRefGoogle Scholar
  135. Ting, A., Terasaki, P.I.: Lymphocyte-dependent antibody crossmatching for transplant patients. Lancet 1975/1, 304–306PubMedCrossRefGoogle Scholar
  136. Trinchieri, G., Demarchi, M., Mayr, W., Savi, M., Ceppellini, R.: Lymphocyte antibody lymphocy to lytic interaction (LALI) with special emphasis on HL-A. Transplant. Proc. 5, 1631–1646 (1973)PubMedGoogle Scholar
  137. Trinchieri, G., Bauman, P., Demarchi, M., Tôkes, Z.: Antibody-dependent cell-mediated cytotoxicity in humans. I. Characterization of the effector cell. J. Immunol. 775, 1, 249–255 (1975)Google Scholar
  138. InVainio, T., Koskimies, O., Perlmann, P., Perlmann, H., Klein, E.: In vitro cytotoxic effect of lymphoid cells from mice immunized with allogeneic tissue. Nature (Lond.) 204, 453–455 (1964)CrossRefGoogle Scholar
  139. Van Boxel, J.A., Stobo, J.D., Paul, W.E., Green, I.: Antibody-dependent lymphoid cell- mediated cytotoxicity: no requirement for thymus-derived lymphocytes. Science 775, 194–196 (1972)CrossRefGoogle Scholar
  140. Van Boxel, J.A., Paul, W.E., Frank, M.M., Green, J.: Antibody-dependent lymphoid cell- mediated cytotoxicity: role of lymphocytes bearing a receptor for complement. J. Immunol. 110, 1027–1036 (1973)Google Scholar
  141. Van Boxel, J.A., Paul, W.E., Green, I., Frank, M.M.: Antibody-dependent lymphoid cell- mediated cytotoxicity: role of complement. J. Immunol. 772, 1, 398–403 (1974)Google Scholar
  142. Wâhlin, B., Perlmann, H., Perlmann, P.: Analysis by a plaque assay of IgG- or IgM- dependent cytolytic lymphocytes in human blood. J. Exp. Med. 144, 1375–1380 (1976)PubMedCrossRefGoogle Scholar
  143. Wallen, W.C., Pearson, G.R., Prévost, J.-M., Neubauer, R.H., Rabin, H.: Correspondence between T cell suppression and ADLC titers during Herpesvirus saimiri (HVS) infection in owl monkeys. Bibl. Haematol. 43, 339–342 (1975)PubMedGoogle Scholar
  144. Wasserman, J., von Stedingk, L.-V., Perlmann, P., Jonsson, J.: Antibody-induced in vitro lymphocyte cytotoxicity in Hashimoto thyroiditis. Int. Arch. Allergy Appl. Immunol. 47, 473–482 (1974)PubMedCrossRefGoogle Scholar
  145. West, W.H., Cannon, G.B., Kay, H.D., Bonnard, G.D., Herberman, R.B.: Natural cyto toxictivity of human lymphocytes against a myeloid cell line: characterization of effector cells. J. Immunol. 118, 1, 355–361 (1977)PubMedGoogle Scholar
  146. Wigzell, H.: Quantitative tirations of mouse H-2 antibodies using Cr51 -labelled target cells. Transplantation 3, 423–431 (1965)PubMedCrossRefGoogle Scholar
  147. Wisloff, F., Fro land, S.S.: Antibody-dependent lymphocyte mediated cytotoxicity in man: no requirement for lymphocytes with membrane-bound immunoglobulin. Scand. J. Immunol. 2, 151–157 (1973)PubMedCrossRefGoogle Scholar
  148. Wisloff, F., Fro land, S.S., Michaelsen, T.E.: Antibody-dependent cytotoxicity mediated by human Fc-receptor-bearing cells lacking markers for B- and T-lymphocytes. Int. Arch. Allergy Appl. Immunol. 47, 139–154 (1974)PubMedCrossRefGoogle Scholar
  149. Wunderlich, J., Rosenburg, E., Connolly, J., Parks, J.: Characteristics of a cytotoxic human lymphocyte-dependent antibody. J. Natl. Cancer Inst. 54, 3, 537–547 (1975)PubMedGoogle Scholar
  150. Yoshida, T.O., Andersson, B.: Evidence for a receptor recognizing antigen complexed immunoglobulin on the surface of activated mouse thymus lymphocytes. Scand. J. Immunol. 7, 4, 401–408 (1972)CrossRefGoogle Scholar
  151. Yust, J., Wunderlich, J.R., Mann, D.L., Terry, W.D.: Identification of lymphocyte-dependent antibody in sera from multiply transfused patients. Transplantation 18, 2, 99–107 (1974)PubMedCrossRefGoogle Scholar
  152. Zeiglemaker, W.P., Roos, M.T.L., Schellekens, P.T.A., Eijsvoogel, V.P.: Antibody-dependent human lymphocyto toxicity: a microassay system. Eur. J. Immunol. 5, 579–584 (1975)CrossRefGoogle Scholar
  153. Ziegler, H., Henney, C.: Antibody-dependent cytolytically active human leukocytes: an analysis of inactivation following in vitro interaction with antibody-coated target cells. J. Immunol. 775, 6, 1500–1504 (1975)Google Scholar
  154. Zighelboim, J., Bonavida, B., Fahey, J.L.: Evidence for several cell populations active in antibody-dependent cellular cytotoxicity. J. Immunol. III, 6, 1737–1942 (1973)Google Scholar
  155. Zighelboim, J., Gale, R.P., Chiu, A., Bonavida, B., Ossorio, R.C., Fahey, J.L.: Antibody- dependent cellular cytotoxicity: cytotoxicity mediated by non-T lymphocytes. Clin. Immunol. Immunopathol. 3, 193–200 (1974)PubMedCrossRefGoogle Scholar
  156. Zighelboim, J., Gale, R.P., Ossorio, R.C., Fahey, J.L.: A sensitive in vitro microassay for detecting antibody-dependent cellular cytotoxicity. Possible role in monitoring lymphocyte function. Transplantation 18, 1, 27–30 (1974)Google Scholar
  157. Zinkernagel, R.M., Oldstone, M.B.A.: Cells that express viral antigens but lack H-2 determinants are not lysed by immune thymus-derived lymphocytes, but are lysed by other antiviral immune attack mechanisms. Proc. Natl. Acad. Sci. U.S.A. 73, 10, 3666–3670 (1976)PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1978

Authors and Affiliations

  • Gary R. Pearson
    • 1
  1. 1.Mayo Clinic/Foundation and Mayo Medical SchoolRochesterUSA

Personalised recommendations