Smooth Muscle Cell Proliferation and Migration After Removal of Arterial Endothelium in Rabbits
Our experiments relate to Dr. WISSLER’s scheme, in which the desquamation of arterial endothelium is the primary event. Physical and/or chemical injuries such as high shear stress, homocystinemia, or hypercholesterolemia may induce endothelial sloughing. In our experimental model (1, 2), a balloon catheter is introduced into one femoral artery, pushed up into the abdominal aorta, and withdrawn within approximately 6 s. Although this procedure results in an iliac artery denuded of its endothelium, it usually does not cause visible damage to the smooth muscle cells of the media. However, as in any proper experiment a few animals should always be sacrificed to quantify the degree of endothelial denudation and smooth muscle cell damage, i.e., to have a base-line established for the interpretation of the subsequent sequence of events. The iliac artery not touched by the balloon serves as built-in control.
KeywordsCholesterol Migration Catheter Aspirin Luminal
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