Gene Cloning with Small Plasmids

  • John Collins
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 78)


The following account of the use of plasmids in gene cloning is an attempt to present this technique against a background of its subsidiary techniques—plasmid genetics, DNA biochemistry, bacterial transformation etc. —while at the same time considering the ways in which the field is likely to develop in view of the particular aims of different categories of cloning experiments. Rapid progress in this area of research will require regular review articles to keep abreast of new developments. New material was incorporated in this review up to the end of January 1977.


Gene Cloning Hybrid Clone Hybrid Plasmid Bacterial Plasmid Small Plasmid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Ampicillin resistance




complementary DNA


Carbenicillin resistance


Chloramphenicol resistance


double stranded


single stranded


deoxyribonucleotide triphosphate










insertion sequence


Kanamycin resistance


million daltons


ribonucleotide triphosphate










Neomycin resistance


tetracycline resistance


deoxyribonucleotidyl terminal transferase


transfer RNA


temperature sensitive


transposon (TnA=ampicillin transposon) or transposition element


restriction endonuclease EcoRI






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© Springer-Verlag Berlin Heidelberg 1977

Authors and Affiliations

  • John Collins
    • 1
  1. 1.Gesellschaft für Biotechnologische Forschung mbHBraunschweig-StöckheimFederal Republic of Germany

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