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Gene Cloning with Small Plasmids

  • John Collins
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 78)

Abstract

The following account of the use of plasmids in gene cloning is an attempt to present this technique against a background of its subsidiary techniques—plasmid genetics, DNA biochemistry, bacterial transformation etc. —while at the same time considering the ways in which the field is likely to develop in view of the particular aims of different categories of cloning experiments. Rapid progress in this area of research will require regular review articles to keep abreast of new developments. New material was incorporated in this review up to the end of January 1977.

Keywords

Gene Cloning Hybrid Clone Hybrid Plasmid Bacterial Plasmid Small Plasmid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

Ap

Ampicillin resistance

CAM

Chloramphenicol

cDNA

complementary DNA

Cb

Carbenicillin resistance

Cm

Chloramphenicol resistance

ds

double stranded

ss

single stranded

dNTP

deoxyribonucleotide triphosphate

dA

deoxyadenosine

dT

deoxythymidine

dC

deoxycytidine

dG

deoxyguanosine

IS

insertion sequence

Km

Kanamycin resistance

Md

million daltons

NTP

ribonucleotide triphosphate

A

adenosine

U

uridine

C

cytidine

G

guanosine

Nm

Neomycin resistance

Tc

tetracycline resistance

TdT

deoxyribonucleotidyl terminal transferase

tRNA

transfer RNA

ts

temperature sensitive

Tn

transposon (TnA=ampicillin transposon) or transposition element

EcoRI

restriction endonuclease EcoRI

X

purine

Y

pyrimidine

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References

  1. Adhya, S., Gottesman, M., De Crombrugghe, B.: Release of polarity in Escherichia coli by gene N of phage X: termination and antitermination of transcription. Proc. Natl. Acad. Sci. U.S.A. 71, 2534–2538 (1974)PubMedGoogle Scholar
  2. Akagawa, H., Okanishi, M., Umezawa, H.: A plasmid involved in chloramphenicol production in Streptomyces venezuelae: evidence from genetic mapping. J. Gen. Microbiol. 90, 336–346 (1975)PubMedGoogle Scholar
  3. Anagnostopoulus, C., Spizizen, J.: Requirements for transformation in Bacillus subtilis. J. Bacteriol. 81, 741–746 (1961)Google Scholar
  4. Anderson, E.S.: The ecology of transferable drug resistance in Enterobacteria. Annu. Rev. Microbiol. 22, 131–180 (1968)PubMedGoogle Scholar
  5. Anderson, J.N., Schimke, R.T.: Partial purification of the ovalbumin gene. Cell 7, 331–338 (1976)PubMedGoogle Scholar
  6. Arber, W., Linn, S.: DNA modification and restriction. Annu. Rev. Biochem. 38, 467–500 (1969)PubMedGoogle Scholar
  7. Arber, W.: DNA modification and restriction. Prog. Nucleic Acid Res. Mol. Biol. 14, 1–50 (1974)PubMedGoogle Scholar
  8. Ausubel, F.M., Cannon, F.C., Riedel, R.: Cloning of his and nif genes from Klebsiella pneumoniae. Personal communication (1976)Google Scholar
  9. Avery, O.T., Meod, C.M., Marty, M.: Studies on the chemical nature of the substance inducing transformation of Pneumococcal types. J. Exp. Med. 79, 137–158 (1944)PubMedGoogle Scholar
  10. Berg, D.E., Davies, J., Allet, B., Rochaix, J.-D.: Transposition of R factor genes to bacteriophage X. Proc. Natl. Acad. Sci. U.S.A. 72, 3628–3632 (1975)PubMedGoogle Scholar
  11. Berg, P.E., Gay da, R., Avni, H., Zehnbauer, B., Markovitz, A.: Cloning of Escherichia coli DNA that controls cell division and capsular polysaccharide synthesis. Proc. Natl. Acad. Sci. U.S.A. 73, 697–701 (1976)PubMedGoogle Scholar
  12. Bigger, C.H., Murray, K, Murray, N.E.: Recognition sequence of a restriction enzyme. Nature [New Biol.] 244, 7–10 (1973)Google Scholar
  13. Blair, D. G., Sherratt, D. J., Clewell, D. B., Helinski, D. R.: Isolation of supercoiled colicinogenic factor EI DNA sensitive to ribonuclease and alkali. Proc. Natl. Acad. Sci. U.S.A. 69, 2518–2522 (1972)PubMedGoogle Scholar
  14. Blin, N., Gabain, A.V., Bujard, H.: Isolation of large molecular weight DNA from agarose gels for further digestion by restriction enzymes. FEBS Lett. 53, 84–86 (1975)PubMedGoogle Scholar
  15. Bolivar, F., Rodriguez, R. L., Greene, P. J., Betlach, M. C., Heyneker, H. L., Boyer, H. W., Crosa, J. H., Falkows, S.: Construction and characterisation of a new E. coli plasmid, pBR322, serving as a cloning vehicle in an EK2 system. Gene (1977) in press.Google Scholar
  16. Bollum, F. J.: Terminal deoxynucleotidyl transferase. In: The Enzymes. Boyer, P.D. (ed.). New York: Academic Press 1974, Vol. X, pp. 145–171Google Scholar
  17. Borst, P., Aaij, C.: Identification of the heavy strand of rat liver mitochondrial DNA as the messenger strand. Biophys. Res. Commun. 34, 358–364 (1969)Google Scholar
  18. Boyer, H.W., Chow, L.T., Dugaiczyk, A., Hedgpeth, J., Goodman, H.M.: DNA substrate site for the Ecll restriction endonuclease and modification methylase. Nature [New Biol.] 224, 40–43 (1973)Google Scholar
  19. Brammar, W.J., Murray, N.E., Winton, S.: Restriction of k trp bacteriophages by Escherichia coli K. J. Molec. Biol. 90, 633–647 (1974)PubMedGoogle Scholar
  20. Brown, D.D., Stern, R.: Methods of gene isolation. Annu. Rev. Biochem. 43, 667–693 (1974)PubMedGoogle Scholar
  21. Brown, W.M., Watson, R.M., Vinograd, J., Tait, K.M., Boyer, H.W., Goodman, H.W.: The structure and fidelity of replication of Mouse mitochondrial DNA-C101 Ecl recombinant plasmids grown in E. coli K12. Cell. 7, 517–530 (1976)PubMedGoogle Scholar
  22. Bursztyn, H., Sgaramella, V., Ciferri, O., Lederberg, J.: Transfectability of rough strains of Salmonella typhimunium. J. Bacteriol. 124, 1630–1634 (1975)PubMedGoogle Scholar
  23. Cabello, F., Timmis, K., Cohen, S.N.: Replication control in a somposite plasmid constructed by in vitro linkage of two distinct replicons. Nature (Lond.) 259, 285–290 (1976)Google Scholar
  24. Cameron, J. R., Panasenko, S. M., Lehman, L. R., Davis, R.W.: In vitro construction of bacteriophage k carrying segments of the Escherichia coli chromosome: selection of hybrids containing the gene for DNA ligase. Proc. Natl. Acad. Sci. U.S.A. 72, 3416–3420 (1975)PubMedGoogle Scholar
  25. Cannon, F.C., Dixon, R.A., Postgate, J.R., Primrose, S.B.: Plasmids formed in nitrogen fixing Escherichia coli—Klebsiella pneumoniae hybrids. J. Gen. Microbiol. 80, 241–251 (1974)PubMedGoogle Scholar
  26. Carbon, J., Clarke, L., Ilgen, C., Ratzkin, B.: The construction and use of hybrid plasmid gene banks in Escherichia coli. In: Tenth Miles Symposium Cambridge, Mass., June 8–10, 1976 (in Press)Google Scholar
  27. Cavalli-Sforza, L., Lederberg, J.: Isolation of pre-adaptive mutants in bacteria by sib selection. Genetics 41, 367–381 (1956)PubMedGoogle Scholar
  28. Chakrabarty, A.M., Mylroie, J.R., Friello, D.A., Vacca, J.G.: Transformation of Pseudomonas putida and Escherichia coli with plasmid-linked drug-resistance factor DNA. Proc. Natl. Acad. Sci. U.S.A. 72, 3647–3651 (1975)PubMedGoogle Scholar
  29. Chakrabarty, A.M.: Molecular cloning in Pseudomonas. In: Microbiology American Society for Microbiology (Washington) 1976 (in press)Google Scholar
  30. Chandler, M. G., Pritchard, R. H.: The effect gene concentration and relative gene dosage on gene output in Escherichia coli. Molec. Gen. Genet. 138, 127–141 (1975)PubMedGoogle Scholar
  31. Chang, A.C.Y., Cohen, S.N.: Genome construction between bacterial species in vitro: replication and expression of Staphylococcus plasmid genes in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 71, 1030–1034 (1974)PubMedGoogle Scholar
  32. Chang, A.C.Y., Lansman, R.A., Clayton, D.A., Cohen, S.N.: Studies of Mouse mitochondrial DNA in Escherichia coli: Structure and function of the eukaryotic-prokaryotic chimeric plasmids. Cell 6, 231–244 (1975)PubMedGoogle Scholar
  33. Clarke, L., Carbon, J.: Biochemical construction and selection of hybrid plasmids containing specific segments of the Escherichia coli genome. Proc. Natl. Acad. Sci. U.S.A. 72, 4361–4365 (1975)PubMedGoogle Scholar
  34. Clarke, L., Carbon, J.: A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9, 91–99 (1976)PubMedGoogle Scholar
  35. Clewell, D.B., Helinski, D.R.: Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochem. 9, 4428–4440 (1970)Google Scholar
  36. Clewell, D.B.: Nature of Col plasmid replication in Escherichia coli in the presence of chloramphenicol. J. Bacteriol. 110, 667–673 (1972)PubMedGoogle Scholar
  37. Clewell, D.B., Evenchik, B., Cranston, J.W.: Direct inhibition of Col El plasmid replication in Escherichia coli by rifampicin. Nature [New Biol.] 237, 29–31 (1972)Google Scholar
  38. Clowes, R.C.: Molecular structure of bacterial plasmids. Bacteriol. Rev. 36, 361–405 (1972)PubMedGoogle Scholar
  39. Cohen, S. N., Chang, A. C. Y., Hsu, L.: Nonchromosomal antibiotic resistance in bacteria: Genetic transformation of Escherichia coli by R-factor DNA. Proc. Natl. Acad. Sci. U.S.A. 69, 2110–2114 (1972)PubMedGoogle Scholar
  40. Cohen, S.N., Chang, A.C.Y.: Recircularisation and autonomous replication of a sheared R-factor DNA segment in Escherichia coli transformants. Proc. Natl. Acad. Sci. U.S.A. 70, 1293–1297 (1973)PubMedGoogle Scholar
  41. Cohen, S.N., Chang, A.C.Y., Boy er, H.W., Helling, R.B.: Construction of biologically functional bacterial plasmids in vitro. Proc. Natl. Acad. Sci. U.S.A. 70, 3240–3244 (1973)PubMedGoogle Scholar
  42. Cohn, R.H., Lowry, J.C., Kedes, L.H.: Histone genes of the Sea Urchin (S. purpuratus) cloned in E. coli: order, polarity and strandedness of the five histone-coding and spacer regions. Cell 9, 147–161 (1976)PubMedGoogle Scholar
  43. Collins, J., Lincoln, D. N.: A computer program to give potential endonuclease sites within genes for proteins of known amino-acid sequence, (unpublished; available on request from author)Google Scholar
  44. Collins, J., Pritchard, R.H.: Relationship between chromosome replication and F ’lac epi-some replication in Escherichia coli. J. Molec. Biol. 78, 143–155 (1973)PubMedGoogle Scholar
  45. Collins, J., Johnsen, M., Jorgensen, P., Valentin-Hansen, P., Karlström, H. O., Gautier, F., Lindenmaier, W., Mayer, H., Sjörherg, B.M.: Expression of plasmid genes: Col and derivatives. American Society for Microbiology; Symposium on Extrachromosomal genetic elements. Jackson Hole (Ed. Novik, R, Davies, J.) (in press 1977)Google Scholar
  46. Collins, J., Jorgensen, P., Karlström, H. O., Lindenmaier, W., Johnsen, M., Sjöberg, B.-M.: Cloning of Escherichia coli genes important in the regulation of ribosome synthesis. In: Gene Insertions. New York: Cold Spring Harbor Publications 1976 (in press)Google Scholar
  47. Collins, J., Fiil, N.P., Jorgensen, P., Friesen, J.D.: Cloning of genes involved in regulation of ribosome synthesis in Escherichia coli. In: Control of Ribosome Synthesis. IX. Alfred Benzon Symposium, 1975, pp. 356–367. Copenhagen: Munksgaard Press 1976Google Scholar
  48. Collins, J., Yanofsky, S., Helinski, D. R.: Involvement of the DNA-protein relaxation complex in the replication of Col. Molec. Gen. Genet, (in press 1977)Google Scholar
  49. Cooper, S., Helmstetter, C. E.: Chromosome replication and the division cycle of Escherichia coli B/r. J. Molec. Biol. 31, 519–540 (1968)PubMedGoogle Scholar
  50. Cosloy, S.D., Oishi, M.: Genetic transformation in Escherichia coli Kl2. Proc. Natl. Acad. Sci. USA 70, 84–87 (1973)PubMedGoogle Scholar
  51. Covey, C., Richardson, D., Carbon, J.: A method for the deletion of restriction sites in bacterial plasmid deoxyribonucleic acid. Molec. Gen. Genet. 145, 155–158 (1976)PubMedGoogle Scholar
  52. Curtiss, R. III: Bacterial conjugation. Annu. Rev. Microbiol. 23, 69–136 (1969)PubMedGoogle Scholar
  53. Curtiss, R. III: Genetic manipulation of microorganisms: Potential benefits and biohasards. Annu. Rev. Microbiol. 30, 507–533 (1976)PubMedGoogle Scholar
  54. Danna, K.J., Sack, G.H., Nathans, D.: Studies of simian virus 40 DNA, VII. A cleavage map of the SV40 genome. J. Molec. Biol. 78, 363–376 (1973)PubMedGoogle Scholar
  55. Dawid, I. B.: Mitochondrial RNA in Xenopus laevis. I. The expression of the mitochondrial genome. J. Molec. Biol. 63, 201–216 (1972a)Google Scholar
  56. Dawid, I. B.: The evolution of mitochondrial DNA sequences in Xenopus. Dev. Biol. 29, 139 (1972b)PubMedGoogle Scholar
  57. De Witt, W., Helinski, D.R.: Characterisation of colicinogenic factor El from a non-induced and a mitomycin C-induced Proteus strain. J. Molec. Biol. 13, 692–703 (1965)Google Scholar
  58. Dixon, R., Cannon, F., Kondor osi, A.: Construction of a P plasmid carrying nitrogen-fixation genes from Klebsiella pneumoniae. Nature (Lond.) 260, 268–271 (1976)Google Scholar
  59. Dugaiczyk, A., Boyer, H.W., Goodman, H.M.: Ligation of Ecl endonuclease-generated DNA fragments into linear and circular structures. J. Molec. Biol. 96, 171–184 (1975)PubMedGoogle Scholar
  60. Dugaiczyk, A., Turner, E. G., Nasser, D., Boyer, H. W., Marthy, B. J., Goodman, H. M.: Cloning of Drosophila melanogaster Ecl DNA fragments. In: ICN-UCLA Symposium on Molecular and Cellular Biology. Vol. V. Nierlich, D. P., Rutter, W. J., Fox, C. F. (eds.). New York: Academic Press 1976Google Scholar
  61. Efstratiadis, A., Kafatos, F.C., Maxam, A.M., Maniatis, T.: Enzymatic in vitro synthesis of Globin genes. Cell 7, 279–288 (1976)PubMedGoogle Scholar
  62. Engberg, B., Nordstrom, K.: Replication of the R-factor R1 in Escherichia coli K12 at different growth rates. J. Bacteriol. 725, 179–186 (1975)Google Scholar
  63. Engberg, J., Andersson, P., Vagn Leick, Collins, J.: Free ribosomal DNA molecules from Tetrahymena pyriformis GL are giant palindromes. J. molec. Biol. 104, 455–470 (1976)PubMedGoogle Scholar
  64. Falkow, S., Haapala, D.K., Silver, R.P.: Relationships between extrachromosomal elements. CIBA Symposium: Bacterial Episomes and Plasmids, pp. 136–158. Wolstenholme, J., O’Connor, A. (eds). London: Churchill 1969Google Scholar
  65. Fareed, G. C., Wilt, E. M., Richardson, C. C.: Enzymatic breakage and joining of deoxyribonucleic acid. iii. Hybrids of ribo and deoxyribonucleotide homopolymers as substrate for polynucleotide ligase of bacteriophage T4. J. Biol. Chem. 246, 925–932 (1971)PubMedGoogle Scholar
  66. Feix, G., Sano, H., Hobom, G.: Integration of a poly (. poly ()) segment into A DNA. Hoppe Seylers Z. Physiol. Chem. 357, 313–314 (1976)Google Scholar
  67. Firtel, R.A., Cockburn, A., Frankel, G., Hershfield, V.: Structural organisation of the genome of Dictyostelium discoideum: Analysis by Ecl restriction endonuclease. J. Molec. Biol. 102, 831–852 (1976)PubMedGoogle Scholar
  68. Foster, T.J., Howe, T.G.B., Richmond, K.M.V.: Translocation of the tetracycline resistance determinant from R100-I to the Escherichia coli K12 chromosome. J. Bacteriol. 124, 1153–1158 (1975)PubMedGoogle Scholar
  69. Frame, R., Bishop, J.O.: The number of sex factors per chromosome in E. coli. Biochem. J. 121, 93–103 (1971)PubMedGoogle Scholar
  70. Frazer, A.C., Curtiss, R. III: Production, properties and utility of bacterial minicells. In: Current Topics in Microbiology and Immunology 69, pp. 1–84. Berlin: Springer-Verlag 1975Google Scholar
  71. Ganem, D., Nussbaum, A.L., Davoli, D., Fareed, G.C.: Propagation of a segment of bacteriophage A-DNA in Monkey cells after covalent linkage to a defective Simian virus 40 genome. Cell 7, 349–359 (1976)PubMedGoogle Scholar
  72. Garfin, D.E., Boyer, H.W., Goodman, H.W.: Sequences spanning the Ecl substrate site. Nucleic Acids Res. 2, 1851–1865 (1975)PubMedGoogle Scholar
  73. Gasson, M.J., Willetts, N.S.: Five control systems preventing transfer of Escherichia coli K12 sex factor F. J. Bacteriol. 122, 518–525 (1975)PubMedGoogle Scholar
  74. Gautier, F., Mayer, H., Goebel, W.: Cloning of Calf thymus satellite I DNA in Escherichia coli. Molec. Gen. Genet. 149, 23–31 (1976)PubMedGoogle Scholar
  75. Gilham, P. T.: Immobilized polynucleotides and nucleic acids. In: Advances in Experimental Medicine and Biology 42, pp. 173–185, Dunlap, R.B. (ed.). New York: Plenum Press 1974Google Scholar
  76. Glover, D.M., White, R.L., Finnegan, D.J., Hogness, D.S.: Characterisation of six cloned DNAs from Drosophila melanogaster, including one that contains the genes for NA. Cell 5, 149–157 (1975)PubMedGoogle Scholar
  77. Glover, D. M.: The construction and cloning of hybrid DNA molecules. In: New Techniques in Biophysics and Cell Biology. Vol. III. Pain. Smith (eds.). 1976 (in press)Google Scholar
  78. Goebel, W.: Replication of DNA of colicinogenic factor EI (Col) at restrictive temperature in a DNA replication mutant thermosensitive for DNA-polymerase III. Nature [New Biol] 237, 192 (1972)Google Scholar
  79. Goebel, W., Bonewald, R.: Class of small multicopy plasmids originating from the mutant antibiotic resistance factor Rldrd-19B2. J. Bacteriol. 123, 658–665 (1975)PubMedGoogle Scholar
  80. Gottesman, M.M., Rosner, J.L.: Acquisition of a determinant for chloramphenicol resistance by coliphage 1 Proc. Natl. Acad. Sci. U.S.A. 72, 5041–5045 (1975)Google Scholar
  81. Greene, P.J., Betlach, M.C., Boyer, H.W., Goodman, H.M.: The Ecl restriction endonuclease. In: Methods in Molecular Biology, Vol. VII, DNA replication, pp. 87–111. Wickner, R.B. (ed.). New York: Marcell Dekker 1975Google Scholar
  82. Grindley, N.D.F., Kelley, W.S.: Effects of different alleles of the E. colAl po gene on the replication of non-transferring plasmids. Molec. Gen. Genet. 143, 311–318 (1976)PubMedGoogle Scholar
  83. Gross, K., Probst, E., Schaffner, W., Bimstie I, M.: Molecular analysis of the histone gene cluster of Psammechinus miliaris. I. Fractionation and identification of five individual histone NAs. Cell 8, 455–469 (1976a)PubMedGoogle Scholar
  84. Gross, K., Schaffner, W., Telford, J., Birnstiel, M.: Molecular analysis of the histone gene cluster of Psammechinus miliaris. III. Polarity and asymmetry of the histone-coding sequences. Cell 8, 479–484 (1976 b)PubMedGoogle Scholar
  85. Grunstein, M., Hogness, D.S.: Colony hybridisation: A method for the isolation of cloned DNAs that contain a specific gene. Proc. Natl. Acad. Sci. U.S.A. 72, 3961–3965 (1975)PubMedGoogle Scholar
  86. Guild, W.R., Robison, M.: Evidence for message reading from a unique strand of pneumococcal DNA. Proc. Natl. Acad. Sci. U.S.A. 50, 106–112 (1963)PubMedGoogle Scholar
  87. Haggerty, D.M., Schleif, R.E.: Location in bacteriophage Lambda DNA of cleavage sites of the site-specific endonuclease from Bacillus amyloliquefaciens H. J. Virol. 18, 659–663 (1976)PubMedGoogle Scholar
  88. Hamer, D.H., Thomas, Jr., C.A.: Molecular cloning of DNA fragments produced by restriction endonucleases Sail and Baml. Proc. Natl. Acad. Sci. U.S.A. 73, 1537–1541 (1976)PubMedGoogle Scholar
  89. Hayashi, M.: A DNA-RNA complex as an intermediate of in vitro genetic transcription. Proc. Natl. Acad. Sci. U.S.A. 54, 1736–1743 (1965)PubMedGoogle Scholar
  90. Hedges, R.W., Jacob, A.E.: Transposition of ampicillin resistance from RP4 to other replicons. Molec. Gen. Genet. 132, 31–40 (1974)PubMedGoogle Scholar
  91. Hedges, R.W., Cresswell, J.M., Jacob, A.E.: A non-transmissible variant of RP4 suitable as cloning vehicle for genetic engineering. FEBS Lett. 61, 186–188 (1976)PubMedGoogle Scholar
  92. Hedgpeth, J., Goodman, H.M., Boyer, H.W.: DNA nucleotide sequence restricted by the RI endonuclease. Proc. Natl. Acad. Sci. U.S.A. 69, 3448–3452 (1972)PubMedGoogle Scholar
  93. Heffron, F., Rubens, C., Falkow, S.: Translocation of a plasmid DNA sequence which mediates ampicillin resistance: molecular nature and specificity of insertion. Proc. Natl. Acad. Sci. U.S.A. 72, 3623–3627 (1975)PubMedGoogle Scholar
  94. Helinski, D.R.: Plasmid determined resistance to antibiotics: molecular properties of R factors. Annu. Rev. Microbiol. 27, 437–469 (1973)PubMedGoogle Scholar
  95. Helinski, D.R., Herschman, H.R.: Effect of Rec mutations on the activity of colicinogenic factors. J. Bacteriol. 94, 700–706 (1967)PubMedGoogle Scholar
  96. Helinski, D.R., Clewell, D.B.: Circular DNA. Annu. Rev. Biochem. 40, 899–942 (1971)PubMedGoogle Scholar
  97. Helling, R.B., Goodman, H.M., Boyer, H.W.: Analysis of endonuclease R.Ecl fragments of DNA from Lambdoid bacteriophages and other viruses by agarose gel electrophoresis. J. Virol. 14, 1235–1244 (1974)PubMedGoogle Scholar
  98. Helmuth, R., Achtman, M.: Operon structure of DNA transfer cistrons on the F sex factor. Nature (Lond.) 257, 652–656 (1975)Google Scholar
  99. Hershfield, V., Boyer, H.W., Yanofsky, C., Lovett, M., Helinski, D.R.: Plasmid Col as a molecular vehicle for cloning and amplification of DNA. Proc. Natl. Acad. Sci. U.S.A. 71, 3455–3459 (1974)PubMedGoogle Scholar
  100. Hershfield, V., Boyer, H.W., Chow, L., Helinski, D.R.: Characterisation of a Mini-Col plasmid. J. Bacteriol. 126, 447–453 (1976)PubMedGoogle Scholar
  101. Heyneker, H.L., Shine, J., Goodman, H.W., Boyer, H.W., Rosenberg, J., Dickerson, R.E., Narang, S.A., Itakura, K., Lin, S.-Y., Riggs, A.D.: Synthetic lac operator DNA is functional in vivo. Nature (Lond.) 263, 748–752 (1976)Google Scholar
  102. Hickson, F.T., Roth, T.F., Helinski, D.R.: Circular DNA forms of a bacterial sex factor. Proc. Natl. Acad. Sci. U.S.A. 58, 1731–1738 (1967)PubMedGoogle Scholar
  103. Hill, C., Holland, LB.: Genetic basis of colicin E susceptibility in Escherichia coli. I. Isolation and properties of refractory mutants and the prelimary mapping of their mutations. J. Bacteriol. 94, 677–686 (1967)PubMedGoogle Scholar
  104. Hohn, B.: DNA as substrate for packaging into bacteriophage Lambda in vitro. J. Mol. Biol. 98, 93–106 (1975)PubMedGoogle Scholar
  105. Hopwood, D.A., Chater, K.F., Dowding, J.E., Vivian, A.: Advances in Streptomyces coelicolor genetics. Bacteriol. Rev. 37, 371–405 (1973)PubMedGoogle Scholar
  106. Horiuchi, K., Zinder, N.D.: Cleavage of the bacteriophage fl DNA by the restriction enzyme of Escherichia coli B. Proc. Natl. Acad. Sci. U.S.A. 69, 3220–3224 (1972)PubMedGoogle Scholar
  107. Hu, S., Ohtsubo, E., Davidson, N., Saedler , II.: Electron microscope heteroduplex studies of sequence relationships among bacterial plasmids: Identification and mapping of insertion sequences IS1 and IS2 in F and R plasmids. J. Bacteriol. 122, 764–775 (1975)PubMedGoogle Scholar
  108. Inselburg, J.: The isolation, mapping and examination of effects of T insertions in Col plasmids. J. Bacteriol. 129, 482–491 (1977)PubMedGoogle Scholar
  109. Jackson, D.A., Symons, R.H., Berg, P.: Biochemical method for inserting new genetic information into DNA of Simian virus 40: Circular SV40 DNA molecules containing Lambda phage genes and the galactose operon of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 69, 2904–2909 (1972)PubMedGoogle Scholar
  110. Jacob, A.E., Grinter, N.J.: Plasmid RP4 as a vector replicon in genetic engineering. Nature 255, 504–506 (1975)PubMedGoogle Scholar
  111. Jacob, A.E., Cresswell, J.M., Hedges, R.W., Coetzee, J.N., Beringer, J.E.: Properties of plasmids constructed by the in vitro insertion of DNA from Rhizobium leguminosarum or Proteus mirabilis into RP4. Molec. Gen. Genet. 147, 315–323 (1976)PubMedGoogle Scholar
  112. Jacob, F., Wollman, E.L.: Les épisomes, éléments génétiques ajoutés. C. R. Inst. Past. 247, 154–156 (1958)Google Scholar
  113. Jacob, F., Brenner, S., Cuzin, F.: On the regulation of DNA replication in bacteria. Cold Spring Harbor Symp. Quant. Biol. 28, 329–348 (1963)Google Scholar
  114. Karrer, K.M., Gall, J.G.: The macro nuclear ribosomal DNA of Tetrahymena pyriformis is a palindrome. J. molec. Biol. 104, 421–453 (1976)PubMedGoogle Scholar
  115. Kaufmann, G., Kallenba, N.R.: Determination of recognition sites of T4 RNA ligase on 3’-OH and 5’-P termini of polynucleotide chains. Nature (Lond.) 254, 452–454 (1975)Google Scholar
  116. Kedes, L.H., Chang, A.C.Y., Houseman, D., Cohen, S.N.: Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli. Nature (Lond.) 255, 533–538 (1975)Google Scholar
  117. Kelly, T.J., Smith, H.O.: A restriction nuclease from Haemophilus influenzae. II. Base sequence of the recognition site. J. Molec. Biol. 51, 393–409 (1970)PubMedGoogle Scholar
  118. Kingsbury, D.T., Helinski, D.R.: Temperature sensitive mutants for replication of plasmids in E. coli. I. Isolation and specificity of host and plasmid mutations. Genetics 74, 17–31 (1973)PubMedGoogle Scholar
  119. Kirby, R., Wright, L.F., Hopwood, D.A.: Plasmid determined antibiotic synthesis and resistance in Streptomyces coelicolor. Nature (Lond.) 254, 265–270 (1975)Google Scholar
  120. Kleppe, K., Van De Sande, J.H., Khorana, H.G.: Polynucleotide ligase-catalysed joining of deoxyribo-oligonucleotide templates and of ribo-oligonucleotides on deoxyribopolynu cleotide templates. Proc. Natl. Acad. Sci. U.S.A. 67, 68–73 (1970)PubMedGoogle Scholar
  121. Kolata, G.B.: Jumping genes: a common occurence in cells. Science 193, 392–394 (1976)PubMedGoogle Scholar
  122. Kopecko, D.J., Cohen, S.N.: Site-specific re-independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci. Proc. Natl. Acad. Sci. U.S.A. 72, 1373–1377 (1975)PubMedGoogle Scholar
  123. Kramer, R.A., Cameron, J.R., Davis, R.W.: Isolation of bacteriophage X containing yeast ribosomal RNA genes: screening by in situ RNA hybridisation to plaques. Cell 8, 227–232 (1976)PubMedGoogle Scholar
  124. Kretschmer, P.J., Chang, A.C.Y., Cohen, S.N.: Indirect selection of bacterial plasmids lacking identifiable phenotypic properties. J. Bacteriol. 124, 225–231 (1975)PubMedGoogle Scholar
  125. Landy, A., Ruedisueli, E., Robinson, L., Foeller, C., Ross, W.: Digestion of DNAs from bacteriophage T7, and 80 with site-specific nucleases from Haemophilus infulenzae strain Rc and strain Rd. Biochem. 13, 2134–2142 (1974)Google Scholar
  126. Last, J.A.: Purification and properties of RNA ligase from bacteriophage T4 infected E. coli. Fed. Proc. 34, 701 (1975)Google Scholar
  127. Lebowitz, P., Bloodgood, R.: Transcription of simian virus 40 DNA by Escherichia coli RNA polymerase: synthesis of a DNA-RNA hybrid and discrete RNAs under restrictive transcription conditions. J. Molec. Biol. 94, 183–201 (1975)PubMedGoogle Scholar
  128. Lederberg, E.M., Cohen, S.N.: Transformation of Salmonella typhimurium by plasmid DNA. J. Bacteriol. 119, 1072–1074 (1974)PubMedGoogle Scholar
  129. Lederberg, J.: Cell genetics and hereditary symbiosis. Physiol. Rev. 32, 403–430 (1952)PubMedGoogle Scholar
  130. Lewin, B.: Sequence organisation of eukaryotic DNA. Defining the unit of gene expression. Cell 1, 107–111 (1974)Google Scholar
  131. Lieberman, R.P., Oishi, M.: Formation of the re-re DN’ase by in vitro complementation and evidence concerning its subunit nature. Nature [New Biol.] 243, 75–77 (1973)Google Scholar
  132. Lindahl, L., Nomura, M.: Analysis and organisation of Escherichia coli ribosome and RNA polymerase genes, using biochemical methods. In: Control of Ribosome Synthesis. Ninth Alfred Benzon Symp. Kjeldgaard, N.O., Maaloe, O. (eds.). Copenhagen: Munksgaard 1976, pp. 206–217Google Scholar
  133. Lobban, P.E., Kaiser, A.D.: Enzymatic end to end joining of DNA molecules. J. Molec. Biol. 78, 453–471 (1973)PubMedGoogle Scholar
  134. Lovett, M.A., Helinski, D.R.: Relaxation complexes of plasmid DNA and protein. II. Characterisation of the proteins associated with the unrelaxed and relaxed complexes of plasmid Col. J. Biol. Chem. 250, 8790–8795 (1975)PubMedGoogle Scholar
  135. Lovett, M.A., Helinski, D.R.: Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F, Km plasmid. J. Bacteriol. 127, 982–987 (1976)PubMedGoogle Scholar
  136. Mandel, M., Higa, A.: Calcium-dependant bacteriophage DNA infection. J. Molec. Biol. 55, 159–162 (1970)Google Scholar
  137. Maniatis, T., Gek Kee, S., Efstratiadis, A., Kafatos, F.C.: Amplification and characterisation of a ß-globin gene synthesised in vitro. Cell 8, 163–182 (1976 a)PubMedGoogle Scholar
  138. Maniatis, T., Efstratiadis, A., Gek Kee, S., Kafatos, F.C.: In vitro synthesis and molecular cloning of eukaryotic structural genes. In: ICN-UCLA Symp. Molec. Cell. Biol, Vol. V. Nierlich, D.P., Rutter, W.J., Fox, C.F. (eds.). New York: Academic Press 1976bGoogle Scholar
  139. Marians, K.J., Wu, R., Stawinski, J., Hozumi, T., Narang, A.: Cloned synthetic lac operator DNA is biologically active. Nature (Lond.) 265, 744–748 (1976)Google Scholar
  140. Matsubara, K, Takagi, Y., Mukai, T.: In vitro construction of different oligomeric forms of X DNA and studies on their transforming activities. J. Virol. 16, 479–485 (1975)PubMedGoogle Scholar
  141. Matsubara, K: Genetic structure and regulation of a replicon of plasmid X. J. Molec. Biol. 102, 427–439 (1976)PubMedGoogle Scholar
  142. Meagher, R.B., Tait, R.D., Betlach, M., Boyer, H.W.: Protein expression in E. coli minieells by recombinant plasmids. Cell 10, 521–536 (1977)PubMedGoogle Scholar
  143. Mertz, J.E., Davis, R.W.: Cleavage of DNA by R1 restriction endonuclease generates cohesive ends. Proc. Natl. Acad. Sci. U.S.A. 69, 3370–3374 (1972)PubMedGoogle Scholar
  144. Meynell, E., Meynell, G.G., Datta, N.: Phylogenetic relationships of the drug resistance factors and other transmissible bacterial plasmids. Bacteriol. Rev. 52, 55–83 (1968)Google Scholar
  145. Morrow, J.F., Cohen, S.N., Chang, A.C.Y., Boyer, H.W., Goodman, H.M., Helling, R.B.: Replication and transcription of eukaryotic DNA in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 71, 1743–1747 (1974)PubMedGoogle Scholar
  146. Mukai, T., Matsubara, K, Takagi, Y.: In vitro construction of Col factor carrying genes for synthesis of guanine or thymine. Proc. Jpn. Acad. 51, 353–357 (1975)Google Scholar
  147. Mukai, T., Matsubara, K., Takagi, Y.: Cloning bacterial genes with plasmid X. Molec. Gen. Genet. 146, 269–274 (1976)PubMedGoogle Scholar
  148. Mulder, C., Delius, H.: Specificity of the break produced by restricting endonuclease R1 in simian virus 40 DNA as revealed by partial denaturation mapping. Proc. Natl. Acad. Sci. U.S.A. 69, 3515–3219 (1972)Google Scholar
  149. Müller, W., Gautier, F.: Interactions of heteroaromatic compounds with nucleic acids; A.T.-specific non-intercalating DNA ligands. Eur. J. Biochem. 54, 385–394 (1975)PubMedGoogle Scholar
  150. Murray, K, Murray, N.E., Brammar, W.J.: Restriction enzymes and the cloning of eukaryotic DNA. Proc. FEBS Meeting 10, 193–207 (1975)Google Scholar
  151. Nagel de Zwaig, R., Luria, S.E.: Genetics and physiology of colicin tolerant mutants of Escherichia coli. J. Bacteriol. 94, 1112–1123 (1967)Google Scholar
  152. Nagel de Zwaig, R., Luria, S.E.: New class of colicin tolerant mutants. J. Bacteriol. 99, 78–84 (1969)Google Scholar
  153. Nisioka, T., Mitani, M., Clowes, R.C.: Composite circular forms of R-factor deoxyribonucleic acid molecules. J. Bacteriol. 97, 376–385 (1969)PubMedGoogle Scholar
  154. Nomura, M., Witten, C.: Interactions of colicins with bacterial cells. III. Colicin-tolerant mutations in Escherichia coli. J. Bacteriol. 94, 1093–1111 (1967)PubMedGoogle Scholar
  155. Nordström, K, Ingram, C.L., Lundbäck, A.: Mutations in R factors of Escherichia coli causing an increased number of R-factor copies per chromosome. J. Bacteriol. 97, 375–385 (1972)Google Scholar
  156. Novick, R.P., Clowes, R.C., Cohen, S.N., Curtiss, R. III, Datta, N., Falkow, S.: Uniform nomenclature for bacterial plasmids: a proposal. Bacteriol. Rev. 40, 168–189 (1976)PubMedGoogle Scholar
  157. Ohtsubo, E., Soll, L., Deonier, R.C., Lee, H.J., Davidson, N.: Electron microscopic heteroduplex studies of sequence relations among plasmids of E. coli. VIII. The structure of phage f80d3 ilv su + l including the mapping of the ribosomal RNA. J. Molec. Biol. 89, 565–585 (1974)Google Scholar
  158. Oishi, M., Cosloy, S.D.: Specialised transformation in Escherichia coli Kl2. Nature (Lond.) 248, 112–116 (1974)Google Scholar
  159. Old, R.W., Murray, K., Roizes, G.: Recognition sequence of restriction endonuclease III from Haemophilus influenzae. J. Molec. Biol. 92, 331–339 (1975)PubMedGoogle Scholar
  160. Padmanaban, G., Hendler, F., Patzer, J., Ryan, R., Rabinowitz, M.: Translation of RNA that contains polyadenylate from yeast mitochondria in an Escherichia coli ribosomal system. Proc. Natl. Acad. Sci. U.S.A. 72, 4293–4297 (1975)PubMedGoogle Scholar
  161. Polisky, B., Greene, P., Garfin, D.E., Marthy, B.J., Goodman, H.M., Boyer, H.W.: Specificity of substrate recognition by the £cI restriction endonuclease. Proc. Natl. Acad. Sci. U.S.A. 72, 3310–3314 (1975)PubMedGoogle Scholar
  162. Pritchard, R.H., Barth, P.T., Collins, J.: Control of DNA synthesis in bacteria. Symp. Soc. Gen. Microbiol. 14, 263–297 (1969)Google Scholar
  163. Pritchard, R.H., Chandler, M.G., Collins, J.: Independence of F replication and chromosome replication in Escherichia coli. Molec. Gen. Genet. 138, 143–155 (1975)PubMedGoogle Scholar
  164. Pruss, G.J., Wang, J.C., Calendar, R.: In vitro packaging of covalently closed circular monomers of bacteriophage DNA. J. Molec. Biol. 98, 465–178 (1975)PubMedGoogle Scholar
  165. Rabbits, T.H.: Bacterial cloning of plasmids carrying copies of rabbit globin messenger RNA. Nature (Lond.) 260, 221–225 (1976)Google Scholar
  166. Radioff, R., Bauer, W., Vinograd, J.: A dye bouyant-density method for the detection and isolation of closed circular duplex DNA: Closed circular DNA in He La cells. Proc. Natl. Acad. Sci. U.S.A. 57, 1514–1521 (1967)Google Scholar
  167. Roberts, R.J.: Restriction enzymes. CRC Crit. Rev. Biochem. 4, 123–164 (1976)PubMedGoogle Scholar
  168. Roberts, R.J., Breitmeyer, J.B., Tabachnik, N.F., Myers, P.A.: A second specific nuclease from Haemophilus aegypticus. J. Molec. Biol. 91, 121–123 (1975)PubMedGoogle Scholar
  169. Rodriguez, R.L., Bolivar, F., Goodman, H.M., Boy er, H.W., Betlach, M.: Construction and characterisation of cloning vehicles. In: ICN-UCLA Symp. Molec. Cell. Biol. Vol. V. Nierlich, D.P., Rutter, W.J., Fox, C.F. (eds.). New York: Academic Press 1976Google Scholar
  170. Rougeon, F., Kourilsky, P., Mach, B.: Insertion of a rabbit ß-globin gene sequence into an E. coli plasmid. Nucleic Acids Res. 2, 2365–2378 (1975)PubMedGoogle Scholar
  171. Roy, P.H., Smith, H.O.: DNA methylases of Haemophilus influenzae Rd. II. Partial recognition site base sequences. J. Molec. Biol. 81, 445–459 (1973)PubMedGoogle Scholar
  172. Roychoudhury, R., Jay, E., Wu, R.: Terminal labelling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase. Nucleic Acids Res. 3, 101–116 (1976)PubMedGoogle Scholar
  173. Schaffner, W., Gross, K., Telford, J., Birnstiel, M.: Molecular analysis of the histone gene cluster of Psammechinus miliaris: II. The arrangement of the five histone-coding and spacer sequences. Cell 8, 471–478 (1976)PubMedGoogle Scholar
  174. Schrempf H., Bujard, H., Hopwood, D.A., Goebel, W.: Isolation of covalently closed circular DNA from Streptomyces coelicolor A3(2). J. Bacteriol. 121, 416–421 (1975)PubMedGoogle Scholar
  175. Sgaramella, V., Vand De Sande, J.H., Khorana, H.G.: Studies on polynucleotides. C.A. novel joining reaction catalysed by the T4-polynucleotide ligase. Proc. Natl. Acad. Sci. U.S.A. 67, 1468–1475 (1970)PubMedGoogle Scholar
  176. Sharp, P.A., Gallimore, P.H., Flint, S.J.: Mapping of the adenovirus 2 RNA sequences in lytically infected cells and transformed cell lines. Cold Spring Harbor Symp. Quant. Biol. 41, 457–473 (1975)Google Scholar
  177. Shiu Lee, A., Constantini, F., Britten, R.J., Davidson, E.H.: Initial studies of sea urchin DNA sequence organisation by molecular cloning. In: ICN-UCLA Symp. Molec. Cell Biol Vol. V. Nierlich, D.P., Rutter, W.J., Fox, C.F. (eds.). New York: Academic Press 1976Google Scholar
  178. Smith, D.I., Blattner, F.R., Davies, J.: The isolation and partial characterisation of a new restriction endonuclease from Providencia stuartii. Nucleic Acids Res. 3, 343–353 (1976)PubMedGoogle Scholar
  179. Smith, H.R., Humphreys, G.O., Willshaw, G.A., Anderson, E.S.: Characterisation of plasmids coding for the restriction endonuclease EcoR1. Molec. Gen. Genet. 143, 319–325 (1976)PubMedGoogle Scholar
  180. Smith, J.D., Arber, W., Kuhnlein, U.: Host specificity of DNA produced by Escherichia coli. XIV. The role of nucleotide methylation in vivo in B-specific modification. J. Molec. Biol. 63, 1–8 (1972)PubMedGoogle Scholar
  181. Snopek, T.J., Sugino, A., Agarwal, K.L., Cozzarelli, N.R.: Catalysis of DNA joining by bacteriophage T4 RNA ligase. Biochem. Biophys. Res. Commun. 68, 417–424 (1976)PubMedGoogle Scholar
  182. So, M., Gill, R., Falkow, S.: The generation of a Col-A cloning vehicle which allows detection of inserted DNA. Molec. Gen. Genet. 142, 239–249 (1975)PubMedGoogle Scholar
  183. Southern, E.M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Molec. Biol. 98, 503–517 (1975)PubMedGoogle Scholar
  184. Spizizen, J.: Transformation of biochemically deficient strains of B. subtilis by deoxyribonu-cleate. Proc. Natl. Acad. Sci. U.S.A. 44, 1072–1078 (1958)PubMedGoogle Scholar
  185. Staudenbauer, W.L.: Replication of Col in plasmolysed E. coli cells. Coupling of replication and RNA synthesis. Eur. J. Biochem. 58, 303–315 (1975)PubMedGoogle Scholar
  186. Staudenbauer, W.L.: Replication of small plasmids. Curr. Top. Microbiol. Immunol. (1978) (In preparation)Google Scholar
  187. Steitz, J.A., Jakes, K.: How ribosomes select initiator regions in NA: base pair formation between the 3’ terminus of 16S NA and the NA during initiation of protein synthesis in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 72, 4734–4738 (1975)PubMedGoogle Scholar
  188. Struhl, K., Cameron, J.R., Davis, R.W.: Functional genetic expression of eukaryotic DNA in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 73, 1471–1475 (1976)PubMedGoogle Scholar
  189. Stuy, J.H.: Transformability of Haemophilus influenzae. J. Gen. Microbiol. 29, 537–549 (1962)PubMedGoogle Scholar
  190. Syvanen, M.: Processing of bacteriophage Lambda DNA during its assembly into heads. J. Molec. Biol. 91, 165–174 (1975)PubMedGoogle Scholar
  191. Tabak, H.F., Borst, P.: Erroneous transcription of mitochondrial DNA by RNA polymerase from Escherichia coli. Biochim. Biophys. Acta 217, 356–364 (1970)PubMedGoogle Scholar
  192. Tait, R.C., Meagher, R.B., Goodman, H.W., Boyer, H.W.: The production of proteins by bacterial plasmids containing eukaryotic DNA fragments. In: ICN-UCLA Symp. Molec. Cell. Biol. Vol. V. Nierlich, D.P., Rutter, W.J., Fox, C.F. (eds.). New York: Academic Press 1976Google Scholar
  193. Tanaka, T., Weisblum, B.: Construction of a colicin El-R factor composite plasmid in vitro: Means for amplification of deoxyribonucleic acid. J. Bacteriol. 121, 354–362 (1975)PubMedGoogle Scholar
  194. Thomas, M., White, R.L., Davis, R.W.: Hybridisation of RNA to double-stranded DNA: formation of R-loops. Proc. Natl. Acad. Sci. U.S.A. 73, 2294–2298 (1976)PubMedGoogle Scholar
  195. Timmis, K., Cabello, F., Cohen, S.N.: Utilisation of two distinct modes of replication by a hybrid plasmid constructed in vitro from separate replicons. Proc. Natl. Acad. Sci. U.S.A. 71, 4556–4560 (1974)PubMedGoogle Scholar
  196. Timmis, K., Cabello, F., Cohen, S.N.: Cloning, isolation, and characterisation of replicating regions of complex plasmid genomes. Proc. Natl. Acad. Sci. U.S.A. 72, 2242–2246 (1975)PubMedGoogle Scholar
  197. Tyler, J., Sherratt, D.J.: Synthesis of E colicins in Escherichia coli. Molec. Gen. Genet. 140, 349–353 (1975)PubMedGoogle Scholar
  198. Uhlin, B.E., Nordstrom, K.: Plasmid incompatibility and control of replication: copy mutants of the R factor R1 in E. coli K12. J. Bacteriol. 124, 641–649 (1975)PubMedGoogle Scholar
  199. Venetianer, P., Leder, P.: Enzymatic synthesis of solid phasebound DNA sequences corresponding to specific mammalian genes. Proc. Natl. Acad. Sci. U.S.A. 71, 3892–3896 (1974)PubMedGoogle Scholar
  200. Wackernagel, W.: Genetic transformation in E. coli: the inhibitory role of the recBC DNAse. Biochem. Biophys. Res. Commun. 51, 306–311 (1973)PubMedGoogle Scholar
  201. Walker, G.C., Uhlenbec, O.C., Bedows, E., Gumport, R.I.: T4 induced RNA ligase joins single stranded oligoribonucleotides. Proc. Natl. Acad. Sci. U.S.A. 72, 122–126 (1975)PubMedGoogle Scholar
  202. Watanabe, T., Fukasawa, T., Takano, T.: Conversion of male bacteria of Escherichia coli K12 to resistance to f phages by infection with the episome “resistance transfer factor”. Virology 17, 217–219 (1962)PubMedGoogle Scholar
  203. Watanabe, T.: Infectious drug resistance in bacteria. Curr. Top. Microbiol. Immunol. 56, 43–98 (1971)PubMedGoogle Scholar
  204. Wensink, P.C., Finnegan, D.J., Donelson, J.E., Hogness, D.S.: A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster. Cell 3, 315–325 (1974)PubMedGoogle Scholar
  205. Wohlheiter, J.A., Falkow, S., Citarella, R.V., Baron, L.S.: Characterisation of DNA from a proteus strain harboring an episome. J. Molec. Biol. 9, 576–588 (1964)Google Scholar
  206. Wood, K.O., Lee, J.C.: Integration of synthetic globin genes into an E. coli plasmid. Nucleic Acids Res. 3, 1961–1971 (1976)PubMedGoogle Scholar
  207. Yang, H-L., Zubay, G., Levy, S.B.: Synthesis of an R plasmid protein associated with tetracycline resistance is negatively regulated. Proc. Natl. Acad. Sci. U.S.A. 73, 1509–1512 (1976)PubMedGoogle Scholar
  208. Zaritsky, A., Pritchard, R.H.: Replication time of the chromosome in thymineless mutants of Escherichia coli. J. Molec. Biol. 60, 65–74 (1971)PubMedGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 1977

Authors and Affiliations

  • John Collins
    • 1
  1. 1.Gesellschaft für Biotechnologische Forschung mbHBraunschweig-StöckheimFederal Republic of Germany

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