Rat leukemia cells were harvested from the peritoneal cavity, suspended in medium (BME, supplemented with 20% fetal calf serum, 0.03% glutamine and Hepes in a 0.01 M concentration), and immediately introduced into assembled culture chambers [Rose, 1954; Dvorak and Stotler, 1971]. The chambers were then transfered to 37° C.
KeywordsCulture Chamber Perfusion Flow Perfusion Fixation Ficoll Gradient Amyl Acetate
Unable to display preview. Download preview PDF.