Rat leukemia cells were harvested from the peritoneal cavity, suspended in medium (BME, supplemented with 20% fetal calf serum, 0.03% glutamine and Hepes in a 0.01 M concentration), and immediately introduced into assembled culture chambers [Rose, 1954; Dvorak and Stotler, 1971]. The chambers were then transfered to 37° C.
KeywordsLeukemia Dehydration Glutamine Hepes Paraformaldehyde
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