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Methodology

  • Heidi Felix
  • Gisela Haemmerli
  • Peter Sträuli

Abstract

Rat leukemia cells were harvested from the peritoneal cavity, suspended in medium (BME, supplemented with 20% fetal calf serum, 0.03% glutamine and Hepes in a 0.01 M concentration), and immediately introduced into assembled culture chambers [Rose, 1954; Dvorak and Stotler, 1971]. The chambers were then transfered to 37° C.

Keywords

Culture Chamber Perfusion Flow Perfusion Fixation Ficoll Gradient Amyl Acetate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin · Heidelberg 1978

Authors and Affiliations

  • Heidi Felix
    • 1
  • Gisela Haemmerli
    • 1
  • Peter Sträuli
    • 1
  1. 1.Division of Cancer Research, Institute of PathologyUniversity of ZürichZürichSwitzerland

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