Regeneration of Virus-free Potato from Tissue Culture

  • P. J. Wang
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


Shoot tip and internodal explants from healthy and potato virus X (PVX) infected potato stocks were cultured in vitro. The potential of calli initiation and their rate of growth prior to subculture were considerably lower in the expiant from PVX infected stock. After subculture fast growing calli were induced regardless of the tissue origin. These calli regenerated shoots on regeneration medium. Adventitious shoots also arose directly from the vascular bundle of internodal explants. These adventitious shoots subsequently developed into complete plants. Approximately 40% of the plants derived from shoot tip calli of PVX infected stock were PVX free, while only 8% of those derived from internodal calli were PVX free. No virus free plants were detected among plants differentiated directly from internodal vascular tissue.


Vascular Bundle Tobacco Mosaic Virus Potato Virus Malachite Green Adventitious Shoot 
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  1. 1.
    Blakely, L.M., Steward, F.C.: Growth and organized development of cultured cells. VII. Cellular variation. Am. J. Botany 51, 809–820 (1964)CrossRefGoogle Scholar
  2. 2.
    Chandra, N., Hildebrandt, A.C.: Differentiation of plants from TWV inclusion-bearing and inoculum-free tobacco cells. Virology 31, 414–421 (1967)PubMedCrossRefGoogle Scholar
  3. 3.
    Fox, J.E.: Growth factor requirements and chromosome number in tobacco tissue cultures. Physiol. Plantarum 16, 793–803 (1963)CrossRefGoogle Scholar
  4. 4.
    Gregorini, G., Lorenzi, R.: Meristem-tip culture of potato plants as a method of improving productivity. Potato Res. 17, 24–33 (1974)CrossRefGoogle Scholar
  5. 5.
    Hakkaart, F.A., Ouak, F.: Effect of heat treatment of young plants on freeing Chrysanthemums from virus B by means of meristem culture. Neth. J. Path. 70, 154–157 (1964)CrossRefGoogle Scholar
  6. 6.
    Hansen, A.J., Hildebrandt, A.C.: The distribution of tobacco mosaic virus in plant callus culture. Virology 28, 15–21 (1966)PubMedCrossRefGoogle Scholar
  7. 7.
    Kassanis, B.: Potato tubers freed from leaf-roll virus by heat. Nature (London) 164, 881 (1949)CrossRefGoogle Scholar
  8. 8.
    Kassanis, B.: The multiplication of tobacco mosaic virus in cultures of tumerous tobacco tissues. Virology 4, 5–13 (1957a)PubMedCrossRefGoogle Scholar
  9. 9.
    Kassanis, B.: The use of tissue cultures to produce virus-free clones from infected potato varieties. Ann. Appl. Biol. 45, 422–427 (1957b)CrossRefGoogle Scholar
  10. 10.
    Kassanis, B., Varma, A.: The production of virus-free clones of some British potato varieties. Ann. Appl. Biol. 59, 447–450 (1967)CrossRefGoogle Scholar
  11. 11.
    Macdonald, D.M.: Heat treatment and meristem culture as a means of freeing potato varieties from viruses X and S. Potato Res. 16, 263–269 (1973)CrossRefGoogle Scholar
  12. 12.
    Morel, G., Martin, C.: Guerison des pommes de terre atteintes de maladies avirus. C.R. Acad. Agr. Fr. 41, 472–475 (1955)Google Scholar
  13. 13.
    Morel, G., Martin, C, Muller, F.: La guerison des pommes de terre atteintes de maladies a virus. Ann. Physiol. Veg. 10, 113–139 (1968)Google Scholar
  14. 14.
    Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum 15, 473–497 (1962)CrossRefGoogle Scholar
  15. 15.
    Mellor, F.C., Stace-Smith, R.: Eradication of potato virus X by thermotherapy. Phytopathology 57, 674–678 (1967)PubMedGoogle Scholar
  16. 16.
    Milo, G.E., Scrivastava, S.B.I.: Effect of cytokinins on tobacco mosaic virus production in local lesion and systemic host. Virology 38., 26–31 (1969a)PubMedCrossRefGoogle Scholar
  17. 17.
    Milo, G.E., Scrivastava, S.B.I.: Effect of cytokinins on tobacco mosaic virus production in tobacco pith tissue culture. Virology 39, 621–623 (1969b)PubMedCrossRefGoogle Scholar
  18. 18.
    Norris, D.O.: Development of virus-free stock of Green Mountain potato by treatment with malachite Green. Australian J. Agr. Res. 5, 658–663 (1954)CrossRefGoogle Scholar
  19. 19.
    Pillai, S.K., Hildebrandt, A.C.: Geranium plants differentiated in vitro from stem tip and callus culture. Pl. Dis. Reptr. 52, 600–601 (1968)Google Scholar
  20. 20.
    Quak, F.: Heat treatment and substances inhibiting virus multiplication, in meristem culture to obtain virus-free plants. Adv. Hort. Sci. Appl. 1, 144–148 (1961)Google Scholar
  21. 21.
    Simonsen, J., Hildebrandt, A.C.: In vitro growth and differentiation of gladiolus plants from callus cultures. Can. J. Botany 49, 1817–1819 (1971)CrossRefGoogle Scholar
  22. 22.
    Sip, V.: Eradication of potato viruses A and S by thermotherapy and sprout tip culture. Potato Res. 15, 270–273 (1972)CrossRefGoogle Scholar
  23. 23.
    Stace-Smith, R., Mellor, F.C.: Eradication of potato viruses X and S by thermotherapy and axillary bud culture. Phytopathology 58, 199–203 (1968)Google Scholar
  24. 24.
    Thomson, A.D.: Heat treatment and tissue culture as a means of freeing potatoes from virus Y. Nature (London) 177, 709 (1956)CrossRefGoogle Scholar
  25. 25.
    Wang, P.J., Loo, Y.C.: Elimination of potato viruses by means of shoot tip culture. Chinese Biosci. 2 19–24 (1973)Google Scholar
  26. 26.
    Wang, P.J., Huang, L.C.: Callus cultures from potato tissues and the exclusion of potato virus X from plants regenerated from shoot tips. Can. J. Botany 53, 2565–2567 (1975)CrossRefGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 1977

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  • P. J. Wang

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