Regeneration of Virus-free Potato from Tissue Culture
Shoot tip and internodal explants from healthy and potato virus X (PVX) infected potato stocks were cultured in vitro. The potential of calli initiation and their rate of growth prior to subculture were considerably lower in the expiant from PVX infected stock. After subculture fast growing calli were induced regardless of the tissue origin. These calli regenerated shoots on regeneration medium. Adventitious shoots also arose directly from the vascular bundle of internodal explants. These adventitious shoots subsequently developed into complete plants. Approximately 40% of the plants derived from shoot tip calli of PVX infected stock were PVX free, while only 8% of those derived from internodal calli were PVX free. No virus free plants were detected among plants differentiated directly from internodal vascular tissue.
KeywordsSucrose Agar Paraffin Hematoxylin Milo
Unable to display preview. Download preview PDF.
- 12.Morel, G., Martin, C.: Guerison des pommes de terre atteintes de maladies avirus. C.R. Acad. Agr. Fr. 41, 472–475 (1955)Google Scholar
- 13.Morel, G., Martin, C, Muller, F.: La guerison des pommes de terre atteintes de maladies a virus. Ann. Physiol. Veg. 10, 113–139 (1968)Google Scholar
- 19.Pillai, S.K., Hildebrandt, A.C.: Geranium plants differentiated in vitro from stem tip and callus culture. Pl. Dis. Reptr. 52, 600–601 (1968)Google Scholar
- 20.Quak, F.: Heat treatment and substances inhibiting virus multiplication, in meristem culture to obtain virus-free plants. Adv. Hort. Sci. Appl. 1, 144–148 (1961)Google Scholar
- 23.Stace-Smith, R., Mellor, F.C.: Eradication of potato viruses X and S by thermotherapy and axillary bud culture. Phytopathology 58, 199–203 (1968)Google Scholar
- 25.Wang, P.J., Loo, Y.C.: Elimination of potato viruses by means of shoot tip culture. Chinese Biosci. 2 19–24 (1973)Google Scholar