Regeneration of Virus-free Potato from Tissue Culture
Shoot tip and internodal explants from healthy and potato virus X (PVX) infected potato stocks were cultured in vitro. The potential of calli initiation and their rate of growth prior to subculture were considerably lower in the expiant from PVX infected stock. After subculture fast growing calli were induced regardless of the tissue origin. These calli regenerated shoots on regeneration medium. Adventitious shoots also arose directly from the vascular bundle of internodal explants. These adventitious shoots subsequently developed into complete plants. Approximately 40% of the plants derived from shoot tip calli of PVX infected stock were PVX free, while only 8% of those derived from internodal calli were PVX free. No virus free plants were detected among plants differentiated directly from internodal vascular tissue.
KeywordsVascular Bundle Tobacco Mosaic Virus Potato Virus Malachite Green Adventitious Shoot
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