Enhanced Platelet Aggregation as a Risk Factor for Progress and Complications of Vascular Disease. New Findings with a Platelet Aggregation Test (PAT III) and on the Dependence of Different Aggregation Tests on Morphologic Platelet Changes
Our interest in the measurement of enhanced platelet aggregation resulted from the idea that it may be a risk factor for the development of thrombosis and for the progression of atherosclerosis. For the development and for the control of antiaggregating drugs reliable methods for the measurement of platelet aggregation also are needed, which reflect at least partially in vivo conditions. With different methods, contradictory results have been published on this subject. We present some new findings on the interdependence of different aggregation tests and on the changes of induced and “spontaneous” aggregation found parallel to morphologic platelet changes which generally occur in platelet-rich plasma. Using platelet aggregation test I (PAT I), in which platelet aggregates are evaluated after 10 min rotation at 37 °C in a glass flask we demonstrated enhanced platelet aggregation in patients with diabetes, venous thrombosis, recent myocardial infarction, and progressive atherosclerosis.
KeywordsCitrate Adrenaline Thrombin Tated Hyde
Unable to display preview. Download preview PDF.
- Born, G.V.R.: Quantitative investigations into the aggregation of blood platelets. J. Physiol. (Lond.) 162, 67 (1962)Google Scholar
- Breddin, K.: Die Thrombozytenfunktion bei hämorrhagischen Diathesen, Thrombosen und Gefäßkrankheiten. Thromb. Diath. haemorrh. Suppl. 27 (1968)Google Scholar
- Breddin, K., Grun, H., Krzywanek, H.J., Schremmer, W.P.: On the measurement of spontaneous platelet aggregation. The platelet aggregation test III. Methods and first results. In preparationGoogle Scholar
- Breddin, K., Ludwig, H., Ziemen, M., Bauer, M., Schaudinn, I.: Time dependent changes of ADP-collagen and epinephrine-induced in “spontaneous” platelet aggregation and morphologic platelet changes. In preparationGoogle Scholar
- Fyfe, T., Glasg, M.B., Hamilton, E.: Effect of variation of the interval between venipuncture and measurement of platelet adhesiveness by the Payling Wright method. The Lancet 542, 543 (1967)Google Scholar
- Krzywanek, H.J., Breddin, K.: Der Plättchenaggregationstest (PAT). Med. Lab. (Stuttg.) 15, 103 (1972)Google Scholar
- Leuschner, M., Breddin, K.: Electronmicroscopic investigations. In preparation O’Brien, J.R.: Some possible enzyme systems involved in platelets aggregation. J. Atheroscl. Res. 3, 262 (1961)Google Scholar
- Reuter, H., Podolsak, W., Hagen, T., Linker, H., Stroder, J., Gross, R.: Investigations on the time dependance of platelet functions. J. gen. meth. Invest. Thromb. Res. 3, 307 (1973)Google Scholar