Abstract
A morphological examination of cerebrospinal fluid (CSF) cells is useful in the clinical diagnosis of most of the inflammatory and of some neoplastic and vascular diseases of the central nervous system (15). Various sedimentation chambers (15), a cytocentrifuge (4), membrane filters (5), and other techniques (7, 14) may be used for cell preparation. Besides routine stains, cytochemical reactions are being used in CSF cytological examination, especially in the diagnosis of meningeal leukemia (11). An increased RNA synthesis of tumor cells in CSF has been demonstrated by acridine orange fluorochromation (9, 14). Mainly for an improved demonstration of tumor cells in CSF diagnosis, several authors have suggested additional cytological examinations, which require advanced techniques of cell preparation: electron microscopy, cell cultures, and 3H-thymidine autoradiography (7, 8, 9, 10, 13).
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Dommasch, D., Gaab, M. (1977). Advanced Cytological Techniques in the Examination of Human Cerebrospinal Fluid. In: Wüllenweber, R., Brock, M., Hamer, J., Klinger, M., Spoerri, O. (eds) Lumbar Disc Adult Hydrocephalus. Advances in Neurosurgery, vol 4. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66578-3_43
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DOI: https://doi.org/10.1007/978-3-642-66578-3_43
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