Skip to main content

The Proton Pump of the Mitochondrial Respiratory Chain

  • Conference paper
Biochemistry of Membrane Transport

Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

Abstract

Fifteen years after Mitchell (1961) first proposed the chemiosmotic hypothesis of oxidative phosphorylation, it has been experimentally established that redox chains and ATPase complexes of energy-transducing membranes directly convert metabolic energy into a transmembrane thermodynamic potential difference of protons (for review, see: Mitchell, 1972; Papa, 1976). The mechanism of the redox and the hydrolytic pumps and their role in ATP formation remain, however, yet to be understood (Papa, 1976).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Albracht, S.P.J., Slater, E.C.: EPR studies at 20°K on the mitochondrial respiratory chain. Biochim. Biophys. Acta 245, 503–507 (1971)

    Article  PubMed  CAS  Google Scholar 

  • Baum, H., Rieske, J.S., Silman, H.I., Lipton, S.H.: On the mechanism of electron transfer in complex III of the electron transfer chain. Proc. Natl Acad. Sci. U.S. 57, 798–805 (1967)

    Article  CAS  Google Scholar 

  • Brand, M.D., Reynafarje, B., Lehninger, A.L.: The stoichiometry, relationship between energy-dependent proton ejection and electron transport in mitochondria. Proc. Natl. Acad. Sci. U.S. 73, 1–18 (1976)

    Article  Google Scholar 

  • Chance, B.: The nature of electron transfer and energy coupling reactions. FEBS Lett. 23, 3–20 (1972)

    Article  PubMed  CAS  Google Scholar 

  • Clark, W.M.: Oxidation-Reduction Potentials of Organic Systems. Baltimore Md.: Waverley Press, 1960

    Google Scholar 

  • Das Gupta, U., Rieske, J.S.: Identification of a protein component of the antimycin-binding site of the respiratory chain by photoaffinity labeling. Biochem. Biophys. Res. Commun. 54, 1247–1254 (1973)

    Article  PubMed  Google Scholar 

  • Douglas, M.G., Cockrell, R.S.: Mitochondrial cation-hydrogen ion exchange -Sodium selective transport by mitochondria and submitochondrial particles J. Biol. Chem. 249, 5464–5471 (1974)

    PubMed  CAS  Google Scholar 

  • Dutton, P.L., Wilson, D.F.: Redox potentiometry in mitochondrial and photosynthetic bioenergetics. Biochim. Biophys. Acta 346, 165–212 (1974)

    PubMed  CAS  Google Scholar 

  • Gellerfors, P., Nelson, B.D.: Analysis of the peptide composition of purified beef-heart complex III by dodecylsulfate electrophoresis. Europ. J. Biochem. 52, 433–443 (1975)

    Article  PubMed  CAS  Google Scholar 

  • Green, D.E., Järnefelt, J., Tisdale, H.D.: Studies on the electron transport system. XIV. The isolation and properties of soluble cytochromec. Biochim. Biophys. Acta 31, 34–46 (1959)

    Article  PubMed  CAS  Google Scholar 

  • Guerrieri, F., Nelson, B.D.: Studies on the characteristics of a proton pump in phospholipid vesicles inlayed with purified complex III from beef-heart mitochondria. FEBS Lett. 54, 339–342 (1975)

    Article  PubMed  CAS  Google Scholar 

  • Hinkle, P.C., Kim, J.J.: Chemiosmotic coupling in cytochrome oxidase vesicles. In: Mechanisms in Bioenergetics. Azzone, G.F., Ernster, L., Papa, S., Quagliariello, E., Siliprandi, N. (eds.). London: Academic Press, 1973; pp. 53–60

    Google Scholar 

  • Jencks, W.P.: Catalysts in Chemistry and Enzymology. New York: McGraw Hill, 1969, pp. 243–281

    Google Scholar 

  • Kilmartin, J.V., Rossi-Bernardi, L.: Interaction of hemoglobin with hydrogen ions, carbon dioxide and organic phosphates. Physiol. Rev. 53, 836–889 (1973)

    PubMed  CAS  Google Scholar 

  • Klingenberg, M.: The respiratory chain. In: Biological Oxidation. Singer, P.T. (ed.). New York: Interscience (1968), pp. 1–49

    Google Scholar 

  • Lawford, H.G., Garland, P.B.: Proton translocation coupled to quinol oxidation in ox-heart mitochondria. Biochem J. 136, 711–720 (1973)

    PubMed  CAS  Google Scholar 

  • Mitchell, P.: Coupling of phosphorylation to electron and hydrogen transfer by a chemiosmotic type of mechanism. Nature (London) 191, 144–148 (1961)

    Article  CAS  Google Scholar 

  • Mitchell, P.: Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Biol. Rev. 41, 445 (1966)

    Article  PubMed  CAS  Google Scholar 

  • Mitchell, P.: Structural and functional organisation of energy-transducing membranes and their ion-conducting properties. In: Mitochondria/Biomembranes. In: Proc. 8th FEBS Meeting, Amsterdam. Van Den Bergh, S.G., Borst, P., Van Deenen, L.L.M., Reimersma, J.C., Slater, E.C., Tager, J.M. (eds.). Amsterdam: North Holland-American Elsevier, 1972, Vol. XXVIII, pp. 353–370

    Google Scholar 

  • Mitchell, P.: Protonmotive redox mechanism of the cytochrome b-c1 complex in the respiratory chain: Protonmotive ubiquinone cycle. FEBS Lett. 56, 1–6 (1975a)

    Article  PubMed  CAS  Google Scholar 

  • Mitchell, P.: Protonmotive function of cytochrome systems. In: Electron Transfer Chains and Oxidative Phosphorylation. Quagliariello, E., Papa, S., Palmieri, F., Slater, E.C., Siliprandi, N. (eds.). Amsterdam: North Holland, Publ. Co., 1975b, pp. 305–327

    Google Scholar 

  • Mitchell, P., Moyle, J.: Respiration-driven proton translocation in rat liver mitochondria. Biochem. J. 105, 1147–1162 (1967)

    PubMed  CAS  Google Scholar 

  • Mitchell, P., Moyle, J.: The intrinsic anisotropy of the cytochrome oxidase region of the mitochondrial respiratory chain and the consequent vectorial property of respiration. In: Electron Transport and Energy Conservation. Tager, J.M., Papa, S., Quagliariello, E., Slater, E.C. (eds.). Bari: Adriatica Editrice, 1970, pp. 575–587

    Google Scholar 

  • Nicholls, P., Chance, B.: Cytochromec oxidase. In: Molecular Mechanisms of Oxygen Activation. Hayaishi, O. (ed.). New York: Academic Press, 1974, pp. 479–534

    Google Scholar 

  • Nishibayashi-Yamashita, H., Cunningham, C., Racker, E.: Resolution and Reconstitution of the Mitochondrial Electron Transport System. III. Order of reconstitution and requirement for a new factor for respiration. J. Biol. Chem. 247, 698–704 (1972)

    CAS  Google Scholar 

  • Ohnishi, T., Wilson, D.F., Asakura, T., Chance, B.: Studies on iron-sulfur proteins in the site I region of the respiratory chain in pigeon heart mitochondria and submitochondrial particles. Biochem. Biophys. Res. Commun. 46, 1631–1638 (1972)

    Article  PubMed  CAS  Google Scholar 

  • Papa, S.: Coupling mechanism in ion translocation across the inner mitochondrial membrane. In: Energy Transduction in Respiration and Photosynthesis. Quagliariello, E., Papa, S., Rossi, C.S. (eds.). Bari: Adriatica Editrice, 1971, pp. 173–203

    Google Scholar 

  • Papa, S., Proton translocation reactions in the respiratory chains. Biochim. Biophys. Acta 456, 39–84 (1976)

    PubMed  CAS  Google Scholar 

  • Papa, S., Guerrieri, F., Lorusso, M., Simone, S.: Proton translocation and energy transduction in mitochondria. Biochimie 55, 703–716 (1973)

    Article  PubMed  CAS  Google Scholar 

  • Papa, S., Guerrieri, F., Lorusso, M.: Mechanism of respiration-driven proton translocation in the inner mitochondrial membrane. Analysis of proton translocation associated to oxido-reductions of the oxygen-terminal respiratory carriers. Biochim. Biophys. Acta 357, 181–192 (1974a)

    Article  PubMed  CAS  Google Scholar 

  • Papa, S., Guerrieri, F., Lorusso, M.: The role of respiratory carriers in the mitochondrial proton pump. In: Membrane Proteins in Transport and Phosphorylation. Azzone, G.F., Klingenberg, M.E., Quagliariello, E., Siliprandi, N. (eds.). Amsterdam: North Holland, Publ. Co., 1974b, pp. 177–186

    Google Scholar 

  • Papa, S., Guerrieri, F., Lorusso, M.: On the mechanism of the mitochondrial proton pump. In: Dynamics of Energy-Transducing Membranes. Ernster, L., Estabrock, R.W., Slater, E.C. (eds.). Amsterdam: Elsevier, 1974c, pp. 417–432

    Google Scholar 

  • Papa, S., Guerrieri, F., Capuano, F., Izzo, G., Boffoli, D.: On the stoichiometry of the mitochondrial redox-proton pump. 1976, to be submitted

    Google Scholar 

  • Papa, S., Lorusso, M., Guerrieri, F.: Mechanism of respiration-driven proton translocation in the inner mitochondrial membrane. Analysis of proton translocation associated with oxidation of endogenous ubiquinol. Biochim. Biophys. Acta 387, 425–440 (1975a)

    Article  PubMed  CAS  Google Scholar 

  • Papa, S., Lorusso, M., Guerrieri, F. Izzo, G.: On the mechanism of electron flow and proton translocation in the ubiquinone-cytochromec span of the respiratory chain. In: Electron Transfer Chains and Oxidative Phosphorylation. Quagliariello, E., Papa, S., Palmieri, F., Slater, E.C., Siliprandi, N. (eds.). Amsterdam: Elsevier, 1975b, pp. 317–327

    Google Scholar 

  • Pettigrew, G.W., Meyer, T.E., Bartsch, R.G., Kamen, M.D.: pH dependence of the oxidation-reduction potential of cytochrome C2. Biochim. Biophys. Acta 430, 197–208 (1975)

    Google Scholar 

  • Rodkey, F.L., Ball, E.G.: Oxidation-reduction potentials of the cytochrome c system. Arch. Biochem. 12, 17–28 (1949)

    Google Scholar 

  • Seki, S., Oda, T.: Studies on cytochrome oxidase. II Ultrastructure of cytochrome oxidase. Arch. Biochem. Biophys. 138, 122–134 (1970)

    Article  PubMed  CAS  Google Scholar 

  • Slater, E.C.: Electron transfer and energy conservation. In: Dynamics of Energy-Transducing Membranes. Ernster, L., Estabrock, R.W., Slater, E.C. (eds.). Amsterdam: Elsevier 1974, pp. 1–20

    Google Scholar 

  • Straub, J.P., Colpa-Boonstra, J.P.: The effect of pH on the oxidation-reduction potential of cytochrome b in heart muscle preparations. Biochim. Biophys. Acta 60, 650–652 (1962)

    Article  PubMed  CAS  Google Scholar 

  • Urban, P.F., Klingenberg, M.: On the redox potentials of ubiquinone and cytochrome b in the respiratory chain. Europ. J. Biochem. 9, 519–525 (1969)

    Article  PubMed  CAS  Google Scholar 

  • Vanderkooi, G., Senior, A.E., Capaldi, R.A., Hayashi, H.: Biological membrane structure. III. The Lattice structure of membranous cytochrome oxidase. Biochim. Biophys. Acta 274, 38–48 (1972)

    Article  PubMed  CAS  Google Scholar 

  • Vernon, L.P., Kamen, M.D.: Hematin compounds in photosynthetic bacteria. J. Biol. Chem. 211, 643–675 (1954)

    PubMed  CAS  Google Scholar 

  • Wikström, M.K.F.: Energy linked change in the redox state and absorption spectrum of cytochrome a in situ. Biochim. Biophys. Acta 283, 385–390 (1972)

    Article  PubMed  Google Scholar 

  • Wyman, J.: Regulation in macromolecules as illustrated by haemoglobin. Quart. Rev. Biophys. 1, 35–81 (1968)

    Article  CAS  Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1977 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Papa, S., Guerrieri, F., Lorusso, M., Izzo, G., Boffoli, D., Capuano, F. (1977). The Proton Pump of the Mitochondrial Respiratory Chain. In: Semenza, G., Carafoli, E. (eds) Biochemistry of Membrane Transport. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66564-6_38

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-66564-6_38

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-66566-0

  • Online ISBN: 978-3-642-66564-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics