Skip to main content
SpringerLink
Log in
Book cover

Dynamics of Brain Edema pp 32–37Cite as

Determination of Cerebral Edema by Quantitative Morphometry

  • Conference paper

Summary

Occurrence of diffuse cerebral edema is well documented in both human and experimental hypertensive encephalopathy. Another consistent observation in acute experimental hypertension is an alteration of blood-brain barrier (BBB) permeability in multifocal cerebral cortical areas as evidenced by extravasation of trypan blue. The relationship of these areas to the occurrence of edema has not been previously studied. In this work the morphology of areas showing alterations of BBB permeability was studied and compared to that of nonpermeable areas and in normotensive controls.

Acute hypertension was induced in rats by i.v. infusion of angiotensin and permeability alterations identified by i.v. trypan blue. After carefully controlled fixation and embedding, astrocytic perivascular foot processes were measured from electron micrographs taken under standard conditions.

The surface areas (and hence the volume) of astrocytic foot processes were significantly greater in the permeable areas of the hypertensive animals than in the adjacent nonpermeable areas from the same animals and from comparable areas in normotensive animals.

It was concluded that the edema was confined to the permeable areas in this model of acute severe hypertension.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alajouanine, Th.: Discussion on cerebral edema. Proc. roy. Soc. Med. 40, 686–689 (1946–47)

    Google Scholar 

  2. Blackfan, K.D.: Acute nephritis in children, with special reference to the treatment of uremia. Bull. Johns Hopk. Hosp. 39, 69–90 (1926)

    Google Scholar 

  3. Byrom, F.B.: The pathogenesis of hypertensive encephalopathy and its relation to the malignant phase of hypertension. Lancet 261 (I): 201–211 (1954)

    Article  Google Scholar 

  4. Dinsdale, H.B., Robertson, D.M., Chiang, T.Y., Murkherjee, S.K.: Hypertensive cerebral microinfarction and cerebrovascular reactivity. Europ. Neurol. 6, 29–33 (1971/72)

    Google Scholar 

  5. Eto, T., Omae, T., Yamamoto, T.: An electron microscope study of hypertensive encephalopathy in the rat with renal hypertension. Arch. histol. jap. 33, 133–134 (1971)

    PubMed  CAS  Google Scholar 

  6. Finnerty, F.A.: Hypertensive encephalopathy. Amer. Heart J. 75, 559–563 (1968)

    Article  PubMed  Google Scholar 

  7. Johansson, B., Li, Choh-Luh, Olsson, Y., Klatzo, I.: The effect of acute arterial hypertension on the blood-brain barrier to protein tracers. Acta neuropath. (Berl.) 16, 117–124 (1970)

    CAS  Google Scholar 

  8. Karnovsky, M.J.: The ultrastructural basis of capillary permeability studied with peroxidase as a tracer. J. Cell. Biol. 35, 213 (1967)

    Article  PubMed  CAS  Google Scholar 

  9. Kung, P.C., Lee, J.C., Bakay, L.: Electron microscopic study of experimental acute hypertensive encephalopathy. Acta neuropath. (Berl.) 10, 263–272 (1968)

    CAS  Google Scholar 

  10. Meyer, J.S., Waltz, A.G., Gotoh, F.: Pathogenesis of cerebral vasospasm in hypertensive encephalopathy. Neurology (Minneap.) 10, 735–744 (1960)

    Google Scholar 

  11. Olsson, Y., Hossmann, K.A.: Fine structural localization of exudated protein tracers in the brain. Acta neuropath. (Berl.) 16, 103–116 (1970)

    CAS  Google Scholar 

  12. Oppenheimer, B.S., Fishberg, A.M.: Hypertensive encephalopathy. Arch. intern. Med. 41, 264–278 (1928)

    Google Scholar 

  13. Rosenblum, W.I., Donnenfeld, H., Aleu, F.: Effects of increased blood pressure on cerebral vessels in mice. Arch. Neurol. 14, 631–643 (1966)

    PubMed  CAS  Google Scholar 

  14. Scheffé, H.: Quoted in Snedecor, G.W. and Cochran, W.G.: Statistical Methods. Ames, Iowa: Iowa State University Press 1959, p. 271

    Google Scholar 

  15. Snedecor, G.W., Cochran, W.G.: Statistical Methods. Ames, Iowa: Iowa State University Press 1967, p. 114

    Google Scholar 

  16. Thurel, R.: Cerebromeningeal edema due to arterial hypertension. Abstract: A.M.A. Archives of Neurol. Psychiat. 69, 140 (1953)

    Google Scholar 

  17. Venable, J.H., Coggeshall, R.: A simplified lead citrate stain for use in electron microscopy. J. Cell. Biol, 25, 407–410 (1965)

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Pathology and of Medicine, Queen’s University and Kingston General Hospital, Kingston, Ontario, Canada

    S. Nag, D. M. Robertson, H. B. Dinsdale & R. A. Haas

Authors
  1. S. Nag
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. M. Robertson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. B. Dinsdale
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. A. Haas
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Dept. of Neurology and Neurosurgery, McGill University, 3801 University Street, H3A 2B4, Montreal, Que., Canada

    Hanna M. Pappius Ph. D. (Associate Professor, Associate Neurochemist) & William Feindel M. D., D. Phil., F.R.S.C. (Professor, Director) (Associate Professor, Associate Neurochemist) &  (Professor, Director)

  2. Montreal Neurological Institute, 3801 University Street, H3A 2B4, Montreal, Que., Canada

    Hanna M. Pappius Ph. D. (Associate Professor, Associate Neurochemist) & William Feindel M. D., D. Phil., F.R.S.C. (Professor, Director) (Associate Professor, Associate Neurochemist) &  (Professor, Director)

Rights and permissions

Reprints and permissions

Copyright information

© 1976 Springer-Verlag Berlin · Heidelberg

About this paper

Cite this paper

Nag, S., Robertson, D.M., Dinsdale, H.B., Haas, R.A. (1976). Determination of Cerebral Edema by Quantitative Morphometry. In: Pappius, H.M., Feindel, W. (eds) Dynamics of Brain Edema. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66524-0_7

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-66524-0_7

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-08009-1

  • Online ISBN: 978-3-642-66524-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation