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Formation of the Blastocyst: Determination of Trophoblast and Embryonic Knot

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Developmental Biology and Pathology

Part of the book series: Current Topics in Pathology ((CT PATHOLOGY,volume 62))

Abstract

In mammalian development, the earliest apparent differentiation of cells into two distinct lines manifests itself in the early blastocyst, when trophoblast and embryonic knot (“inner cell mass”) become distinguishable. Recently more and more attention is being paid to this process by developmental biologists, geneticists, cell biologists, reproductive biologists, and oncologists. This is apparently for one of the following reasons: (1) Blastocyst formation seems to result from the first process of determination in mammalian ontogeny, which means that the genome has not been under the influence of any determinative stimuli before. (2) The system appears to be far less complex than later stages so that it may be especially suitable for experimentation. (3) Determination of trophoblast, a tissue of some peculiar properties, may exhibit interesting specific features. (4) Trophoblast is in the focus of interest of reproductive biologists as well as oncologists because of its role in mediating contact between embryo and mother, and because of its invasive growth.

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References

  • Amoroso, E.C., Griffiths, W.F.B., Hamilton, W.J.: The early development of the goat (Capra hirsus). J. Anat. (Lond.) 76, 377–406 (1942).

    CAS  Google Scholar 

  • Assheton, R.: A re-investigation into the early stages of the development of the rabbit. Quart. J. micro Sci. 37, 113–164 (1895).

    Google Scholar 

  • Assheton, R.: The segmentation of the ovum of the sheep, with observations on the hypothesis of a hypoblastic origin for the trophoblast. Quart. J. micro Sci. 41, 205–262 (1898/99).

    Google Scholar 

  • Barlow, P., Owen, D.A.J., Graham, C.: DNA synthesis in the preimplantation mouse embryo. J. Embryol. exp. Morph. 27, 431–445 (1972).

    PubMed  CAS  Google Scholar 

  • Beneden, E. van: Recherches sur l’embryologie des mammifères. La formation des feuillets chez le Lapin. Arch. Biol. 1, 137–224 (1880).

    Google Scholar 

  • Beneden, E. van: Recherches sur les premiers stades du développement du Murin (Vespertilio murinus). Anat. Anz. 16, 305–334 (1899).

    Google Scholar 

  • Beneden, E. van: Recherches sur l’embryologie des mammifères. De la segmentation, de la formation de la cavité blastodermique et de l’embryon didermique chez le Murin. Arch. Biol. 26, 1–63 (1911).

    Google Scholar 

  • Benede, E. van, Julin, C.: Observations sur la maturation, la fécondation et la segmentation de l’oeuf chez les cheiroptères. Arch. Biol. 1, 551–571 (1880).

    Google Scholar 

  • Bernstein, R.M., Mukherjee, B.B.: Control of nuclear RNA synthesis in 2-cell and 4-cell mouse embryos. Nature (Lond.) 238, 457–459 (1972).

    CAS  Google Scholar 

  • Bluntschli, H.: Le développement primaire et l’implantation chez un Centetiné (Hemicentetes). C.R. Ass. Anat. 44, 39–46 (1938).

    Google Scholar 

  • Calarco, P. G., Brown, E.H.: An ultrastructural and cytological study of preimplantation development of the mouse. J. exp. Zool. 171, 253–284 (1969).

    PubMed  CAS  Google Scholar 

  • Chapman, V.M., Whitten, W.K., Ruddle, F.H.: Expression of paternal glucose phosphate isomerase-1 (GPI-1) in preimplantation stages of mouse embryos. Develop. Biol. 26, 153–158 (1971).

    PubMed  CAS  Google Scholar 

  • Church, R.B., Schultz, G.A.: Differential gene activity in the pre-and postimplantation mammalian embryo. Curr. Top. Develop. Biol. 8, 179–202 (1974).

    CAS  Google Scholar 

  • Cole, R.J., Edwards, R.G., Paul, J.: Cytodifferentiation in cell colonies and cell strains derived from cleaving ova and blastocysts of the rabbit. Exp. Cell Res. 37, 501–504 (1965).

    PubMed  CAS  Google Scholar 

  • Cole, R.J., Edwards, R. G., Paul, J.: Cytodifferentiation and embryogenesis in cell colonies and tissue cultures derived from ova and blastocysts of the rabbit. Develop. Biol. 13, 385–407 (1966).

    PubMed  CAS  Google Scholar 

  • Cole, R.J., Paul, J.: Properties of cultured preimplantation mouse and rabbit embryos, and cell strains derived from them. In: Preimplantation Stages of Pregnancy (eds. G.E.W. Wolstenholme, M. O’Connor), pp. 82–122. London: Churchill 1965.

    Google Scholar 

  • Dalcq, A.M.: New descriptive and experimental data concerning the mammalian egg, principally of the rat (With a specification of a previous interpretation). Proc. kon. ned. Akad. Wet., Ser. C, 54, 351–363, 364-372, 469-479 (1951).

    Google Scholar 

  • Dalcq, A.M.: Nouvelles données structurales et cytochimiques sur l’oeuf des mammifères. Rev. gén. Sci. Pures. Appl. 61, 19–41 (1954).

    Google Scholar 

  • Dalcq, A.M.: Processes of synthesis during early development of rodent’s eggs and embryos. In: Studies on Fertility (ed. R.G. Harrison) 7, 113–122 (1955

    Google Scholar 

  • Dalcq, A.M.: Evolution de l’organisation morphogénetique dans l’oocyte chez le Rat et la Souris. Verh. Anat. Ges., Anat. Anz. Suppl. 109, 373–382 (1962a).

    Google Scholar 

  • Dalcq, A.M.: Localisations et evolution des phosphatases aux premiers stades du développement. Bull. Acad. roy. Med. Belg., VIIe Série, 2, 573–610 (1962b).

    CAS  Google Scholar 

  • Dalcq, A.M.: Etudes cyto-enzymologiques sur les~eufs vivants de Souris incubés en présence d’ATP et d’autres mononucléotides. Arch. Biol. 73, 405–444 (1962c).

    CAS  Google Scholar 

  • Dalcq, A.M.: Détection des enzymes de déphosphorylation dans les oeufs de rat et de souris fixés au formol et traités in toto. Arch. Biol. 77, 205–344 (1966).

    Google Scholar 

  • De Geeter, L.: Etudes sur la structure de l’oeuf vierge et les premiers stades du développement chez le Cobaye et le Lapin. Arch. Biol. 65, 363–436 (1954).

    Google Scholar 

  • Denker, H.-W.: Topochemie hochmolekularer Kohlenhydratsubstanzen in Frühentwicklung und Implantation des Kaninchens. II. Beiträge zu entwicklungsphysiologischen Fragestellungen. Zool. Jahrb. Abt. Allg. Zool. Physiol. 75, 246–308 (1970).

    CAS  Google Scholar 

  • Denker, H.-W.: Enzym-Topochemie von Frühentwicklung und Implantation des Kaninchens. III. Proteasen. Histochemie 25,344–360 (1971a).

    CAS  Google Scholar 

  • Denker, H.-W.: In discussion to: J.G. Mulnard: Manipulation of cleaving mammalian embryo... Advanc. Biosci. 6, 255–277 (1971b).

    Google Scholar 

  • Denker, H.-W.: Furchung beim Säugetier: Differenzierung von Trophoblast-und Embryona1-knotenzellen. Verh. Anat. Ges. 66, Anat. Anz. Suppl. 130, 267–272 (1972).

    Google Scholar 

  • Denker, H.-W.: Trophoblastic factors involved in lysis of the blastocyst coverings and in implantation in the rabbit: observations on inversely orientated blastocysts. J. Embryol. exp. Morph. 32, 739–748 (1974).

    PubMed  CAS  Google Scholar 

  • Duval, M.: Etudes sur l’embryologie des Chéiroptères. J. Anat. Physiol. 31, 93–160 (1895).

    Google Scholar 

  • Dvořak, M.: Submicroscopic cytodifferentiation. Ergebn. Anat. Entwickl.-Gesch. 45/4 (1971).

    Google Scholar 

  • Edwards, R.G.: Cleavage of one-and two-celled rabbit eggs in vitro after removal of the zona pellucida. J. Reprod. Fertil. 7, 413–415 (1964).

    PubMed  CAS  Google Scholar 

  • Epstein, C.J.: Expression of the mammalian X chromosome before and after fertilization. Science 175, 1467–1468 (1972).

    PubMed  CAS  Google Scholar 

  • Evans, M.J.: The isolation and properties of a clonal tissue culture strain of pluripotent mouse teratoma cells. J. Embryol. exp. Morph. 28, 163–176 (1972).

    PubMed  CAS  Google Scholar 

  • Gardner, R.L.: Manipulations on the blastocyst. Advanc. Biosci. 6, 279–301 (1971).

    Google Scholar 

  • Gardner, R.L.: An investigation of inner cell mass and trophoblast tissues following their isolation from the mouse blastocyst. J. Embryol. exp. Morph. 28, 279–312 (1972a).

    CAS  Google Scholar 

  • Gardner, R.L.: Manipulation of development. In: Reproduction in Mammals, Book 2: Embryonic and Fetal Development (eds. C.R. Austin, R. V. Short), pp. 110–133. Cambridge University Press 1972b.

    Google Scholar 

  • Gardner, R.L.: Differentiation of trophoblast in the early mammalian embryo. Res. Reprod. 5, No. 5, 1–2 (1973).

    Google Scholar 

  • Gardner, R.L., Johnson, M.H.: Investigation of early mammalian development using interspecific chimaeras between rat and mouse. Nature (New Biol.) 246, 86–89 (1973).

    CAS  Google Scholar 

  • Glass, R.H., Calarco, P.G., Lin, T.P., Florence, J., Oh, J.O.: Development of the mouse blastocyst following injection with Newcastle disease virus. Biol. Reprod. 10, 502–511 (1974).

    PubMed  CAS  Google Scholar 

  • Goetz, R.H.: Studien zur Placentation der Centetiden. II. Die Implantation und Frühentwicklung von Hemicentetes semispinosus (Cuvier). Z. Anat. Entwickl.Gesch. 107, 274–318 (1937).

    Google Scholar 

  • Goetz, R.H.: On the early development of the Tenrecoidea (Hemicentetes semispinosus). Bio-Morphosis 1, 67–79 (1939).

    Google Scholar 

  • Golbus, M.S., Calarco, P.G., Epstein, C.J.: The effects of inhibitors of RNA synthesis (α-amanitin and actinomycin D) on preimplantation mouse embryogenesis. J. exp. Zool. 186, 207–216 (1973).

    PubMed  CAS  Google Scholar 

  • Graham, C.F.: The design of the mouse blastocyst. Symp. Soc. exp. Biol. 25, 371–378 (1971).

    PubMed  CAS  Google Scholar 

  • Graham, C.F.: Nucleic acid metabolism during early mammalian development. In: The Regulation of Mammalian Reproduction (eds. S.J. Segal et al.), pp. 286–301. Springfield, Ill.: Charles C.Thomas 1973.

    Google Scholar 

  • Gurdon, J.B.: The developmental capacity of nuclei taken from intestinal epithelium cells of feeding tadpoles. J. Embryol. exp. Morph. 10, 622–640 (1962).

    PubMed  CAS  Google Scholar 

  • Gurdon, J.B., Woodland, H.R.: The influence of the cytoplasm on the nucleus during cell differentiation, with special reference to RNA synthesis during amphibian cleavage. Proc. roy. Soc. B 173, 99–111 (1969).

    CAS  Google Scholar 

  • Hartman, C. G.: Studies on the development of the opossum Didelphys virginiana L. III. Description of new material on maturation, cleavage and entoderm formation. IV. The bilaminar blastocyst. J. Morph. 32, 1–142 (1919).

    Google Scholar 

  • Heape, W.: The development of the mole (Talpa europea), the ovarian ovum, and segmentation of the ovum. Quart. J. micr. Sci. 26, 157–174 (1886).

    Google Scholar 

  • Herbert, M.C., Graham, C.F.: Cell determination and biochemical differentiation of the early mammalian embryo. Curr. Top. Develop. Biol. 8, 151–178 (1974).

    CAS  Google Scholar 

  • Hesseldahl, H.: Ulstrastructure of early cleavage stages and preimplantation in the rabbit. Z. Anat. Entwickl. Gesch. 135, 139–155 (1971).

    CAS  Google Scholar 

  • Heuser, C.H., Streeter, G.L.: Early stages in the development of pig embryos, from the period of initial cleavage to the time of the appearance of limb-buds. Contr. Embryol. Carneg. Inst. 20, 1–29 (1929).

    Google Scholar 

  • Hill, J.P.: The early development of the marsupialia, with special reference to the native cat (Dasyurus viverrinus). (Contributions to the embryology of the marsupialia, IV.). Quart. J. micro Sci. 56, 1–134 (1911).

    Google Scholar 

  • Hillman, N., Hillman, R., Wileman, G.: Ultrastructural studies of cleavage stage t1 2/t1 2 mouse embryos. Amer. J. Anat. 128, 311–340 (1970).

    PubMed  CAS  Google Scholar 

  • Hillman, N., Sherman, M.I, Graham, C.: The effect of spacial arrangement on cell determination during mouse development. J. Embryol. exp. Morph. 28, 263–278 (1972).

    PubMed  CAS  Google Scholar 

  • Ishida, K.: Enzymohistochemical studies of differentiated 8–16 cell eggs of the hamster. Jap. J. Animal Reprod. 18, 105–109 (1972).

    Google Scholar 

  • Jones-Seaton, A.: Etude de l’organisation cytoplasmique de l’oeuf des rongeurs, principalement quant à la basophilie ribonucléique. Arch. Biol. 61, 291–444 (1950).

    CAS  Google Scholar 

  • Krauskopf, C.: Elektronenmikroskopische Untersuchungen über die Struktur der Oozyte und des 2-Zellenstadiums beim Kaninchen. II. Blastomeren. Z. Zellforsch. 92, 296–312 (1968).

    PubMed  CAS  Google Scholar 

  • Manes, C.: The participation of the embryonic genome during early cleavage in the rabbit. Develop. Biol. 32, 453–459 (1973).

    PubMed  CAS  Google Scholar 

  • Mintz, B.: Formation of genotypically mosaic mouse embryos. Amer. Zool. 2, 432 (1962).

    Google Scholar 

  • Mintz, B.: Gene expression in the morula stage of mouse embryos, as observed during development of t1 2 /t1 2 lethal mutants in vitro. J. exp. Zool. 157, 267–271 (1964a).

    CAS  Google Scholar 

  • Mintz, B.: Synthetic processes and early development in the mammalian egg. J. exp. Zool. 157, 85–100 (1964b).

    CAS  Google Scholar 

  • Mintz, B.: Experimental genetic mosaicism in the mouse. In: Preimplantation Stages of Pregnancy (eds. G.E.W. Wolstenholme, M. O’Connor), pp. 194–216. London: Churchill 196

    Google Scholar 

  • Mintz, B.: Clonal basis of mammalian differentiation. Symp. Soc. exp. Biol. 25, 345–370 (1971).

    PubMed  CAS  Google Scholar 

  • Moore, N. W., Adams, C.E., Rowson, L.E.A.: Developmental potential of single blastomeres of the rabbit egg. J. Reprod. Fertil. 17, 527–531 (1968).

    PubMed  CAS  Google Scholar 

  • Moskalewski, S., Koprowski, H.: Presence of egg antigen in immature oocytes and preimplantation embryos. Nature (Lond.) 237, 167–168 (1972).

    CAS  Google Scholar 

  • Mulnard, J.: Contribution à la connaissance des enzymes dans l’ontogénèse. Les phosphomonoestérases acide et alkaline dans le développement du Rat et de la Souris. Arch. Biol. 66, 525–685 (1955).

    CAS  Google Scholar 

  • Mulnard, J.: Aspects cytochimiques de la régulation in vitro de l’oeuf de souris après déstruction d’un des blastomeres du stade II. I. La phosphomonoestérase acide. Bull. Acad. roy. Med. Belg., Series 2, 5, 31–67 (1965).

    CAS  Google Scholar 

  • Mulnard, J.: Les mécanismes de la regulation aux premiers stades du développement des mammifères. Bull. Soc. zool. Fr. 91, 253–277 (1966).

    Google Scholar 

  • Mulnard, J.: Analyse microcinematographique du développment de l’oeuf de souris du stade II au blastocyste. Arch. Biol. 78, 107–138 (1967).

    CAS  Google Scholar 

  • Mulnard, J., Dalcq, A.M.: Les polysaccharides dans le développement de l’oeuf tubaire du Rat. C.R. Soc. Biol. (Paris) 149,836–839 (1955).

    CAS  Google Scholar 

  • Rodé, B., Damjanov, I., Škreb, N.: Distribution of acid and alkaline phosphatase activity in early stages of rat embryos. Bull. Sci., Cons. Acad. RSF Yougoslavie, Sect. A, 13, 304 (1968).

    Google Scholar 

  • Schlafke, S., Enders, A.C.: Cytological changes during cleavage and blastocyst formation in the rat. J. Anat. (Lond.) 102, 13–32 (1967).

    CAS  Google Scholar 

  • Seidel, F.: Entwicklungsphysiologie des Insektenkeimes. Verh. dtsch. zool. Ges., Freiburg p. 291–336 (1936).

    Google Scholar 

  • Seidel, F.: Die Entwicklungspotenzen einer isolierten Blastomere des Zweizellenstadiums im Säugetierei. Naturwissenschaften 39, 355–356 (1952).

    Google Scholar 

  • Seidel, F.: Entwicklungsphysiologie der Tiere. I. Ei und Furchung. Sammlung Göschen Band 1162. Berlin: Walter de Gruyter & Co. 1953.

    Google Scholar 

  • Seidel, F.: Nachweis eines Zentrums zur Bildung der Keimscheibe im Säugetierei. Naturwissenschaften 43, 306–307 (1956).

    Google Scholar 

  • Seidel, F.: Die Entwicklungsfähigkeiten isolierter Furchungszellen aus dem Ei des Kaninchens Oryctolagus cuniculus. Wilhelm Roux’ Arch. Entwicklungsmech. Organismen 152, 43–130 (1960).

    Google Scholar 

  • Seidel, F.: Entwicklungspotenzen des friihen Säugetierkeimes. Arbeitsgemeinschaft für Forschung des Landes Nordrhein-Westfalen, Heft 193, pp. 1–91. Köln-Opladen: Westdeutscher Verlag 1969.

    Google Scholar 

  • Sherman, M.I.: Biochemistry of differentiation of mouse trophoblast: esterase. Exp. Cell Res. 75, 449–459 (1972).

    PubMed  CAS  Google Scholar 

  • Skalko, R.G.: Methods for histologic and autoradiographic analysis of the early mouse embryo. In: Methods in Mammalian Embryology (ed. I.C. Daniel, jr.). pp. 238–246. San Francisco: Freeman 1971.

    Google Scholar 

  • Spemann, H.: Experimentelle Beiträge zu einer Theorie der Entwicklung. Berlin: Springer 1936. Reprint Berlin-Heidelberg-New York: Springer 1968.

    Google Scholar 

  • Stern, M.S.: Experimental studies on the organization of the preimplantation mouse embryo. II. Reaggregation of disaggregated embryos. J. Embryol. exp. Morph. 28, 255–261 (1972).

    PubMed  CAS  Google Scholar 

  • Stern, M.S.: Chimaeras obtained by aggregation of mouse eggs with rat eggs. Nature (Lond.) 243, 472–473 (1973).

    CAS  Google Scholar 

  • Stern, M.S., Wilson, I.B.: Experimental studies on the organization of the preimplantation mouse embryo. I. Fusion of asynchronously cleaving eggs. J. Embryol. exp. Morph. 28, 247–254 (1972).

    PubMed  CAS  Google Scholar 

  • Tarkowski, A.K.: Experiments on the development of isolated blastomeres of mouse eggs. Nature (Lond.) 184, 1286–1287 (1959).

    CAS  Google Scholar 

  • Tarkowski, A.K.: Mouse chimaeras developed from fused eggs. Nature (Lond.) 190, 857–860 (1961).

    CAS  Google Scholar 

  • Tarkowski, A.K.: Embryonic and postnatal development of mouse chimeras. In: Preimplantation Stages of Pregnancy (eds. G.E. W. Wolstenholme, M. O’Connor), pp. 183–193. London: Churchill 1965.

    Google Scholar 

  • Tarkowski, A.K., Wróblewska, J.: Development of blastomeres of mouse eggs isolated at the 4-and 8-cell stage. J. Embryol. exp. Morph. 18, 155–180 (1967).

    PubMed  CAS  Google Scholar 

  • Wilson, I.B., Bolton, E., Cuttler, R.H.: Preimplantation differentiation in the mouse egg as revealed by microinjection of vital markers. J. Embryol. exp. Morph. 27, 467–479 (1972).

    PubMed  CAS  Google Scholar 

  • Wimsatt, W.A.: An analysis of implantation in the bat, Myotis lucifugus lucifugus. Amer. J. Anat. 74, 355–411 (1944).

    Google Scholar 

  • Wimsatt, W.A.: Some comparative aspects of implantation. Biol. Reprod. 12, 1–40 (1975).

    PubMed  CAS  Google Scholar 

  • Woodland, H.R., Graham, C.F.: RNA synthesis during early development of the mouse. Nature (Lond.) 221, 327–332 (1969).

    CAS  Google Scholar 

  • Zeilmaker, G.H.: Fusion of rat and mouse morulae and formation of chimaeric b1astocysts. Nature (Lond.) 242, 115–116 (1973).

    CAS  Google Scholar 

Note added in Proof

  • Avendano, S., Croxatto, H.D., Pereda, J., Croxatto, H.B.: A seven-cell human egg recovered from the oviduct. Fertil. Steril. 26, 1167–1172 (1975).

    PubMed  CAS  Google Scholar 

  • Damjanov, I., Solter, D.: Experimental teratoma. Current Topics in Pathology (eds. E. Grundmann, W.H. Kirsten). 59, 69–130 (1974).

    Google Scholar 

  • Ducibella, Th., Albertini, D.F., Anderson, E., Biggers, J.D.: The preimplantation mammalian embryo. Characterization of intercellular junctions and their appearance during development. Devel. Biol. 45, 231–250 (1975).

    CAS  Google Scholar 

  • Dudbella, Th., Anderson, E.: Cell shape and membrane changes in the eight-cell mouse embryo. Prerequisites for morphogenesis of the blastocyst. Develop. Biol. 47, 45–58 (1975).

    Google Scholar 

  • Ford, C.E., Evans, E.P., Gardner, R.L.: Marker chromosome analysis of two mouse chimaeras. J. Embryol. exp. Morph. 33, 447–457 (1975).

    PubMed  CAS  Google Scholar 

  • Garner, W., McLaren, A.: Cell distribution in chimaeric mouse embryos before implantation. J. Embryol. exp. Morph. 32, 495–503 (1974).

    PubMed  CAS  Google Scholar 

  • Gulyas, B.J.: A reexamination of cleavage patterns in eutherian mammalian eggs. Rotation of blastomere pairs during second cleavage in the rabbit. J. exp. Zool. 193, 235–248 (1975).

    PubMed  CAS  Google Scholar 

  • Izquierdo, L., Marticorena, P.: Alkaline phosphatase in preimplantation mouse embryos. Exp. Cell Res. 92, 399–402 (1975).

    PubMed  CAS  Google Scholar 

  • Izquierdo, L., Ortiz, M.E.: Differentiation in the mouse morulae. Wilh. Roux’ Arch. 177, 67–74 (1975).

    Google Scholar 

  • Panigel, M., Kraemer, D.C., Kalter, S.S., Smith, G.S., Heberling, R.L.: Ultrastructure of cleavage stages and preimplantation embryos of the baboon. Anat. Embryol. 147, 45–62 (1975).

    PubMed  CAS  Google Scholar 

  • Rossant, J.: Investigation of the determinative state of the mouse inner cell mass. I. Aggregation of isolated inner cell masses with morulae. J. Embryol. Exp. Morph. 33, 979–990 (1975).

    PubMed  CAS  Google Scholar 

  • Rossant, J.: Invesitgation of the determinative state of the mouse inner cell mass. II. The fate of isolated inner cell masses transferred to the oviduct. J. Embryol. exp. Morph. 33, 991–1001 (1975).

    PubMed  CAS  Google Scholar 

  • Sherman, M.I.: The role of cell-cell interaction during early mouse embryogenesis. In: The Early Development of Mammals (British Society for Developmental Biology Symposium 2), pp. 145–164. Cambridge University Press, Cambridge 1975.

    Google Scholar 

  • Sherman, M.I.: Long term culture of cells derived from mouse blastocyts. Differentiation 3, 51–68 (1975).

    PubMed  CAS  Google Scholar 

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Denker, HW. (1976). Formation of the Blastocyst: Determination of Trophoblast and Embryonic Knot. In: Gropp, A., Benirschke, K. (eds) Developmental Biology and Pathology. Current Topics in Pathology, vol 62. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66458-8_5

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