Abstract
Extensive studies in recent years have established that DNA replication is a complex reaction, requiring a variety of enzymes and protein factors that are involved in the initiation and elongation of deoxynucleotide chains. The identification of the components of the DNA synthesizing apparatus as well as the clarification of their specific functions has now become of vital interest in biochemistry. Progress in this area of research can be expected from the use of conditionally lethal mutants exhibiting temperature sensitive DNA synthesis. Provided the availability of adequate test methods, such mutants prove most valuable for the isolation and characterization of constituents of the replication machinery. This was recently demonstrated by the isolation of several proteins involved in the replication of bacterial and phage DNA (Kornberg, 1974).
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References
Benz, W.C., Strominger, J.L.: Viral and cellular DNA synthesis in nuclei from human lymphocytes transformed by Epstein-Barr virus. Proc. Nat. Acad. Sci. USA 72, 2413–2417 (1975)
Golombek, J., Wolf, W., Wintersberger, E.: DNA synthesis activity in synchronized yeast cells. Molec. Gen. Genet. 132, 137–145 (1974)
Hartwell, L.H., Culotti, J., Pringle, J.R., Reid, B.J.: Genetic control of the cell division cycle in yeast. Science 183, 46–51 (1974)
Hershy, V.W., Stieber, J.F., Mueller, G.C.: DNA synthesis in isolated HeLa nuclei. A system for continuation of replication in vivo. Eur. J. Biochem. 34, 383–394 (1973)
Kornberg, A.: DNA Synthesis. San Francisco: W.H. Freeman and Company, 1974
Loeb, L.: Eukaryotic DNA polymerases. In: The Enzymes. New York: Academic Press, 1974, Vol. 10, pp. 173–209
Newlon, C.S., Petes, T.D., Hereford, L.M., Fangman, W.L.: Replication of yeast chromosomal DNA. Nature (Lond.) 247, 32–35 (1973)
Schiebel, W., Schneck, U.: DNA replication continued in isolated nuclei of synchronously growing Physarum polycephalum. Hoppe-Seyler’s Z. Physiol. Chem. 355, 1515–1525 (1974)
Winnacker, E.L., Magnusson, G., Reichard, P.: Synthesis of polyoma DNA by isolated nuclei. Biochem. Biophys. Res. Comm. 44, 952–957 (1971)
Wintersberger, E.: DNA polymerases from yeast. Further purification and characterization of DNA-dependent DNA polymerases A and B. Eur. J. Biochem. 50, 41–47 (1974)
Wintersberger, U.: Absence of a low molecular weight DNA-polymerase from nuclei of the yeast, Saccharomyces cerevisiae. Eur. J. Biochem. 50, 197–202 (1974)
Wintersberger, U., Binder, M., Fischer, P.: Cytogenetic demonstration of mitotic chromosomes in the yeast Saccharomyces cerevisiae. Molec. Gen. Genet. (1975, in press)
Wintersberger, U., Hirsch, J., Fink, A.M.: Studies on nuclear and mitochondrial DNA-replication.in a temperature-sensitive mutant of Saccharomyces cerevisiae. Molec. Gen. Genet. 131, 291–299 (1974)
Wintersberger, U., Smith, P., Letnansky, K.: Yeast chromatin. Preparation from isolated nuclei, histone composition and transcription capacity. Eur. J. Biochem. 33, 123–130 (1973)
Wintersberger, U., Wintersberger, E.: Studies on DNA polymerases from yeast. I. Partial purification and properties of two DNA-polymerases from mitochondria-free cell extracts. Eur. J. Biochem. 13, 11–19 (1970a)
Wintersberger, U., Wintersberger, E.: Studies on DNA polymerases from yeast. II. Partial purification and characterization of mitochondrial DNA polymerase from wild type and respiration deficient yeast cells. Eur. J. Biochem. 13, 20–27 (1970b)
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Wintersberger, U. (1976). Studies on DNA Synthesis in Saccharomyces cerevisiae . In: Kiefer, J. (eds) Radiation and Cellular Control Processes. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66455-7_6
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DOI: https://doi.org/10.1007/978-3-642-66455-7_6
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