Advertisement

Macromolecular Events Following Nutritional Shift-Up of Tetrahymena

  • R. E. Pearlman
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Tetrahymena pyriformis is a unicellular eukaryotic organism that has been much used in biochemical and physiologic studies of the cell cycle and the control of cell growth and division (Hill, 1972; Elliott, 1973). Tetrahymena has a short generation time (2 1/2 h under most conditions), can be readily grown in large amounts, is readily labelled by nucleic acid and protein precursors, and both enzymes and nucleic acids from these cells are readily isolated and handled. Also, recent technical advances have made mutant isolation and genetic analysis with this organism very feasible (Gardonio et al., 1975; Orias and Bruns, 1975).

Keywords

Excision Repair Recent Technical Advance Preferential Replication Irradiate Culture Inbred Family 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Brunk, C.F.: The time sequence of events during excision repair in Tetrahymena pyriformis. Photochem. Photobiol. 15, 349–360 (1972)PubMedCrossRefGoogle Scholar
  2. Bruns, P., Brussard, T.: Positive selection for mating with functional heterokaryons in Tetrahymena pyriformis. Genetics 78, 831–841 (1974)PubMedGoogle Scholar
  3. Cameron, I.L., Bols, N.C.: Effect of cell population density on G2 arrest in Tetrahymena. J. Cell. Biol. 67, 518–522 (1975)PubMedCrossRefGoogle Scholar
  4. Cameron, I.L., Griffin, E.E., Rudick, M.J.: Macromolecular events following refeeding of starved Tetrahymena. Exptl. Cell Res. 65, 265–272 (1971)PubMedCrossRefGoogle Scholar
  5. Cameron, I.L., Jeter, J.R.: Synchronization of the cell cycle of Tetrahymena by starvation and refeeding. J. Protozool. 17, 429–431 (1970)PubMedGoogle Scholar
  6. Elliott, A.M.: Biology of Tetrahymena. Dowden, Hutchinson and Ross, Stroudsburg, Pennsylvania 1973Google Scholar
  7. Engberg, J., Christiansen, G., Leick, V.: Autonomous rDNA molecules containing single copies of the ribosomal RNA genes in the macronucleus of Tetrahymena pyriformis. Biochem. Biophys. Res. Comm. 59, 1356–1365 (1974a).PubMedCrossRefGoogle Scholar
  8. Engberg, J., Collins, J., Andersson, P., Leick, V.: The free rDNA molecules from Tetrahymena pyriformis GL are giant polindromes. J. Mol. Biol. (1976)Google Scholar
  9. Engberg, J., Mowat, D., Pearlman, R.E.: Preferential replication of the ribosomal RNA genes during a nutritional shift-up in Tetrahymena pyriformis. Biochim. Biophys. Acta 272, 312–320 (1972)Google Scholar
  10. Engberg, J., Nilsson, J.R., Pearlman, R.E., Leick, V.: Induction of nucleolar and mitochondrial DNA replication in Tetrahymena pyriformis. Proc. Nat. Acad. Sci. U.S.A. 71, 894–898 (1974b)CrossRefGoogle Scholar
  11. Engberg, J., Pearlman, R.E.: The amount of ribosomal RNA genes in Tetrahymena pyriformis in different physiological states. Eur. J. Biochem. 26, 393–400 (1972)PubMedCrossRefGoogle Scholar
  12. Gall, J.G.: Free ribosomal RNA genes in the macronucleus of Tetrahymena. Proc. Nat. Acad. Sci. U.S.A. 71, 3078–3081 (1974)CrossRefGoogle Scholar
  13. Gardonio, E., Crerar, M., Pearlman, R.E.: Manipulations with Tetrahymena pyriformis on solid medium. Methods in Cell Biology (ed. D.M. Prescott) Academic Press Vol. IX, 329–348 (1975)Google Scholar
  14. Hellung-Larsen, P.: Liquid scintillation counting of 3H and 32P RNA in slices of Polyacrylamide gels. Anal. Biochem. 39, 454–461 (1971)PubMedCrossRefGoogle Scholar
  15. Hill, D.L.: Biochemistry and Physiology of Tetrahymena. New York: Academic Press 1972Google Scholar
  16. Jeter, J.R., Pavlat, W.A., Cameron, I.L.: Changes in the nuclear acidic proteins and chromatin structure in starved and refed Tetrahymena. Exptl. Cell Res. 93, 79–88 (1975)PubMedCrossRefGoogle Scholar
  17. Karrer, K.M., Gall, J.G.: The macronuclear ribosomal DNA of Tetrahymena pyriformis is a palindrome. J. Mol. Biol (1976)Google Scholar
  18. Keiding, J., Westergaard, O.: Induction of DNA polymerase activity in irradiated Tetrahymena cells. Exptl. Cell Res. 64, 317–322 (1971)PubMedCrossRefGoogle Scholar
  19. Leick, V., Plesner, P.: Formation of ribosomes in Tetrahymena pyriformis. Biochim. Biophys. Acta 169, 398–408 (1968)PubMedGoogle Scholar
  20. Leick, V., Tønnesen, T., Engberg, J., Nilsson, J.R.: Nucleolar reorganization in Tetrahymena pyriformis GL during a nutritional shift-up; timing of ribosomal RNA transcription and ribosomal DNA replication. Proceedings of the 9th Conference of the Nordic Society for Cell Biology, Odense University Press, Denmark 1975Google Scholar
  21. Mowat, D., Pearlman, R.E.: Excision repair in Tetrahymena: Events following refeeding of starved UV-irradiated cells. Photochem. Photobiol. 21, 5–11 (1975)PubMedCrossRefGoogle Scholar
  22. Mowat, D., Pearlman, R.E., Engberg, J.: DNA synthesis following refeeding of starved Tetrahymena pyriformis GL. Exptl. Cell Res. 84, 282–286 (1974)PubMedCrossRefGoogle Scholar
  23. Nilsson, J.R., Leick, V.: Nucleolar organization and ribosome formation in Tetrahymena pyriformis GL. Exptl. Cell Res. 60, 361–372 (1970)PubMedCrossRefGoogle Scholar
  24. Orias, E., Bruns, P.: Induction and isolation of mutants in Tetrahymena. Methods in Cell Biology (ed. D.M. Prescott). New York: Academic Press 1975Google Scholar
  25. Rasmussen, L., Modeweg-Hansen, L.: Cell multiplication in Tetrahymena cultures after addition of particulate material. J. Cell Sci. 12, 275–286 (1973)PubMedGoogle Scholar
  26. Rudick, M.J., Cameron I.L.: Regulation of DNA synthesis and cell division in starved — refed synchronized Tetrahymena pyriformis HSM. Exptl. Cell Res. 70, 411–416 (1972)PubMedCrossRefGoogle Scholar
  27. Salamone, M., Pearlman, R.E.: Abstract to annual meeting of American Society for Cell Biology Puerto Rico 1975Google Scholar
  28. Stocco, D.M., Zimmerman, A.M.: Metabolism of acid — soluble nucleotides in starved - refed synchronized Tetrahymena pyriformis GL. Can. J. Biochem. 52, 310–318 (1974)PubMedCrossRefGoogle Scholar
  29. Westergaard, O., Pearlman, R.E.: DNA polymerase activity in methotrexate plus uridine treated Tetrahymena. Exptl. Cell Res. 54, 309–313 (1969)PubMedCrossRefGoogle Scholar
  30. Wolfe, J.: Conjugation in Tetrahymena: The relationship between the division cycle and cell pairing. Dev. Biol. 35, 221–231 (1973)PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1976

Authors and Affiliations

  • R. E. Pearlman

There are no affiliations available

Personalised recommendations