Abstract
It is known that the cells of the yeast Sacchavomyces cevevisiae exhibit a postirradiation increase in colony-forming ability after delayed plating. This phenomenon has been termed liquid holding recovery (LHR) (Patrick and Haynes, 1964). LHR is dependent upon the availability of metabolic energy (Jain and Pohlit, 1972). Respiratory deficient yeast strains must be supplemented with glucose in the LHR buffer to perform LHR.
This work is part of the Ph.D. thesis of the first author.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Bernardi, G., Carnevali, F., Nicolaieff, A., Piperno, G., Tecce, G.: Separation and characterization of a satellite DNA from a yeast cytoplasmic “petite” mutant. J. Molec. Biol. 37, 493–505 (1968)
Brendel, M., Fäth, W.W.: Isolation and characterization of mutants of Saccharomyces cevevisiae auxotrophic and conditionally auxotrophic for 5′-dTMP. Z. Naturforsch. 29c, 733–738 (1974)
Byers, B., Goetsch, L.: Duplication of spindle plaques and integration of the yeast cell cycle. Cold Spring Harbor Symp. Quant. Biol. 28, 123–131 (1973)
Eaton, N.R.: New press for disruption of microorganism. J. Bacteriol. 83, 7359–7360 (1962)
Frey, R., Hagen, U.: Oxygen effect on y-irradiated NDA. Radiat. Environ. Biophys. 11, 125–133 (1974)
Jain, V.K., Pohlit, W.: Influence of energy metabolism on the repair of x-ray damage in living cells. Biophysik 8, 254–263 (1972)
Moens, P.B., Rapport, E.: Spindles, spindle plaques and meiosis in the yeast Saccharomyces cerevisiae (Hansen). J. Cell Biol. 50, 344–361 (1971)
Painter, R.B., Young, B.R.: Repair replication in mammalian cells after x-irradiation. Mutation Res. 14, 225–235 (1972)
Patrick, M.H., Haynes, R.H.: Dark recovery phenomena in yeast. II. Conditions that modify the recovery process. Radiat. Res. 23, 564–579 (1964)
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1976 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Höltz, G.W., Brendel, M. (1976). Incorporation of (Methyl 3H) 5′-dTMP into DNA of Diploid Yeast Cells as Tracer of Liquid Holding Recovery after X-Ray Irradiation. In: Kiefer, J. (eds) Radiation and Cellular Control Processes. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66455-7_15
Download citation
DOI: https://doi.org/10.1007/978-3-642-66455-7_15
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-66457-1
Online ISBN: 978-3-642-66455-7
eBook Packages: Springer Book Archive