Abstract
Mechanisms of the lipolytic activity of essential phospholipids (EPL) were studied by incubating samples of epididymal adipose tissue from WISTAR rats with EPL or/and norepinephrine and/or theophylline in a Krebs-solution (pH 7.2, T = 37°C). The levels of free fatty acids and of glycerol were measured in the tissue and in the incubation medium, the ATP content of the tissue was determined and the cAMP was isolated.
EPL (10−6 − 10−3M) did not influence the basic spontaneous lipolytic activity in adipose tissue, but inhibited — dependent on dose — the increase of lipolytic activity induced by norepinephrine (10−6 − 10−4M), or theophylline (3x10−3M) or dibutyryl cAMP (10−3M). EPL reduced the decrease of ATP content in adipose tissue caused by norepinephrine or theophylline. Further, EPL inhibited the increase of cAMP synthesis elicited by norepinephrine or norepinephrine + theophylline.
The lipolytic activity of EPL was studied also in vivo by administering EPL (750 mg/kg, i.p.) to rats 120 or 240 min before sacrificing them. Further, the effect on the stimulation of lipolysis was investigated by additionally injecting 0.5 mg/kg norepinephrine i.p. 30 min before sacrificing.
EPL markedly increased the lipolytic activity in vivo as measure by the levels of free fatty acids and of glycerol in the blood serum. Also an inhibition of the increased lipolysis due to the action of norepinephrine was observed.
From the discrepancy in the effects of EPL in vitro and in vivo it is hypothesized that EPL activates the lipoprotein lipase and the hepatic lipase, but inhibits the hormone-sensitive lipase in adipose tissue.
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Szyszka, K. (1976). Influence of EPL on Lipolysis in Vitro and in Vivo. In: Peeters, H. (eds) Phosphatidylcholine. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66424-3_9
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DOI: https://doi.org/10.1007/978-3-642-66424-3_9
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