Skip to main content

Abstract

The six steps of preparation which we consider essential are as follows:

  1. 1.

    Washing of the samples

  2. 2.

    Pinning

  3. 3.

    Fixation

  4. 4.

    Dehydration

  5. 5.

    Critical point drying

  6. 6.

    Sputter coating

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

eBook
USD 16.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Anderson, T.P.: Techniques for the preservation of three dimensional structure in preparing specimens for the electron microscope. Trans. N.Y. Acad. Sci. 13, 130–133 (1951).

    Google Scholar 

  • DeNee, P.B., Walker, E.R.: Specimen coating technique for the SEM—a comparative study. In: Scanning Electron Microscopy (eds. O. Johari and I. Corvin ). Chicago: IITRI, 1975, pp. 225–232.

    Google Scholar 

  • Echlin, P.: Sputter coating techniques for scanning electron microscopy. In: Scanning Electron Microscopy (eds. O. Johari and I. Corvin ). Chicago: IITRI, 1975, pp. 217–224.

    Google Scholar 

  • Ferenczy, A., Richart, R.M.: Female Reproductive System: Dynamics of Scan and Transmission Electron Microscopy. New York-London-Sydney-Toronto: John Wiley & Sons, 1974.

    Google Scholar 

  • Fromme, H.G., Pfautsch, M., Pfefferkorn, G., Bystricky, V.: Die „Kritische Punkt”-Trocknung als Präparationsmethode für die Rasterelektronenmikroskopie. Micros. Acta 73, 29–37 (1972).

    CAS  Google Scholar 

  • Hafez, E.S.E.: Scanning Electron Microscopic Atlas of Mammalian Reproduction. Tokyo: Igaku Shoin, 1975

    Google Scholar 

  • Kessel, R.G., Shih, C.Y.: Scanning Electron Microscopy in Biology. A Student’s Atlas on Biological Organization. Berlin-Heidelberg-New York: Springer, 1974.

    Google Scholar 

  • Lampert, F., Koschorek, F.: Elektronenmikroskopische Präparation biologischer Objekte ohne Dünnschnittechnik durch Oberflächenspreitung und Kritische-Punkt Trocknung. Z. Kinderheilk. 111, 29–37 (1971).

    Article  PubMed  CAS  Google Scholar 

  • Ludwig, H., Patek, E., Nilsson, L., Metzger, H., Hafez, E.S.E.: Methodology of scanning electron microscopy. In: SEM Atlas of Mammalian Reproduction (ed. E.S.E. Hafez ). Tokyo: Igaku Shoin, 1975, pp. 1–17.

    Google Scholar 

  • Ohnsorge, J., Holm, R.: Rasterelektronenmikroskopie. Eine Einführung für Mediziner und Biologen. Stuttgart: Thieme, 1973.

    Google Scholar 

  • Reimer, L., Pfefferkorn, G.: Rasterelektronenmikroskopie. Berlin-Heidelberg-New York: Springer, 1973.

    Google Scholar 

  • Revel, J.-P.: Elements of scanning electron microscopy for biologists. In: Scanning electron microscopy (eds. O. Johari and I. Corvin ). Chicago: IITRI, 1975, pp. 687–696.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

Copyright information

© 1976 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Ludwig, H., Metzger, H. (1976). Methodology. In: The Human Female Reproductive Tract. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66345-1_3

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-66345-1_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-66347-5

  • Online ISBN: 978-3-642-66345-1

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics