Abstract

Since the discovery of the double helix by Watson and Crick, and the intensive studies of the Escherichia coli DNA polymerase I by Kornberg and coworkers, the understanding of DNA replication has been controversial in many aspects. Some basic ideas have been generally accepted: (1) the DNA is a helical double strand with antiparallel chains; (2) the immediate precursors for DNA synthesis are 5′-deoxynucleoside triphosphates; (3) the replication is semiconservative; and (4) all DNA is synthesized exclusively in 5′→3′ direction. The synthesis of larger DNA appears to be discontinuous in one or often both strands, and no DNA polymerase can start synthesis on a template without a primer.

Keywords

Hydrolysis Recombination Toluene Lipase Caffeine 

Abbreviations

ColE1

colicinogenic factor E1 (plasmid coding for colicin E1)

EcoRI

restriction enzyme from E. coli bearing resistance transfer factor I (plasmid)

FV

F-pili specific, filamentous virus (bacteriophages fd, f1, M13)

HpaII

restriction enzyme from Haemophilus parainfluenzae

NAD

nicotinamide adenine dinucleotide

NEM

N-ethyl maleimide

NMN

nicotinamide mononucleotide

pol

polymerase

RF

replicative form DNA of small phages (II: a discontinuity in one strand; I: doubly closed)

SDS

sodium dodecyl sulfate

SS

single strand

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© Springer-Verlag, Berlin · Heidelberg 1976

Authors and Affiliations

  • Klaus Geider
    • 1
  1. 1.Abteilung Molekulare BiologieMax-Planck-Institut für Medizinische ForschungHeidelbergFederal Republic of Germany

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