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Abstract

Porcine thyroid cells, isolated by trypsinization from adult glands and cultured in vitro in a medium containing thyrotropin (TSH), begin to reassociate within 15 min and, within a week, or more when hydrocortisone is added to TSH, regain their previous in vivo follicular organization. Cells cultured without TSH-stimulation behave similarly, though transiently during only the first days of culture. After about four days a monolayer culture extend over the surface of the plastic support. The TSH-stimulated cells also lose their three-dimensional organization after more than a week.

Keywords

Thyroid Cell Thyroid Follicle Cellular Slime Mold Plastic Support Follicular Lumen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer-Verlag Berlin-Heidelberg 1976

Authors and Affiliations

  • Pierre Cau
    • 1
  • Marc Michel-Béchet
    • 1
  • Guy Fayet
    • 1
  1. 1.Laboratoire d’Histologie I, Faculté de MédecineMarseille Cedex 4France

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