Porcine thyroid cells, isolated by trypsinization from adult glands and cultured in vitro in a medium containing thyrotropin (TSH), begin to reassociate within 15 min and, within a week, or more when hydrocortisone is added to TSH, regain their previous in vivo follicular organization. Cells cultured without TSH-stimulation behave similarly, though transiently during only the first days of culture. After about four days a monolayer culture extend over the surface of the plastic support. The TSH-stimulated cells also lose their three-dimensional organization after more than a week.


Thyroid Cell Thyroid Follicle Cellular Slime Mold Plastic Support Follicular Lumen 
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Copyright information

© Springer-Verlag Berlin-Heidelberg 1976

Authors and Affiliations

  • Pierre Cau
    • 1
  • Marc Michel-Béchet
    • 1
  • Guy Fayet
    • 1
  1. 1.Laboratoire d’Histologie I, Faculté de MédecineMarseille Cedex 4France

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