Binding, Uptake, and Catabolism of Low Density (LDL) and High Density Lipoproteins (HDL) by Cultured Smooth Muscle Cells

  • D. Steinberg
  • T. E. Carew
  • D. B. Weinstein
  • T. Koschinsky
Conference paper

Abstract

Over the past several years our laboratory has concerned itself with the sites of removal and degradation of plasma low density lipoproteins (LDL) and the cellular mechanisms involved. In vivo studies in dogs and swine showed that the disappearance of 125I-LDL from the plasma compartment was biphasic, indicating exchange with extravascular pool(s) of LDL (19, 20). Analysis of tissues at the time of sacrifice showed that the liver contained by far the highest concentration of protein radioactivity. The total in the liver at sacrifice was a relatively constant fraction of the 125I-LDL remaining in the plasma at the time of sacrifice (12 – 19 % at intervals of 8 –s 1 22 h after injection of 125I-LDL). Kinetic analysis of the total disappearance curve (14) allowed a calculation of the total extravascular pool size, which amounted to about 21 % of the plasma pool size. Thus by far the largest fraction of the extravascular pool was accounted for in the liver.

Keywords

Cholesterol Glycerol Iodide Trypsin Stein 

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References

  1. 1.
    BIERMAN, E.L., ALBERS, J.J.: Biochim. biophys. Acta 388, 198–202 (1975).PubMedGoogle Scholar
  2. 2.
    BIERMAN, E.L., STEIN, O., STEIN, Y. : Circulat. Res. 35, 136–150 (1974).PubMedGoogle Scholar
  3. 3.
    . BROWN, M.S., GOLDSTEIN, J.L.: Proc. nat. Acad. Sci. (Wash.) 71., 788–792 (1974).CrossRefGoogle Scholar
  4. 4.
    BROWN, M.S., DANA, S.E., GOLDSTEIN, J.L.: J. biol. Chem. 249, 789–796 (1974).PubMedGoogle Scholar
  5. 5.
    CAREW, T.E., KOSCHINSKY, T., STEINBERG, D. : Circulation 52., Suppl. II, 59 (1975)Google Scholar
  6. 6.
    GLOMSET, J.A.: J. Lipid Res. 9., 155–167 (1968).PubMedGoogle Scholar
  7. 7.
    GOLDSTEIN, J.L., BROWN, M.S.: Proc. nat. Acad. Sci. (Wash.) 70, 2804–2808 (1973).CrossRefGoogle Scholar
  8. 8.
    GOLDSTEIN, J.L., BROWN, M.S.: J. biol. Chem. 249, 5153–5162 (1974).PubMedGoogle Scholar
  9. 9.
    GOLDSTEIN, J.L., BROWN, M.S.: Arch. Path. (Chicago) 99, 181–184 (1975).Google Scholar
  10. 10.
    GOLDSTEIN, J.L., BRUNSCHEDE, G.Y., BROWN, M.S.: J. biol. Chem. 250, 7854–7862 (1975).PubMedGoogle Scholar
  11. 11.
    KOSCHINSKY, T., CAREW, T.E., STEINBERG, D.: Circulation 52, Suppl. II, 273 (1975).Google Scholar
  12. 12.
    LANGER, T., STROBER, W., LEVY, R.I.: J. clin. Invest. 51, 1528–1536 (1972).PubMedCrossRefGoogle Scholar
  13. 13.
    MAHLEY, R.W., ASSMANN, G., BROWN, B.G., BREWER, H.B., Jr., WEISGRABER, K.H.: Circulation 50, Suppl. III, 70 (1974).Google Scholar
  14. 14.
    MATTHEWS, C.M.E.: Phys. in Med. Biol. 2, 36–53 (1957).CrossRefGoogle Scholar
  15. 15.
    REICHL, D., SIMONS, L.A., MYANT, N.B., PFLUG, J.J., MILLS, G.L.: Clin. Sci. mol. Med. 45, 313–329 (1973).PubMedGoogle Scholar
  16. 16.
    ROSS, R.J.Cell Biol. 50, 172–186 (1971 )Google Scholar
  17. 17.
    SIMONS, L.A., REICHL, D., MYANT, N.B., MANCINI, M.: Atherosclerosis 21, 283–298 (1975).PubMedCrossRefGoogle Scholar
  18. 18.
    SINGER, S.J.: Advanc. Immunol. 19, 1–66 (1974).CrossRefGoogle Scholar
  19. 19.
    SNIDERMAN, A.D., CAREW, T.E., STEINBERG, D. J. Lipid Res. 16, 293–299 (1975)Google Scholar
  20. 20.
    SNIDERMAN, A.D., CAREW, T.E., CHANDLER, J.G., STEINBERG, D.: Science 183, 526–528 (1974).PubMedCrossRefGoogle Scholar
  21. 21.
    STEIN, O., STEIN, Y.: Biochim. biophys. Acta 326, 232–244 (1973).PubMedGoogle Scholar
  22. 22.
    STEIN, Y., GLANGEAUD, M.C., FAINARU, M., STEIN, O.: Biochim. biophys. Acta 380, 106–118 (1975).PubMedGoogle Scholar
  23. 23.
    STEIN, O., WEINSTEIN, D.B., STEIN, Y., STEINBERG, D.: Proc. nat. Acad. Sci. (Wash.) In press (1976).Google Scholar
  24. 24.
    STEINBERG, D., CAREW, T.E., CHANDLER, J.G., SNIDERMAN, A.D.: The role of the liver in metabolism of plasma lipoproteins. In.: Regula tion of Hepatic Metabolism. LUNDQUIST, F., TYGSTRUP, N. (eds.), pp 144–156. Copenhagen: Munksgaard 1974.Google Scholar
  25. 25.
    WEINSTEIN, D.B., CAREW, T.E., STEINBERG, D.: Circulation 50, Suppl III, 70 (1974).Google Scholar
  26. 26.
    WEINSTEIN, D.B., CAREW, T.E., STEINBERG, D.: Biochim. biophys. Acta. In press (1975).Google Scholar
  27. 27.
    WILLIAMS, C.D., AVIGAN, J.: Biochim. biophys. Acta 260, 413–423 (1972).PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1976

Authors and Affiliations

  • D. Steinberg
  • T. E. Carew
  • D. B. Weinstein
  • T. Koschinsky

There are no affiliations available

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