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Notions About Human Stem Cells

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Book cover Hemopoietic Dysplasias (Preleukemic States)

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Abstract

From an analysis of the known red cell and granulocytic cell turnover rates in the peripheral blood, absolute bone marrow cellularity of man, mitotic index of human marrow, tritiated thymidine labeling index of human marrow, the proportional distribution of the different cell types in the bone marrow of man and the measured DNA synthesis time of differentiated cell lines in human bone marrow, the flux of stem cells into the differentiated compartment has been calculated. By assuming that the same fraction of committed stem cells of man and mouse are in DNA synthesis, the number of cells in DNA synthesis and the size of the stem cell compartments have been calculated. The calculation gives an abundance of stem cells in human marrow that is much larger than that estimated by in vitro culture techniques. On the basis of the calculations, it is hypothesized that the pool of cells preceding the cytologically differentiated pools may consist of self replicating committed stem cells and differentiated cells undergoing amplification that have not developed cytologic markers characteristic of differentiated cells.

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References

  1. CRONKITE, E.P.: Hemopoietic stem cells: an analytical review of hemopoiesis. In: Pathobiology Annuals 1975, HARRY IOACHIM, Editor, Appleton Century Croft, Publisher, N.Y. 1975

    Google Scholar 

  2. BECKER, AJ., MCCULLOCH, E.A., SIMINOVITCH, L., TILL, J.E.: The effect of differing demands for blood cell production on DNA synthesis by hemopoietic colony-forming cells of mice. Blood 26, 296, 1965

    PubMed  CAS  Google Scholar 

  3. PLUZNIK, D. H., SACHS, L.: The cloning of normal blast cells in tissue culture. J. Cell. Comp. Physiol. 66, 319, 1965

    Article  CAS  Google Scholar 

  4. BRADLEY, T. R., METCALF, D.: The growth of mouse bone marrow cells in vitro. Austral. J. Exp. Biol. Med. Sci. 44, 287, 1966

    Article  CAS  Google Scholar 

  5. STEPHENSON, J. R., AXELRAD, A.A., MCLEOD, D.L., SHREEVE, M.M.: Induction of colonies of hemoglobin-synthesizing cells by erythropoietin in vitro. Proc. Nat. Acad. Sci. 68, 1542, 1971

    Article  PubMed  CAS  Google Scholar 

  6. Till, J.E., Mcculloch, E.A.: The “f-factor” of the spleen colony assay for hemopoietic stem cells. Series Hematol. 5, 15, 1972

    CAS  Google Scholar 

  7. VASSORT, F., WINTERHOLER, M., FRINDEL, E., TUBIANA, M.: Kinetic parameters of bone marrow stem cells using in vivo suicide by tritiated thymidine or by hydroxyurea. Blood 41, 789,1973

    PubMed  CAS  Google Scholar 

  8. METCALF, D., MOORE, M.A.S., SHORTMAN, K.: Adherence column and buoyant density separation of bone marrow stem cells and more differentiated cells. J. Cell Physiol. 78, 441,1971

    Article  PubMed  CAS  Google Scholar 

  9. METCALF, D.: Effect of thymidine suiciding on colony formation in vitro by mouse hematopoietic cells. Proc. Soc. Exp. Biol. Med. 139, 511, 1972

    PubMed  CAS  Google Scholar 

  10. ISCOVE, N.N., TILL, J.E., MCCULLOCH, E. A.: The proliferative states of mouse granulopoietic progenitor cells. Proc. Soc. Exp. Biol. Med. 134, 33, 1970

    PubMed  CAS  Google Scholar 

  11. SENN, J.S., MCCULLOCH, E.A.: Radiation sensitivity of human bone marrow cells measured by a cell culture method. Blood 35, 56,1970

    PubMed  CAS  Google Scholar 

  12. MOORE, M.A.S., WILLIAMS, N.: Functional, morphologic, and kinetic analysis of the granulocytemacrophage progenitor cell. In: Hemopoiesis in Culture, W.A. ROBINSON, Editor, p. 17, DHEW Publication No (NIH) 740205, Bethesda, 1973

    Google Scholar 

  13. BRADLEY, T.R., SUMNER, M. A., MCINERNEY, E.: Observations of mouse marrow cell proliferation by erythrocyte extracts. In: Hemopoiesis in Culture, W.A. ROBINSON, Editor, pp. 77–89, DHEW Publication No (NIH) 740205, Bethesda, 1973

    Google Scholar 

  14. ISCOVE, N., SIEBER, F., WINTERHALTER, K.H.: Human urinary erythroid colony stimulating activity: assay in culture of mouse and human marrow and similarity to erythropoietin. In: Hemopoìesis in Culture, W.A. Robinson, Editor, pp.44–73, DHEW Publication No (NIH) 74205, Bethesda, 1973

    Google Scholar 

  15. CARTWRIGHT, G.E., ATHENS, J.W., WINTROBE, M.M.: The kinetics of granulopoiesis in normal man. Blood 24, 780, 1964

    PubMed  CAS  Google Scholar 

  16. CRONKITE, E.P., VINCENT, P.C.: Granulocytopoiesis. Ser. Haemat. Vol. II, (4) 3-43, 1969

    Google Scholar 

  17. DONOHUE, D.M., REIFF, R. H., HANSON, M.L., BETSON, Y., FINCH, C.A.: Quantitative measurements of the erythrocytic and granulocytic cells of the marrow and blood. J. Clin. Invest. 37, 1571, 1958

    Article  PubMed  CAS  Google Scholar 

  18. DONOHUE, D.M., GABRIO, B.W., FINCH, C. A.: Quantitative measurement of hematopoietic cells of the marrow. J. Clin. Invest. 37, 1564, 1958

    Article  PubMed  CAS  Google Scholar 

  19. ODARTCHENKO, N., COTTIER, H., FEINENDEGEN, L.E., BOND, V. P.: Evaluation of mitotic time in vivo using tritiated thymidine as a cell marker: Successive labeling with time of separate mitotic phases. Exp. Cell. Res. 35, 402, 1964

    Article  PubMed  CAS  Google Scholar 

  20. KILLMANN, S.A., CRONKITE, E. P., FLIEDNER, T.M., BOND, V.P.: Mitotic index of human bone marrow cells. 1. Number and cytologic distribution of mitoses. Blood 19, 743, 1962

    PubMed  CAS  Google Scholar 

  21. MAUER, A.M.:Diurnal variation of proliferative activity in the human bone marrow. Blood 26, 1, 1965

    PubMed  CAS  Google Scholar 

  22. STRYCKMANS, P., CRONKITE, E.P., FLIEDNER, T.M., RAMOS, J.: DNA synthesis time of erythropoietic and granulopoietic cells in human beings. Nature 211, 717, 1966

    Article  PubMed  CAS  Google Scholar 

  23. WICKRAMINSINGHE, S.N., MOFFATT, B.: Observations on cell proliferation in human myelocytes. Acta Haematol. 46, 193, 1971

    Article  Google Scholar 

  24. HAYFLICK, L., MOORHEAD.: The serial cultivation of human diploid cells. Exp. Cell Res. 25, 585, 1961

    Google Scholar 

  25. HAYFLICK, L., MOORHEAD.: The serial cultivation of human diploid cells. Exp. Cell Res. 25, 585, 1961

    Google Scholar 

  26. KAY, H.E.M.: How many cell generations? Lancet ii, 418,1965

    Article  Google Scholar 

  27. THOMAS, E.D., FLIEDNER, T.M., THOMAS, D., CRONKITE, E.P.: The problems of the stem cell: observations in dogs following nitrogen mustard. J. Lab. Clin. Med. 65, 794,1965

    PubMed  CAS  Google Scholar 

  28. VAN BEKKUM, D.W., VAN NOORD, M.J., MAAT, B., DICKE, K.A.: Attempts at identification of hemopoietic stem cell in mouse. Blood 38, 547, 1971

    PubMed  Google Scholar 

  29. TYLER, R. W. C., EVERETT, N. B.: Radioautographic study of hemopoietic repopulation using irradiated parabiotic rats — relation to stem cell problem. Blood 28, 873, 1966

    Google Scholar 

  30. ZUCKER-FRANKLIN, D., GRUSKY, G., L’ESPERANCE, P.: Granulocyte colonies derived from lymphocyte fractions of normal human peripheral blood. Proc. Nat. Acad. Sci. USA 71, 2711, 1974

    Article  PubMed  CAS  Google Scholar 

  31. BARR, R.D., WHANG-PENG, J., PERRY, S.: Hemopoietic stem cells in human peripheral blood. Science 190, 284, 1975

    Article  PubMed  CAS  Google Scholar 

  32. BESSIS, M.: Living Blood Cells and Their Ultrastructure, Springer-Verlag, Berlin, 1973

    Google Scholar 

  33. WINTROBE, M.: Clinical Hematology, Fifth Edition, Lea and Febiger, Publishers, Philadelphia 1961

    Google Scholar 

  34. Osmond, D.G., EVERETT, N. B.: Radioautographic studies of bone marrow lymphocytes in vivo and in diffusion chamber cultures. Blood 23, (1) 1, 1964

    PubMed  CAS  Google Scholar 

  35. KEISER, G., COTTIER, H., ODARTCHENKO, N., BOND, V.P.: Autoradiographic study on the origin and fate of small lymphoid cells in the dog bone marrow: effect of femoral artery clamping during in vivo availability of H3-thumidine. Blood 24, 254,1964

    PubMed  CAS  Google Scholar 

  36. ROSSE, C.: Lymphocyte production and life-span in the bone marrow of the guinea pig. Blood 38, 372, 1971

    PubMed  CAS  Google Scholar 

  37. OSMOND, D.G., MILLER, S.C., YOSHIDA, Y.: Kinetic and haemopoietic properties of lymphoid cells in the bone marrow. In: Haemopoietic Stem Cells, Ciba Foundation Symposium 13, pp. 131–156, Elsevier, Amsterdam, 1973

    Google Scholar 

  38. BOGGS, D.R.: The kinetics of neurophilic leukocytes in health and in disease. Seminars in Hematol. 4, 359, 1967

    CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Medical Research Center, Brookhaven National Laboratory, Upton, New York, 11973, USA

    Eugene P. Cronkite

  2. Institute of Medicine, Kernforschungsanlage, D-5170, Jülich, Federal Republic of Germany

    Ludwig E. Feinendegen

Authors
  1. Eugene P. Cronkite
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  2. Ludwig E. Feinendegen
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Editor information

Marcel Bessis George Brecher

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© 1977 Springer-Verlag Berlin Heidelberg

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Cronkite, E.P., Feinendegen, L.E. (1977). Notions About Human Stem Cells. In: Bessis, M., Brecher, G. (eds) Hemopoietic Dysplasias (Preleukemic States). Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66312-3_18

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  • DOI: https://doi.org/10.1007/978-3-642-66312-3_18

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-07597-4

  • Online ISBN: 978-3-642-66312-3

  • eBook Packages: Springer Book Archive

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