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Abnormal Distribution of Erythrocytes A1 Antigens in Preleukemia as Demonstrated by an Immunofluorescence Technique

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Hemopoietic Dysplasias (Preleukemic States)

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Abstract

Acquired changes of erythrocyte agglutinability, especially that of A1 blood group, are a conspicuous finding in preleukemic states.

Evidence of several populations of cells has already been provided by separation procedures using specific agglutination methods. Another kind of direct evidence for heterogeneity is given by a membrane labeling method using an indirect immunofluorescence technique. Red cells were sensitized by the lectin of Dolichos biflorus which is known to possess a combining specific A1 receptor. Specific vizualisation was carried out using fluorescein-conjugated antilectin elicited from rabbits.

Twelve samples from blood group A1 patients with preleukemia were studied. The fluorescence pattern of each sample was compared with its agglutinability figure. An abnormal pattern of fluorescence was commonly observed, such as a marked reduction in fluorescent cells, but a mixture of variously stained cells always persisted. This heterogeneous distribution of cells carrying variable amounts of A1 receptors may reflect an exaggeration of physiologic heterogeneity. It was demonstrated to be not associated with cell aging. The significance of these findings is thus discussed at the molecular level ascribing the reduction in the antigenic cell load to a disturbed genetic regulation rather than to an impairment in structural genes responsible for blood group transferase syntheses.

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Authors and Affiliations

  1. Unité de Recherches sur les Anémies, INSERM U.91. Hôpital Henri Mondor, F-94010, Creteil, France

    H. Rochant, H. Tonthat, A. Henri, M. Titeux & B. Dreyfus

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  1. H. Rochant
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  2. H. Tonthat
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  3. A. Henri
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  4. M. Titeux
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  5. B. Dreyfus
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Marcel Bessis George Brecher

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© 1977 Springer-Verlag Berlin Heidelberg

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Rochant, H., Tonthat, H., Henri, A., Titeux, M., Dreyfus, B. (1977). Abnormal Distribution of Erythrocytes A1 Antigens in Preleukemia as Demonstrated by an Immunofluorescence Technique. In: Bessis, M., Brecher, G. (eds) Hemopoietic Dysplasias (Preleukemic States). Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66312-3_16

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  • DOI: https://doi.org/10.1007/978-3-642-66312-3_16

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-07597-4

  • Online ISBN: 978-3-642-66312-3

  • eBook Packages: Springer Book Archive

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