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Growth of Biotrophic Parasites in Axenic Culture

  • K. J. Scott
Part of the Encyclopedia of Plant Physiology book series (PLANT, volume 4)

Abstract

Pathogens of plants can be divided into two broad categories: biotrophic and necrotrophic parasites. These terms were originally used by Link (1933) to describe the methods by which pathogens obtain nutrients from host cells under natural conditions. Necrotrophic parasites derive their nutrients from dead host cells, usually killed by the parasite in advance of penetration. Biotrophic parasites derive their nutrients from living host cells. This group includes both facultative saprophytes and obligate parasites.

Keywords

Powdery Mildew Downy Mildew Germ Tube Rust Fungus Axenic Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Allen, R.F.: A cytological study of Puccinia triticina physiologic form II on Little Club wheat. J. Agr. Res. 33, 201–222 (1926).Google Scholar
  2. Bary, A. de: Comparative Morphology and Biology of the Fungi, Mycetozoa and Bacteria. Eng. trans, by Garnsey, H.E.F., rev. by Balfour, I.B., Oxford: Clarendon Press (1887).Google Scholar
  3. Bose, A., Shaw, M.: Sporulation and pathogenicity of an Australian isolate of wheat rust grown in vitro. Canad. J. Botany. 49, 1961–1964 (1971).CrossRefGoogle Scholar
  4. Bose, A., Shaw, M.: The axenic culture of Melampsora lini (flax rust). Abstract 2nd Int. Cong Plant Pathol 1002 (1973).Google Scholar
  5. Brian, P.W.: Obligate parasitism in fungi. Proc. Roy. Soc. (London), Ser. B 168, 101–118 (1967).CrossRefGoogle Scholar
  6. Brown, W.: The physiology of host-parasite relations. Botan. Rev. 2, 236–281 (1936).CrossRefGoogle Scholar
  7. Bushnell, W.R.: In vitro development of an Australian isolate of Puccinia graminis f. sp. tritici. Phytopathology 58, 526–527 (1968).Google Scholar
  8. Bushnell, W.R., Rajendren, R.B.: Casein hydrolysates and peptones for artificial culture of Puccinia graminis f. sp. tritici. Phytopathology 60, 1287 (Abstr.) (1970).CrossRefGoogle Scholar
  9. Bushnell, W.R., Stewart, D.M.: Development of American isolates of Puccinia graminis f. sp. tritici on an artificial medium. Phytopathology 61, 376–379 (1971).CrossRefGoogle Scholar
  10. Calavan, E.C., Igwegbe, E.C.K., Fudl-Allah, A.E.: Recent developments in the etiology and control of stubborn disease of citrus. Proc. 2nd Int. Symp. Plant Pathol., New Delhi (Abstr.): 132–133 (1971).Google Scholar
  11. Chen, T.A.,Granados, R.R.: Plant pathogenic mycoplasma-like organisms: maintenance in vitro and transmission to Zea mays Life Science 167, 1633–1636 (1970).Google Scholar
  12. Coffey, M.D.,Allen, P.J.: Nutrition of Melampsora lini and Puccinia helianthi. Trans. Brit. Mycol. Soc. 60, 245–260 (1973).CrossRefGoogle Scholar
  13. Coffey, M.D., Bose, A., Shaw, M.: In vitro growth of gelatin suspensions of uredospores of Puccinia graminis f. sp. tritici. Canad. J. Botany 47, 1291–1293 (1969).CrossRefGoogle Scholar
  14. Coffey,M.D., Bose, A., Shaw, M.: In vitro culture of the flax rust, Melampsora lini. Canad. J. Botany 48, 773–776 (1970).CrossRefGoogle Scholar
  15. Coffey, M.D., Shaw, M.: Nutritional studies with axenic cultures of the flax rust, Melampsora lini. Physiol. Plant Pathol. 2, 37–46 (1972).CrossRefGoogle Scholar
  16. Daniels, M.J., Parkham, P.G., Meddins, B.M., Plaskitt, A.K., Townsend, R., Bar-Joseph, M.: Axenic culture of a plant pathogenic spiroplasma. Nature 244, 523–524 (1973).PubMedCrossRefGoogle Scholar
  17. Day, P.R., Anagnostakis, S.L.: Corn smut dikaryon in culture. Nature New Biol. 231, 19–20 (1971).PubMedCrossRefGoogle Scholar
  18. Dickinson, S.: Studies in the physiology of obligate parasitism. II. Behaviour of the germ tubes of certain rusts in contact with various membranes. Ann. Botany (N.S.) 13, 219–236 (1949).Google Scholar
  19. Dougherty, E.C.: Problems of nomenclature for the growth of organisms of one species with and without associated organisms of other species. Parasitology 42, 259–261 (1953).PubMedCrossRefGoogle Scholar
  20. Faivre-Amiot, A., Moreau, J.P., Cousin,M.T., Staron, T.: Essai de mise en culture de l’agent de la Phyllodie du Trefle. Ann. Phytopathol. 2, 251–258 (1970).Google Scholar
  21. Foudin, A.S., Wynn, W.K.: Growth of Puccinia graminis f. sp. tritici on a defined medium. Phytopathology 62, 1032–1040 (1972).CrossRefGoogle Scholar
  22. Fromme, F.D.: The culture of rusts in the glasshouse. Bull. Torrey Botan. Club 40, 501–521 (1913).CrossRefGoogle Scholar
  23. Fuchs, W.H., Gaertner, A.: Untersuchungen zur Keimungsphysiologie des Schwarzrostes Puccinia graminis tritici (pers.) Erikss. u. Henn. Arch. Mikrobiol. 28, 303–309 (1958).CrossRefGoogle Scholar
  24. Fudl-Allah, A.E., Calavan, E.C., Igwegbe,E.C.K.: Culture of a mycoplasma-like organism associated with stubborn disease of citrus. Phytopathology 61, 1321 (Abstr.) (1971).CrossRefGoogle Scholar
  25. Gautheret, R.J.: Manual technique de la culture des tissus vegetaux. Paris: Masson and Cie (1942).Google Scholar
  26. Ghosh, S.K.,Raychaudhuri, S.P., Varma,A., Nariani, T.K.: Isolation and culture of mycoplasma associated with citrus greening disease. Current Sci. (India) 40, 299–300 (1971).Google Scholar
  27. Giannotti, J. Vago, C.: Role des mycoplasmes dans Fetiologie de la phyllodie du trefle: culture et transmission experimentale de la maladie. Physiol. Veg. 9, 541–553 (1971).Google Scholar
  28. Giannotti, J., Vago,C., Leclant, F., Marchoux, G., Czarnecky, D.: Obtention de cultures de mycoplasmes a partir de Lavendula hybrida Reverchon attain te de «deperissement» et de Hyalestes obsoletus Sign., un vecteur probable de la maladie. Compt. Rend. Ser. D.Sci. Nat. (Paris) 274, 394–397 (1972).Google Scholar
  29. Hampton, R.O., Stevens, J.O., Allen,T.C.: Mechanically transmissible Mycoplasma from naturally infected peas. Plant Disease Reptr. 53, 499–503 (1969).Google Scholar
  30. Hanes, C.S., Hird, F.J.R., Isherwood,F.A.: Synthesis of peptides in enzymic reactions involving glutathione. Nature 166, 288–292 (1950).PubMedCrossRefGoogle Scholar
  31. Howes, N.K., Scott, K.J.: Sulphur nutrition of Puccinia graminis f. sp. tritici in axenic culture. Canad. J. Botany 50, 1165–1170 (1972).CrossRefGoogle Scholar
  32. Howes, N.K., Scott, K.J.: Sulphur metabolism of Puccinia graminis f. sp. tritici in axenic culture. J. Gen. Microbiol. 76, 345–354 (1973).PubMedGoogle Scholar
  33. Igwegbe, E.C.K., Calavan, E.C.: Occurrence of mycoplasma-like bodies in phloem of stubborn-infected citrus seedlings. Phytopathology 60, 1525–1526 (1970).CrossRefGoogle Scholar
  34. Jones, D.R.: In vitro culture of carnation rust, Uromyces dianthi. Trans. Brit. Mycol. Soc. 58, 29–36 (1972).CrossRefGoogle Scholar
  35. Jones, D.R.: Growth and nutritional studies with axenic cultures of the carnation rust fungus, Uromyces dianthi (Pers.) Niessl. Physiol. Plant Pathol. 3, 379–386 (1973).CrossRefGoogle Scholar
  36. Kanazawa, A., Katimoto, Y., Nakajima, T., Sano,I.: Identification of γ-glutamylserine, γ-glutamylalanine, γ-glutamylvaline and S-methylglutathione of bovine brain. Biochim. Biophys. Acta 111, 90–95 (1965).PubMedCrossRefGoogle Scholar
  37. Kuhl, J.L., Maclean,D.J., Scott, K.J., Williams, P.G.: The axenic culture of Puccinia species from uredospores: experiments on nutrition and variation. Canad. J. Botany 49, 201–209 (1971).CrossRefGoogle Scholar
  38. Lafleche, D., Bove, J.M.: Structures de type mycoplasme dans les feuilles d’orangers atteints de la maladie du «Greening». Compt. Red. 270, 1915–1917 (1970).Google Scholar
  39. Lane,D.W., Shaw,M.: Axenic culture of flax rust isolated from cotyledons by cell wall digestion. Canad. J. Botany 50, 2601–2603 (1972).CrossRefGoogle Scholar
  40. Lesovoj, M.P., Kondratyuk, O.K., Shelekhova,L.N.: Cultivation of fungus Puccinia recondita Rob. Ex. Desm. f. sp. tritici on the artificial medium. Abstract 2nd Int Cong Plant Pathol 1005 (1973).Google Scholar
  41. Lin, S.C., Lee,C.S., Chiu, R.J.: Isolation and cultivation of, and inoculation with, a Mycoplasma causing white leaf disease of sugarcane. Phytopathology 60, 1087–1103 (1970).CrossRefGoogle Scholar
  42. Link, G.K.K.: Etiological phytopathology. Phytopathology 23, 843–862 (1933).Google Scholar
  43. Lombardo, G., Pignattelli, P.: Cultivation in a cell-free medium of a mycoplasma-like organism from Vinca rosea with phyllody symptoms of the flowers. Ann. Microbiol. 20, 84–89 (1970).Google Scholar
  44. Maclean, D.J.: Studies on axenic cultures of the wheat stem rust fungus. Ph. D. Thesis, Univ. Queensland, Brisbane, Australia. (1972).Google Scholar
  45. Maclean, D.J.: Cultural and nutritional studies on variant strains of the wheat stem rust fungus. Trans. Brit. Mycol. Soc. 62, 333–349 (1974).CrossRefGoogle Scholar
  46. Maclean, D.J., Scott, K.J.: Variant forms of saprophytic mycelium grown from uredospores of Puccinia graminis f. sp. tritici. J. Gen. Microbiol. 64, 19–27 (1970).Google Scholar
  47. Maclean, D.J., Scott, K.J.: Pathogenicity of variant strains of the wheat stem rust fungus isolated from axenic culture. Canad. J. Botany 52, 201–207 (1974).CrossRefGoogle Scholar
  48. Maclean, D.J., Scott, K.J., Tommerup, I.: A uninucleate wheat infecting strain of the stem rust fungus isolated from axenic cultures. J. Gen. Microbiol. 65, 339–342 (1971).Google Scholar
  49. Maclean, D.J., Tommerup, I.C., Scott, K.J.: Genetic status of monokaryotic variants of the wheat stem rust fungus isolated from axenic culture J. Gen. Microbiol. 84, 364–378 (1974).Google Scholar
  50. Mains, E.B.: The relation of some rusts to the physiology of their hosts. Am. J. Botany 4, 179–220 (1917).CrossRefGoogle Scholar
  51. Maramorosch, K.: The enigma of mycoplasma in plants and insects. Lett, to Ed. Phytopathol. 62, 1230–1231 (1972).Google Scholar
  52. Morel, G.: Le developpement du mildiou sur des tissus de vigne cultives in vitro. Compt. Rend. 218, 50 (1944).Google Scholar
  53. Morel, G.: Recherches sur la culture associee de parasites obligatoires et de tissus vegetaux. Ann. Epiphyties (II) 14, 1–112 (1948).Google Scholar
  54. Morris, C.J., Thompson, J.F.: The identification of (+) S-methyl-L-cysteine sulfoxide in plants. J. Amer. Chem. Soc. 78, 1605–1608 (1956).CrossRefGoogle Scholar
  55. Morton, A.G., Broadbent, D.: The formation of extra-cellular nitrogen compounds by fungi. J. Gen. Microbiol. 12, 248–258 (1955).PubMedGoogle Scholar
  56. Naito, N., Matsuka, N.: Infection-type structures produced on artificial media by spores of rust fungi. II. Techn. Bull. Fac. Agric. Kagawa Univ. 17, 8–16 (1965).Google Scholar
  57. Nayar, R.M., Ananthapadmanabha, H.S.: Isolation, cultivation and pathogenicity trials with mycoplasma-like bodies associated with sandal spike disease. J. Indian Acad. Wood Sci. 1, 59–61 (1970).Google Scholar
  58. Orlowski, M., Meister, A.: The γ-glutamyl cycle: a possible transport system for amino acids. Proc. Nat. Acad. Sci. 67, 1248–1255 (1970).PubMedCrossRefGoogle Scholar
  59. Pole-Evans, I.B.: The cereal rusts. I. The development of their uredo mycelia. Ann. Bot. (London) 21, 441–446 (1907).Google Scholar
  60. Robinow, C.F., Caten, C.E.: Mitosis in Aspergillus nidulans. J. Cell Sci. 5, 504–531 (1969).Google Scholar
  61. Saglio, P.,Lafleche, D., Bonissol, C., Bove, J.M.: Isoliment et culture in vitro des mycoplasmes associes au «Stubborn» des agrumes et leur observation au microscope électronique. Compt. Red. Ser. D. Sci. Nat. (Paris) 272, 1387–1390 (1971).Google Scholar
  62. Saglio, P., Lhospital, M., Laflèche, O., Dupont, G., Bove, J.M., Tully, J.G., Freundt, E.A.: Spiroplasma citrigen and sp. n.: a new mycoplasma-like organism associated with “Stubborn” disease of citrus. Int. J. Syst. Bacter. 23, 191–204 (1973).CrossRefGoogle Scholar
  63. Schiff, J.A., Hodson, R.C.: The metabolism of sulphate. Ann. Rev. Plant Physiol. 24, 381–414 (1973).CrossRefGoogle Scholar
  64. Scott, K.J.,Maclean, D.J.: Culturing of rust fungi. Ann. Rev. Phytopathol. 7, 123–146 (1969).CrossRefGoogle Scholar
  65. Singleton, L.L., Young, H.C.: The in vitro culture of Puccinia recondita f. sp. tritici. Phytopathology, 58: 1068 (Abstr.) (1968).Google Scholar
  66. Tiwari, M.M., Arya,H.C: Sclerospora graminicola axenic culture. Science 163, 291–293 (1969).PubMedCrossRefGoogle Scholar
  67. Tkachuk, R.: L-Cysteinylglycine: its occurrence and identification. Canad. J. Biochem. 48, 1029–1036 (1970).CrossRefGoogle Scholar
  68. Turel, F.L.M.: Saprophytic development of the flax rust Melampsora lini race No. 3. Canad. J. Botany 47, 821–823 (1969a).CrossRefGoogle Scholar
  69. Turel, F.L.M.: Low temperature requirements for saprophytic flax rust cultures. Canad. J. Botany 47, 1637–1638 (1969b).CrossRefGoogle Scholar
  70. Turel, F.L.M.: Pathogenicity and developmental differences of three saprophytically growing isolates of the flax rust fungus Melampsora lini, race 3. Canad. J. Botany 49, 1993–1997 (1971).CrossRefGoogle Scholar
  71. Turel, F.L.M.: Histology of infection and sporulation by saprophytic cultures of Melampsora lini inoculated onto flax cotyledons in sterile culture. Canad. J. Botany 50, 227–230 (1972).CrossRefGoogle Scholar
  72. Turel,F.L.M.: Growth of the flax rust Melampsora lini on chemically defined media. Canad. J. Botany 51, 131–134 (1973).CrossRefGoogle Scholar
  73. Waelisch, H.: Certain aspects of intermediary metabolism of glutamine, asparagine and glutathione. Advan. Enzymol. 13, 237–319 (1952).Google Scholar
  74. White, P.R.: A Handbook of Plant Tissue Culture. Lancaster, Pennsylvania: Jacques Cattell Press (1943).Google Scholar
  75. Williams, P.G.: A new perspective of the axenic culture of Puccinia graminis f. sp. tritici from uredospores. Phytopathology 61, 994–1002 (1971).CrossRefGoogle Scholar
  76. Williams, P.G.,Hartley, M.J.: Occurrence of diploid lines of Puccinia graminis tritici in axenic culture. Nature 229, 181–182 (1971).Google Scholar
  77. Williams, P.G., Scott,K.J., Kuhl, J.L.: Vegetative growth of Puccinia graminis f. sp. tritici in vitro. Phytopathology 56 (12), 1418–1419 (1966).Google Scholar
  78. Williams, P.G., Scott, K.J., Kühl, J.L., Maclean, D.J.: Sporulation and pathogenicity of Puccinia graminis f. sp. tritici grown on an artificial medium. Phytopathology 57, 326–327 (1967).Google Scholar
  79. Wong, A.L., Willetts, H.J.: Observations on the growth of selected Australian races of wheat stem rust in axenic culture. Trans. Brit. Mycol. Soc. 55, 231–238 (1970).CrossRefGoogle Scholar
  80. Yarwood, C.E.: Obligate parasitism. Ann. Rev. Plant Physiol. 7, 115–142 (1956).CrossRefGoogle Scholar

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© Springer-Verlag Berlin · Heidelberg 1976

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  • K. J. Scott

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