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Antigen-Binding Properties of Antibody Molecules: Time-Course Dynamics and Biological Significance

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Book cover Current Topics in Microbiology and Immunology / Ergebnisse der Mikrobiologie und Immunitätsforschung

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Abstract

Injection of an immunogen into a susceptible, immunocompetent animal, elicits production of antibody molecules specific to the antigenic determinants of the immunogen. If the immunogen possesses different determinants the corresponding antibody species are synthesized, thus in the case of complex immunogens the response is made up of a mixture of several antibody populations with different specificities. These antibody populations appear simultaneously or successively. Within each specificity subpopulations of molecules are often found differing in the strength with which the antigen-combining site binds to the corresponding determinant. The binding properties as well as other characteristics of antibodies, such as immunoglobulin class, allotype, and idiotype usually change with time after immunization. We shall review data on these chronological changes as a means of looking into the time-course dynamics of antibody-forming clones. Most of the information available refers to antibodies of IgG class and, to a lesser extent, to antibodies of IgM class, purposely induced by injecting experimental antigens. Data on the binding properties of other Ig classes are scarce. For IgA some information exists which was obtained using myeloma proteins; we shall consider it only marginally.

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Abbreviations

K0 :

equilibrium constant

ΔF:

free energy change

K·cal/mole:

kilo calory · mole-1

α or a:

heterogeneity index

Ig:

immunoglobulin

Fab:

fragment of immunoglobulin bearing the antigen-combining site

DNP:

dinitrophenyl

AFCP:

antibody-forming cell precursor

NIP:

4-hydroxy-3-iodo-5-phenylacetic acid

a.a.:

aminoacid

IEF:

isoelectric focusing

pI:

isoelectric point

CD:

circular dichroism

PCA:

passive cutaneous anafylaxis

C’:

complement

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Authors and Affiliations

  1. Division of Biological and Medical Sciences, Brown University, Providence, Rhode Island, 02912, USA

    Everly Conway de Macario

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  1. Alberto J. L. Macario
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  2. Everly Conway de Macario
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Editors and Affiliations

  1. Basle, Switzerland

    W. Arber  & N. K. Jerne  & 

  2. Philadelphia, USA

    W. Henle , P. Koldovský  & H. Koprowski ,  & 

  3. Martinsried, Germany

    P. H. Hofschneider

  4. London, UK

    J. H. Humphrey

  5. Copenhagen, Denmark

    O. Maaløe

  6. Gießen, Germany

    R. Rott

  7. Wilhelmshaven, Germany

    H. G. Schweiger

  8. Rehovot, Israel

    M. Sela

  9. Prague, Czech Republic

    L. Syruček

  10. Seattle, USA

    P. K. Vogt

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© 1975 Springer-Verlag, Berlin · Heidelberg

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Macario, A.J.L., de Macario, E.C. (1975). Antigen-Binding Properties of Antibody Molecules: Time-Course Dynamics and Biological Significance. In: Arber, W., et al. Current Topics in Microbiology and Immunology / Ergebnisse der Mikrobiologie und Immunitätsforschung. Current Topics in Microbiology and Immunology / Ergebnisse der Mikrobiologie und Immunitätsforschung, vol 71. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66193-8_4

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  • DOI: https://doi.org/10.1007/978-3-642-66193-8_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-66195-2

  • Online ISBN: 978-3-642-66193-8

  • eBook Packages: Springer Book Archive

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