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Formation of Cerebrospinal Fluid

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Abstract

In 1972, our laboratory developed a method in which the choroid plexus of the sheep could be maintained satisfactorily in an extracorporeal perfusion system for up to seven hours. The experimental system has been presented elsewhere in great detail (1) and will only be briefly described at this time. Arterial inflow to the plexus was controlled by means of a catheter in the internal carotid artery with ligation of all branches except the anterior choroidal artery. Venous outflow from the plexus was collected from a catheter in the internal cerebral vein at the level of the foramen of Monro. In some fifty experiments, we calculated a rate of CSF production of almost 13µ1 min−1 when the perfusion pressure and choroidal artery blood flow was 90–100mmHg and 2.9µl mg−1 min−1, respectively. The virtual concentration of sodium and chloride in the nascent fluid was computed from the net flux of each ion divided by the net flux of water. The concentration for sodium in the nascent CSF was 158 meq/l and 127.9 meq/l of chloride. The ratio of the concentration in CSF/concentration in plasma was found in both cases to be greater than that expected of a simple ultrafiltrate of plasma.

Keywords

  • Adenosine Triphosphatase
  • Sodium Pump
  • Secretory Epithelium
  • Hypotonic Medium
  • Anterior Choroidal Artery

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Supported in part by PHS Grant NS09002

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References

  1. POLLAY, M., STEVENS, A., ESTRADA, E., KAPLAN, R.: Extracorporeal perfusion of choroid plexus. J. Appl. Physiol. 32, 612–617 (1972).

    PubMed  CAS  Google Scholar 

  2. WELCH, K.: Secretion of cerebrospinal fluid by choroid plexus of the rabbit. Am. J. Physiol. 205, 617–624 (1963).

    PubMed  CAS  Google Scholar 

  3. MILHORAT, T.H.: Choroid plexus and cerebrospinal fluid production. Science 166, 1514–1516 (1969).

    PubMed  CrossRef  CAS  Google Scholar 

  4. AMES, A., HIGASHI, K., NESBETT, F.B.: Effects of PCO2, acetazolamide and ouabain on volume and composition of choroidplexus fluid. J. Physiol. 181, 516–524 (1965).

    PubMed  Google Scholar 

  5. CSERR, H.F.: Physiology of the choroid plexus. Physiol. Rev. 51, 273–311 (1971).

    PubMed  CAS  Google Scholar 

  6. DAVSON, H., SEGAL, M.B.: The effects of some inhibitors and 22 accelerators of sodium transport on the turnover of Na in the cerebrospinal fluid and the brain. J. Physiol. 209, 131–153 (1970).

    PubMed  CAS  Google Scholar 

  7. VATES, T.S., JR., BONTING, S.L., OPPELT, W.W.: Na-K activated adenosine triphosphatase formation of cerebrospinal fluid in the cat. Am. J. Physiol. 206, 1165–1172 (1964).

    PubMed  CAS  Google Scholar 

  8. QUINTON, P.M., WRIGHT, E.M., MCD. TORMEY, J.: Localization of sodium pumps in the choroid plexus epithelium. J. Cell Biol. 58, 724–730 (1973).

    PubMed  CrossRef  CAS  Google Scholar 

  9. PATLAK, CS., GOLDSTEIN, D.A., HOFFMAN, J.F.: The flow of solute and solvent across a two membrane system. J. Theoret. Biol. 5, 426–442 (1963).

    CrossRef  CAS  Google Scholar 

  10. OSCHMAN, J.L., BERRIDGE, M.J.: The structural basis of fluid secretion. Fed. Proc. 30, 49–56 (1971).

    PubMed  CAS  Google Scholar 

  11. DIAMOND, J.M., BOSSERT, W.H.: Standing gradient osmotic flow. A mechanism for coupling of water and solute transport in epithelia. J. Gen. Physiol. 50, 2061–2083 (1967).

    PubMed  CrossRef  CAS  Google Scholar 

  12. DOHRMANN, G.J.: The choroid plexus: A historical review. Brain Res. 18, 197–218 (1970).

    PubMed  CrossRef  CAS  Google Scholar 

  13. BURGESS, A., SEGAL, M.B.: Morphological changes associated with inhibition of fluid transport in the rabbit choroid plexus. J. Physiol. 208, 88–91 (1970).

    Google Scholar 

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© 1975 Springer-Verlag Berlin Heidelberg

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Pollay, M. (1975). Formation of Cerebrospinal Fluid. In: Lundberg, N., Pontén, U., Brock, M. (eds) Intracranial Pressure II. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66086-3_5

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  • DOI: https://doi.org/10.1007/978-3-642-66086-3_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-66088-7

  • Online ISBN: 978-3-642-66086-3

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