Die Stoffwechselwirkungen des Glukagon

  • Viviane Leclercq-Meyer
  • Willy J. Malaisse
Part of the Handbuch der inneren Medizin book series (INNEREN, volume 7 / 2 / A)

Zusammenfassung

Glukagon, der hyperglykämisierende glykogenolytische Faktor des Pankreas, wurde fast zur selben Zeit wie das Insulin entdeckt, nämlich vor ungefähr 5 Jahrzehnten. Kimball und Murlin (1923) trennten als erste eine hyperglykämisierende Substanz aus Insulinpräparaten ab, die sie Glukagon nannten. Neben vielen anderen haben de Duve (1953) und Bergen und van Itallie (1960) die Geschichte der Glukagonforschung ausführlich beschrieben. Während der 20 Jahre, die seiner Entdeckung folgten, kamen nur wenig neue Erkenntnisse über das Glukagon hinzu. Manche Forscher, wie Bürger u. Brandt (1935) glaubten an seine Existenz und versuchten, das Glukagon in reiner Form darzustellen. Demgegenüber schrieben andere die hyperglykämisierenden und glykogenolytischen Wirkungen der Pankreasextrakte und einiger Insulinpräparate dem Insulin selbst oder der Anwesenheit von heterogenen Verunreinigungen zu. Erst in den fünfziger Jahren trat die Arbeit über das Glukagon in eine neue Phase, als Sutherland u. de Duve (1948) und Sutherland et al. (1949) den hyperglykämisierenden Faktor aus dem Pankreas extrahierten und begannen, seine Eigenschaften zu untersuchen. Seitdem ist eine Fülle von Informationen über die verschiedenen metabolischen Wirkungen und den Wirkungsmechanismus dieses Peptids gesammelt worden. Trotzdem war es lange Zeit schwierig, die Stellung des Glukagon als Hormon und seine physiologische Rolle zu definieren, insbesondere beim Menschen. Dies hing hauptsächlich mit der Tatsache zusammen, daß bis vor kurzem kein definierter pathologischer Zustand einem Überschuß oder einem Defekt der Glukagonsekretion zugeordnet werden konnte.

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Literatur

  1. Aguilar-Parada, E., Eisentraut, A.M., Unger, R.H.: Effects of starvation on plasma pancreatic glucagon in normal man. Diabetes 18, 717–723(1969a).Google Scholar
  2. Aguilar-Parada, E., Eisentraut, A.M., Unger, R.H.: Pancreatic glucagon secretion in normal and diabetic subjects. Amer. J. med. Sci. 257, 415–418(1969b).Google Scholar
  3. Assan, R., Boillot, J.: Pancreatic glucagon and glucagon-like material in tissues and plasma from human fetuses 6-26 weeks old. Path. Biol. 21, 149–155(1973).Google Scholar
  4. Assan, R., Boillot, J., Attali, J.R., Soufflet, E., Ballero, G.: Diphasic glucagon release induced by arginine in the perfused rat pancreas. Nature (Lond.) New Biol. 239, 125–126(1972).Google Scholar
  5. Assan, R., Hautecouverture, G., Guillemant, S., Dauchy, F., Protin, P., Derot, M.: Evolution de parametres hormonaux (glucagon, Cortisol, hormone somatotrope) et energetiques (glucose, acides gras, glycerol libre) dans dix acido-cetoses diabetiques graves traitees. Path. et Biol. 17, 1095–1105(1969a).Google Scholar
  6. Assan, R., Rosselin, G., Dolais, J.: Effets sur la glucagonemie des perfusions et ingestions d’acides amines. Journees Annuelles de Diabetologie de l’Hotel Dieu, p. 25–41. Paris: Editions Medicales Flammarion 1967.Google Scholar
  7. Assan, R., Rosselin, G., Drouet, J., Dolais, J., Tchobroutsky, G.: Glucagon antibodies. Lancet 1965II, 590–591.Google Scholar
  8. Assan, R., Rosselin, G., Drouet, J., Dolais, J., Tchobroutsky, G., Derot, M.: Dosage radioimmunologique du glucagon plasmatique chez l’homme. Resultats preliminaries. Ann. Endocr. (Paris) 27, 690–695(1966).Google Scholar
  9. Assan, R., Tchobroutsky, G., Rosselin, G.: Caracterisation radioimmunologique de glucagon dans les tissus digestifs de diverses especes animales. Path. et Biol. 17, 747–755(1969b).Google Scholar
  10. Assan, R., Tiengo, A.: Comparaison des secretions de glucagon dans les diabetes sucres avec ou sans pancreatopathie organique acquise. Path. et Biol. 21, 17–25(1973).Google Scholar
  11. Balasse, E. O.: Regulation du metabolisme extra-hepatique des corps cetoniques in vivo. Bruxelles: Thesis, 1971.Google Scholar
  12. Baum, J., Simons, B.E., Unger, R.H., Madison, L.L.: Localization of glucagon in the alpha cells in the pancreatic islet by immunofluorescent technics. Diabetes 11, 371–374(1962).PubMedGoogle Scholar
  13. Bencosme, S.A., Frei, J.: Relation of glucagon to A-cells of the pancreas. Proc. Soc. exp. Biol. (N.Y.) 91, 589–592(1956).Google Scholar
  14. Bencosme, S.A., Liepa, E., Lazarus, S.: Glucagon content of pancreatic tissue devoid of alpha cells. Proc. Soc. exp. Biol. (N.Y.) 90, 387–392(1955).Google Scholar
  15. Bencosme, S.A., Pease, D.C.: Electron microscopy of the pancreatic islets. Endocrinology 63, 1–13(1958).PubMedGoogle Scholar
  16. Bergen, S.S., jr., Itallie, T.B. van: Glucagon. An interim report. Metabolism 9, 132–156(1960).PubMedGoogle Scholar
  17. Berthet, J.: Some aspects of the glucagon problem. Amer. J. Med. 26, 703–714(1959).PubMedGoogle Scholar
  18. Bilbrey, G.L., Faloona, G.R., White, M.G., Knockel, J.P.: Hyperglucagonemia of renal failure. J. clin. Invest. 53, 841–847(1974).PubMedGoogle Scholar
  19. Björkman, N., Hellerström, C., Hellman, B., Petersson, B.: The cell types in the endocrine pancreas of the human fetus. Z. Zellforsch. 72, 425–445(1966).PubMedGoogle Scholar
  20. Björkman, N., Hellman, B.: Ultrastructure of the pancreatic A2-cells in different species. In: Ultrastructure and metabolism of the pancreatic islets, S.E. Brolin, B. Hellman K. Knutson, eds., p. 131–142. New York: Pergamon Press 1964.Google Scholar
  21. Blackard, W. G., Andrews, S.S., Lazarus, E.J.: Effect of growth hormone deficiency on glucagon secretion. Proc. Soc. exp. Biol. (N.Y.) 143, 1042–1044(1973).Google Scholar
  22. Blazquez, E., Sugase, T., Blazquez, M., Foa, P. P.: The ontogeny of metabolic regulation in the rat with special reference to the development of insular function. Acta diabet. lat. 9, Suppl. 1, 13–35 (1972).Google Scholar
  23. Bloom, S.R., Daniel, P.M., Johnston, D.I., Ogawa, O., Pratt, O. E.: Release of glucagon induced by stress. Quart. J. exp. Physiol. 58, 99–108(1973).PubMedGoogle Scholar
  24. Bloom, S.R., Johnston, D.I.: Failure of glucagon release of infants of diabetic mothers. Brit. med. J. 1972 IV, 453–454.Google Scholar
  25. Böttger, I., Dobbs, R., Faloona, G.R., Unger, R.H.: The effects of triglyceride absorption upon glucagon, insulin and gut glucagon-like immunoreactivity. J. clin. Invest. 52, 2532–2541(1973).PubMedGoogle Scholar
  26. Böttger, I., Faloona, G.R., Unger, R.H.: The effect of calcium and other salts upon the release of glucagon-like immunoreactivity (GLI) from the gut. J. clin. Invest. 51, 831–836(1972a).Google Scholar
  27. Böttger, I., Schlein, E.M., Faloona, G.R., Knockel, J. P., Unger, R.H.: The effect of exercise on glucagon secretion. J. clin. Endocr. 35, 117–125(1927b).Google Scholar
  28. Bromer, W. W., Behrens, O.K.: Glucagon. In: Methods in hormone research, R.I. Dorfman, ed., tome II, p. 459–475. New York-London: Academic Press 1962.Google Scholar
  29. Bromer, W. W., Boucher, M.E., Koffenberger, J.E. jr.: Amino acid sequence of bovine glucagon. J. biol. Chem. 246, 2822–2827(1971).PubMedGoogle Scholar
  30. Bromer, W. W., Sinn, L.G., Staub, A., Behrens, O.K.: The amino acid sequence of glucagon. Diabetes 6, 234–238(1957).PubMedGoogle Scholar
  31. Buchanan, K.D., Vance, J. E., Aoki, T., Williams, R.H.: Rise in serum immunoreactive glucagon after intrajejunal glucose in pancreatectomized dogs. Proc. Soc. Exp. Biol. (N.Y.) 126, 813–815(1967).Google Scholar
  32. Buchanan, K.D., Vance, J.E., Dinstl, K., Williams, R.H.: Effect of blood glucose on glucagon secretion in anesthetized dogs. Diabetes 18, 11–18(1969a).Google Scholar
  33. Buchanan, K.D., Vance, J.E., Morgan, A., Williams, R.H.: Effect of pancreozymin on insulin and glucagon levels in blood and bile. Amer. J. Physiol. 215, 1293–1298(1968).PubMedGoogle Scholar
  34. Buchanan, K.D., Vance, J.E., Wlliams, R.H.: Insulin and glucagon release from isolated islets of Langerhans. Effect of enteric factors. Diabetes 18, 381–386(1969b).Google Scholar
  35. Buckman, M.T., Conway, M.J., Seibel, J.A., Eaton, R.P.: Effect of fasting on alanine-stimulated insulin and glucagon secretion. Metabolism 22, 1253–1262(1973).PubMedGoogle Scholar
  36. Bürger, M., Brandt, W.: Über das Glukagon (die hyperglykamisierende Substanz des Pankreas). Z. ges. exp. Med. 96, 375–397(1935).Google Scholar
  37. Cavallero, C.: Etudes sur le facteur hyperglycemias du pancreas (glucagon). Rev. canad. Biol. 12, 509–529(1953).PubMedGoogle Scholar
  38. Chesney, T.M., Schofield, J. G.: Studies on the secretion of pancreatic glucagon. Diabetes 18, 627–632(1969).PubMedGoogle Scholar
  39. Crooks, R.J.M., Hulsmans, H.A.M., Israel, D.E., Hackeng, W.H.L., Schopman, W.: Glucagonoma as part of the polyglandular adenoma syndrome. Amer. J. Med. 52, 690–698(1972).Google Scholar
  40. Day, J.L., Knight, M., Condon, J. R.: The role of pancreatic glucagon in the pathogenesis of acute pancreatitis. Clin. Sci. 43, 597–603(1972).PubMedGoogle Scholar
  41. Dupre, J., Curtis, J. D., Unger, R.H., Waddell, R. W., Beck, J. C.: Effects of secretin, pancreozymin or gastrin on the response of the endocrine pancreas to administration of glucose or arginine in man. J. clin. Invest. 48, 745–757(1969).PubMedGoogle Scholar
  42. Duve, C.de: Glucagon, the hyperglycaemic glycogenolytic factor of the pancreas. Lancet 1953 II, 99–104.Google Scholar
  43. Edwards, J. C., Howell, S.L., Taylor, K. W.: Fatty acids as regulators of glucagon secretion. Nature (Lond.) 224, 808–809(1969).Google Scholar
  44. Edwards, J. C., Taylor, K. W.: Fatty acids and the release of glucagon from isolated guinea-pig islets of Langerhans incubated in vitro. Biochim. biophys. Acta (Amst.) 215, 310–315(1970).Google Scholar
  45. Eisentraut, A.M., Whissen, N., Unger, R.H.: Incubation damage in the radioimmunoassay for human plasma glucagon and its prevention with “Trasylol”. Amer. J. med. Sci. 225, 137–142(1968).Google Scholar
  46. Exton, J. E., Park, C.R.: The stimulation of gluconeogenesis from lactate by epinephrine, glucagon, and cyclic 3′,5′-adenylate in the perfused rat liver. Pharmacol. Rev. 18, 181–188(1966).PubMedGoogle Scholar
  47. Faller, A.: Elektronenmikroskopische Differenzierung verschiedener Inselzelltypen im Pankreas normaler Albinoratten. Z. Zellforsch. 97, 226–248(1969).PubMedGoogle Scholar
  48. Farmer, R. W., Pelizzari, E.D., Fabre, L.F., jr., Nonaka, K., Sugase, T., Foa, P. P.: Failure of growth hormone to stimulate glucagon secretion. Proc. Soc. exp. Biol. (N.Y.) 138, 491–493(1971).Google Scholar
  49. Felig, P., Pozefsky, T., Marliss, E., Cahill, G.F., jr.: Alanine: key role in gluconeogenesis. Science 167, 1003–1004(1970).PubMedGoogle Scholar
  50. Felig, P., Wahren, J., Hendler, R., Ahlborg, G.: Plasma glucagon levels in exercising man. New. Engl. J. Med. 287, 184–185(1972).PubMedGoogle Scholar
  51. Foa, P.P., Galansino, G., Pozza, G.: Glucagon, a second pancreatic hormone. Recent Progr. Hormone Res. 13, 473–510(1957).PubMedGoogle Scholar
  52. Fussgänger, R.D., Straub, K., Goberna, R., Jaros, P., Schröder, K.E., Raptis, S., Pfeiffer, E.F.: Primary secretion of insulin and secondary release of glucagon from the isolated perfused rat pancreas following stimulation with pancreozymin. Horm. Metab. Res. 1, 224–227(1969).PubMedGoogle Scholar
  53. Gaede, K., Ferner, H., Kastrup, H.: Über das zweite Kohlenhydratstoffwechselhormon der Bauch speicheldrüse (Glucagon) und seine Herkunft aus dem a-Zellensystem. Klin. Wschr. 28, 388–393(1950).PubMedGoogle Scholar
  54. Galloway, J. A.: The pharmacology and clinical use of glucagon. In: Glucagon, molecular physiology, clinical and therapeutic implication, P.J. Lefebvre and R.H. Unger, eds., p. 299–318. Oxford: Pergamon Press 1972.Google Scholar
  55. Gerich, J.E., Karam, J.H., Forscham, P.H.: Stimulation of glucagon secretion by epinephrine in man. J. clin. Endocr. 37, 479–480(1973a).Google Scholar
  56. Gerich, J.E., Langlois, M., Noacco, C.: Glucagon secretion in obesity. Lancet 1973 I, 1323.Google Scholar
  57. Gerich, J.E., Langlois, M., Noacco, C., Karam, J.H., Forsham, P.H.: Lack of glucagon response to hypoglycemia in diabetes: evidence for an intrinsic pancreatic alpha cell defect. Science 182, 171–173(1973c).Google Scholar
  58. Gerich, J.E., Langlois, M., Schneider, V., Karam, J.H., Noacco, C.: Effects of alteration of plasma free fatty acid levels on pancreatic glucagon secretion in man. J. clin. Invest. 53,1284–1289(1974a).Google Scholar
  59. Gerich, J.E., Schneider, V., Dippe, S.E., Langlois, M., Noacco, C., Karam, J.H., Forsham, P.H.: Characterization of the glucagon response to hypoglycemia in man. J. clin. Endocr. 38, 77–82(1974b).Google Scholar
  60. Girard, J., Bal, D., Assan, R.: Glucagon secretion during the early postnatal period in the rat. Horm. metab. Res. 4, 168–170(1972).PubMedGoogle Scholar
  61. Goldfine, I.D., Kirsteins, L., Lawrence, A.M.: Excessive glucagon responses to arginine in active acromegaly. Horm. metab. Res. 4, 97–100(1972).PubMedGoogle Scholar
  62. Gomez-Acebo, J., Parrilla, R., Candela, J.L.R.: Fine structure of the A and D cells of the rabbit endocrine pancreas in vivo and incubated in vitro. I. Mechanism of secretion of the A cells. J. Cell Biol. 36, 33–44(1968).PubMedGoogle Scholar
  63. Goodman, D.B.P., Rasmussen, H., Dibella, F., Guthrow, C.E.: Cyclic adenosine 3′ 4′-monophosphate stimulated phosphorylation of isolated neurotubule subunits. Proc. nat. Acad. Sci. (Wash.) 67, 652–659(1970).Google Scholar
  64. Grande, F.: Effect of glucagon on plasma free fatty acids and blood sugar in birds. Proc. Soc. exp. Biol. (N.Y.) 128, 532–536(1968).Google Scholar
  65. Grenier, J.F., Gillet, M., Kachelhoffer, J., Wong, P., Arriaga, R., Moody, A.J., Heding, L.G., Markussen, J., Sundby, F.: Circulation extra-corporelle d’intestin isole chez le chien. Mise en evidence d’une secretion par l’intestin de type glucagon. C.R. Soc. Biol. (Paris) 163, 2446–2449(1969).Google Scholar
  66. Grey, N., Mcguigan, J., Kipnis, D.: Neutralization of endogenous glucagon by high titer glucagon antiserum. Endocrinology 86, 1383–1388(1970).PubMedGoogle Scholar
  67. Grodsky, G.M., Hayashida, T., Peng, C.T., Geschwind, I.I.: Production of glucagon antibodies and their role in metabolism and immunoassay of glucagon. Proc. Soc. exp. Biol. (N.Y.) 107, 491–494(1961).Google Scholar
  68. Grollman, A., Mccaleb, W.E., White, F.N.: Glucagon deficiency as a cause of hypoglycemia. Metabolism 13, 686–690(1964).PubMedGoogle Scholar
  69. Guder, W., Frolich, J., Patzelt, C., Wieland, O.: The effect of glucagon on the state of lysosomal enzymes in isolated perfused rat liver. FEBS Letters 10, 215–218(1970).PubMedGoogle Scholar
  70. Gutman, R.A., Fink, G., Voyles, N., Selawry, H., Penhos, J. C., Lepp, A., Recant, L.: Specific biologic effects of intestinal glucagon-like materials. J. clin. Invest. 52, 1165–1175(1973).PubMedGoogle Scholar
  71. Hazzard, W.R., Crockford, P.M., Buchanan, K.D., Vance, J.E., Chen, R., Williams, R.H.: A double antibody immunoassay for glucagon. Diabetes 17, 179–186(1968).PubMedGoogle Scholar
  72. Heding, L.G.: The production of glucagon antibodies in rabbits. Horm. Metab. Res. 1, 87–88(1969).PubMedGoogle Scholar
  73. Heding, L.G.: Radioimmunological determination of pancreatic and gut glucagon in plasma. Diabetologia 7, 10–19(1971).PubMedGoogle Scholar
  74. Heding, L.G.: Immunologic properties of pancreatic glucagon: antigenicity and antibody characteristics. In: Glucagon, molecular physiology, clinical and therapeutic implications, P.J. Lefebvre and R.H. Unger, eds., p. 187–200. Oxford: Pergamon Press 1972.Google Scholar
  75. Heding, L.G., Rasmussen, S.M.: Determination of pancreatic and gut glucagon-like immunoreactivity (GLI) in normal and diabetic subjects. Diabetologia 8, 408–411(1972).PubMedGoogle Scholar
  76. Hellerström, C., Hellman, B., Petersson, P., Alm, G.: The two types of pancreatic A-cells and their relation to glucagon secretion. In: The structure and metabolism of the pancreatic islets, S.E. Brolin, B. Hellman and H. Knutson, eds., p. 117–130. New York: Pergamon Press 1964.Google Scholar
  77. Hellerström, C., Howell, S.L., Edwards, J. C., Andersson, A.: An investigation of glucagon biosynthesis in isolated pancreatic islets of guinea-pigs. FEBS Letters 27, 97–101(1972).PubMedGoogle Scholar
  78. Iversen, J.: Secretion of glucagon from the isolated, perfused canine pancreas. J. clin. Invest. 50, 2123–2136(1971).PubMedGoogle Scholar
  79. Iversen, J.: Adrenergic receptors and the secretion of glucagon and insulin from the isolated, perfused canine pancreas. J. clin. Invest. 52, 2102–2116(1973a).Google Scholar
  80. Iversen, J.: Effect of acetylcholine on the secretion of glucagon and insulin from the isolated perfused canine pancreas. Diabetes 22, 381–387(1973b).Google Scholar
  81. Jarousse, C., Rancon, F., Rosselin, G.: Hormonogenese perinatale de l’insuline et du glucagon chez le rat. C.R. Acad. Sci. (Paris) 276, 585–588(1973).Google Scholar
  82. Kalkhoff, R. K., Gossain, V. V., Matute, M.L.: Plasma glucagon in obesity. Response to arginine glucose and protein administration. New Engl. J. Med. 289, 465–467(1973).PubMedGoogle Scholar
  83. Kaneto, A., Mizuno, Y., Tasaka, Y., Kosaka, K.: Stimulation of glucagon secretion by tetragastrin. Endocrinology 86, 1175–1180(1970).PubMedGoogle Scholar
  84. Kenny, A.J., Say, R.R.: Glucagon-like activity extractable from the gastro-intestinal tract of man and other animals. J. Endocr. 25, 1–7(1962).PubMedGoogle Scholar
  85. Kern, H.F.: The fine structure of pancreatic a-cells under normal and experimental conditions. In: The structure and metabolism of the pancreatic islets, S. Falkmer, B. Hellman and L.B. Täljedal, eds., p. 99–107. Oxford: Pergamon Press 1970.Google Scholar
  86. Kimball, C.P., Murlin, J. R.: Aqueous extracts of pancreas. III. Some precipitation reactions of insulin. J. biol. Chem. 58, 337–346(1923).Google Scholar
  87. Krug, E., Mialhe, P.: Pancreatic and intestinal glucagon in the duck. Horm. Metab. Res. 3, 24–27(1971).PubMedGoogle Scholar
  88. Kuzuya, T., Kajinuma, H., Ide, T.: Effect of intrapancreatic injection of potassium and calcium on insulin and glucagon secretion in dogs. Diabetes 23, 55–60(1974).PubMedGoogle Scholar
  89. Lacy, P.E.: Electron microscopy of the normal islets of Langerhans. Studies in the dog, rabbit, guinea-pig and rat. Diabetes 6, 498–507(1957).PubMedGoogle Scholar
  90. Lange, R.: Immunofluoreszenzmikroskopische Darstellung glukagon-bildender Zellen an Plastikdimnschnitten von Inselgewebe (Ratte, Frosch). Histochemie 22, 226–233(1970).PubMedGoogle Scholar
  91. Langslow, D.R., Hales, C.N.: Bioassay of glucagon in human serum. Lancet 1970 I, 1151–1152.Google Scholar
  92. Laube, H., Fussganger, R., Goberna, R., Schröder, K., Straub, K., Sussman, K., Pfeiffer, E.F.: Effects of tolbutamide on insulin and glucagon secretion of the isolated perfused rat pancreas. Horm. Metab. Res. 3, 238–242(1971).PubMedGoogle Scholar
  93. Lawrence, A.M.: Radioimmunoassayable glucagon levels in man: effects of starvation, hypoglycemia, and glucose administration. Proc. nat. Acad. Sci. (Wash.) 55, 316–320(1966).Google Scholar
  94. Lawrence, A.M.: Glucagon. Ann. Rev. Med. 20, 207–222(1969).PubMedGoogle Scholar
  95. Lawrence, A.M.: Pancreatic alpha-cell function in miscellaneous clinical disorders. In: Glucagon, molecular physiology, clinical and therapeutic implications, P.J. Lefebvre and R.H. Unger, eds., p. 259–274. Oxford: Pergamon Press 1972.Google Scholar
  96. Lazarow, A.: Cell types of the islets of Langerhans and the hormones they produce. Diabetes 6, 222–233(1957).PubMedGoogle Scholar
  97. Lazarus, S.S., Shapiro, S., Volk, B.W.: Secretory granule formation and release in rabbit pancreatic A-cells. Diabetes 17, 152–160(1968).PubMedGoogle Scholar
  98. Leclercq-Meyer, V., Brisson, G.R., Malaisse, W. J.: Effect of epinephrine and glucose on glucagon and insulin release in vitro. Nature, (Lond.) New Biol. 231, 248–249(1971).Google Scholar
  99. Leclercq-Meyer, V., Marchand, J., Malaisse, W.J.: The effect of calcium and magnesium on glucagon secretion. Endocrinology 93, 1360–1370(1973).PubMedGoogle Scholar
  100. Leclercq-Meyer, V., Mialhe, P., Malaisse, W.J.: Une methode de dosage radioimmunologique du glucagon compartant une separation par le charbon-dextran. Diabetologia 6, 121–129(1970).PubMedGoogle Scholar
  101. Lefebvre, P.: The physiological effect of glucagon on fat mobilisation. Diabetologia 2, 130–132(1966).PubMedGoogle Scholar
  102. Lefebvre, P.: Glucagon and lipid metabolism. In: Glucagon, molecular physiology, clinical and therapeutic implications, P.J. Lefebvre and R.H. Unger, eds., p. 109–121. Oxford: Pergamon Press 1972.Google Scholar
  103. Lefebvre, P. J., Unger, R.H., Valverde, I., Rigopoulou, D., Luyckx, A.S., Eisentraut, A.: Effect of dog jejunum “glucagon-like immunoreactive material” on adipose tissue metabolism. Horm. Metab. Res. 1, 143–144(1969).PubMedGoogle Scholar
  104. Lightman, S.L., Bloom, S.R.: Cure of insulin-dependent diabetes mellitus by removal of a glucagonoma. Brit. med. J. 1974 I, 367–368.Google Scholar
  105. Like, A. A., Orci, L.: Embryogenesis of the human pancreatic islets: a light and electron microscopic study. Diabetes 21, 511–534(1972).PubMedGoogle Scholar
  106. Lindsey, A., Santeusiano, F., Braaten, J., Faloona, G.R., Unger, R.H.: Pancreatic alpha-cell function in trauma. J. Amer. med. Ass. 227, 757–761(1974).Google Scholar
  107. Lomsky, R., Langr, F., Vortel, V.: Site of glucagon in the islets of Langerhans of man as studied by the immunofluorescent technic. Sborn. lek. 11, 585–595(1968).Google Scholar
  108. Lomsky, R., Langr, F., Vortel, V.: Demonstration of glucagon in islet cell adenomas of the pancreas by immunofluorescent technic. Amer. J. clin. Path. 51, 245–250(1969).Google Scholar
  109. Luyckx, A.S.: Immunoassays for glucagon. In: Glucagon, molecular physiology, clinical and therapeutic implications, P.J. Lefebvre and R.H. Unger, eds., p. 285–298, Oxford: Pergamon Press, 1972.Google Scholar
  110. Luyckx, A.S., Lefebvre, P.J.: Arguments for a regulation of pancreatic glucagon secretion by circulating plasma free fatty acids. Proc. Soc. exp. Biol. (N.Y.) 133, 524–528(1970).Google Scholar
  111. Luyckx, A.S., Lefebvre, P.J.: Mechanisms involved in the exercise-induced increase in glucagon secretion in rats. Diabetes 23, 81–93(1974a).Google Scholar
  112. Luyckx, A., Lefebvre, P.: The role of energy substrates in controlling glucagon secretion. Experimental studies. In: Diabetes, Proc. 8th Congr. Int. Diab. Fed., Brussels 1973, p. 190–202. Amsterdam: Excerpta Medica, ICS n° 312, 1974b.Google Scholar
  113. Machino, M., Sakuma, H.: Electron microscopy of islet alpha cells of domestic fowl. Nature (Lond.) 214, 808–809(1967).Google Scholar
  114. Madison, L.L., Seyffert, W.A., jr., Unger, R.H., Barker, B.: Effect of plasma free fatty acids on plasma glucagon and serum insulin concentrations. Metabolism 17, 301–304(1968).PubMedGoogle Scholar
  115. Makman, M.H., Sutherland, E.W., jr.: Use of liver adenyl cyclase for assay of glucagon in human gastro-intestinal tract and pancreas. Endocrinology 75, 127–134(1964).PubMedGoogle Scholar
  116. Malaisse, W.J.: Hormonal and environmental modification of islet activity. In: Handbook of physiology, section 7: Endocrinology, vol. I: Endocrine pancreas, D. Steiner and N. Freinkel, eds., p. 237–260. Washington: American Physiological Society 1972.Google Scholar
  117. Malaisse, W., Malaisse-Lagae, F., King, S.: Effects of neutral red and imidazole upon insulin secretion. Diabetologia 4, 370–374(1968).PubMedGoogle Scholar
  118. Marco, J., Baroja, I.M., Diaz-Fierros, M., Villanueva, M.L., Valverde, I.: Relationship between insulin and gut glucagon-like immunoreactivity (GLI) secretion in normal and gastrectomized subjects. J. clin. Endocr. 34, 188–191(1972).PubMedGoogle Scholar
  119. Marco, J., Calle, C., Roman, D., Diaz-Fierros, M., Villanueva, M.L., Valverde, I.: Hyperglucagonism induced by glucocorticoid treatment in man. New Engl. J. Med. 288, 128–131 (1973a).Google Scholar
  120. Marco, J., Diego, J., Villanueva, M.L., Diaz-Fierros, M., Valverde, L, Segovia, J.M.: Elevated plasma glucagon levels in cirrhosis of the liver. New Engl. J. Med. 289, 1107–1111(1973b).Google Scholar
  121. Marco, J., Faloona, G.R., Unger, R.H.: The glycogenolytic activity of immunoreactive glucagon in plasma. J. clin. Invest. 50, 1650–1655(1971).PubMedGoogle Scholar
  122. Marco, J., Valverde, I.: Unaltered glucagon secretion after seven days of sulfonylurea administration in normal subjects. Diabetologia 9, Suppl., 317–319(1973).Google Scholar
  123. Markussen, J., Frandsen, E., Heding, L.G., Sundby, F.: Turkey glucagon: crystallization, amino acid composition and immunology. Horm. Metab. Res. 4, 360–363(1972).PubMedGoogle Scholar
  124. Marliss, E.B., Aoki, T.T., Cahill, G.F., jr.: Glucagon and amino-acid metabolism. In: Glucagon, molecular physiology, clinical and therapeutic implications, P.J. Lefebvre and R.H. Unger, eds., p. 123–150. Oxford: Pergamon Press 1972.Google Scholar
  125. Marliss, E.B., Aoki, T.T., Unger, R.H., Soeldner, J.S., Cahill, G.F., jr.: Glucagon levels and metabolic effects in fasting man. J. clin. Invest. 49, 2256–2270(1970).PubMedGoogle Scholar
  126. Marliss, E.B., Girard, L., Seydoux, J., Wollheim, C.B., Kanazawa, Y., Orci, L., Renold, A.E., Porte, D., jr.: Glucagon release induced by pancreatic stimulation in the dog. J. clin. Invest. 52, 1246–1259(1973a).Google Scholar
  127. Marliss, E.B., Wollheim, C.B., Blondel, B., Orci, L., Lambert, A.E., Stauffacher, W., Like, A., Renold, A.E.: Insulin and glucagon release from monolayer cell cultures of pancreas from newborn rats. Europ. J. clin. Invest. 3, 16–26(1973b).Google Scholar
  128. McGavran, M.H., Unger, R.H., Recant, L., Polk, H. C., Kilo, C., Levin, M.E.: A glucagonsecreting alpha-cell carcinoma of the pancreas. New Engl. J. Med. 274, 1408–1413(1966).PubMedGoogle Scholar
  129. McQuarrie, I., Bell, E.T., Zimmermann, B., Wright, W. S.: Deficiency of alpha cells of pancreas as possible ethiological factor in familial hypoglycemosis. Fed. Proc. 9, 337(1950).Google Scholar
  130. Mialhe, P.: Glucagon, insuline et regulation endocrine de la glycemie chez le canard. Acta endocr. (Kbh.), Suppl. 36, 1–134(1958).Google Scholar
  131. Miller, L.L.: Direct actions of insulin, glucagon and epinephrine on the isolated perfused rat liver. Fed. Proc. 24, 737–744(1965).PubMedGoogle Scholar
  132. Moody, A.J., Markussen, J., Schaich Fries, A., Steenstrup, C., Sundby, F., Malaisse, W., Malaisse-Lagae, F.: The insulin releasing activities of extracts of pork intestine. Diabetologia 6, 135–140(1970).PubMedGoogle Scholar
  133. Müller, W. A., Faloona, G.R., Aguilar-Parada, E., Unger, R.H.: Abnormal alpha-cell function in diabetes. Response to carbohydrate and protein ingestion. New Engl. J. Med. 283, 109–115(1970).PubMedGoogle Scholar
  134. Müller, W.A., Faloona, G.R., Unger, R.H.: The effect of alanine on glucagon secretion. J. clin. Invest. 50, 2215–2218(1971).PubMedGoogle Scholar
  135. Munger, B. L.: The secretory cycle of the pancreatic islet a-cell. An electron microscopic study of normal and synthalin-treated rabbits. Lab. Invest. 11, 885–901(1962).PubMedGoogle Scholar
  136. Noe, B.D., Bauer, G.E.: Evidence for glucagon biosynthesis involving a protein intermediate in islets of the Anglerfish (Lophius americanus). Endocrinology 89, 642–651(1971).PubMedGoogle Scholar
  137. Noe, B.D., Bauer, G.E.: Further characterization of a glucagon precursor from Anglerfish islet tissue. Proc. Soc. exp. Biol. (N.Y.) 142, 210–213(1973).Google Scholar
  138. Nonaka, K., Foa, P.P.: A simplified glucagon immunoassay and its use in a study of incubated pancreatic islets. Proc. Soc. exp. Biol. (N.Y.) 130, 330–336(1969).Google Scholar
  139. Ohneda, A., Aguillar-Parada, E., Eisentraut, A.M., Unger, R.H.: Control of pancreatic glucagon secretion by glucose. Diabetes 18, 1–10(1969).PubMedGoogle Scholar
  140. Ohneda, A., Parada, E., Eisentraut, A.M., Unger, R.H.: Characterization of response of circulating glucagon to intraduodenal and intravenous administration of amino acids. J. clin. Invest. 47, 2305–2322(1968).PubMedGoogle Scholar
  141. Okada, N., Takaki, R., Kitagawa, M.: Histologie and immunofluorescent studies on the site of origin of glucagon in mammalian pancreas. J. Histochem. Cytochem. 16, 405–409(1968).PubMedGoogle Scholar
  142. Orci, L., Lambert, A.E., Rouiller, C., Renold, A.E., Samols, E.: Evidence for the presence of A-cells in the endocrine fetal pancreas of the rat. Horm. Met. Res. 1, 108–110(1969).Google Scholar
  143. Orci, L., Pictet, R., Forssmann, W. G., Renold, A.E., Rouiller, C.: Structural evidence for glucagon producing cells in the intestinal mucosa of the rat. Diabetologia 4, 56–67(1968).PubMedGoogle Scholar
  144. Paloyan, E., Paloyan, D., Harper, P.V.: The role of glucagon hypersecretion in the relationship of pancreatitis and hyperparathyroidism. Surgery 62, 167–173(1967).Google Scholar
  145. Park, C.R., Exton, J. H.: Glucagon and the metabolism of glucose. In: Glucagon, molecular physiology, clinical and therapeutic implications, P. J. Lefebvre and R.H. Unger, eds. p. 77–108. Oxford: Pergamon Press 1972.Google Scholar
  146. Pearce, A.G.E., Polak, J.M., Heath, C.M.: Development, differentiation and derivation of the endocrine polypeptide cells of the mouse pancreas. Immunofluorescence, cytochemical and ultrastructural studies. Diabetologia 9, 120–129(1973).Google Scholar
  147. Pek, S., Fajans, S.S., Floyd, J. C., Jr., Knopf, R.F.: Clinical conditions with elevated plasma levels of glucagon. In: Diabetes, Proc. 8th Congr. Int. Diabetes Fed., Brussels 1973, p. 207–213. Amsterdam: Excerpta medica, ICS n° 312, 1974.Google Scholar
  148. Pek, S., Fajans, S.S., Floyd, J. C., jr., Knopf, F.R., Conn, J. W.: Failure of sulfonylureas to suppress plasma glucagon in man. Diabetes 21, 216–223(1972).PubMedGoogle Scholar
  149. Perrier, H., Billat, C.: Evolution de l’ulstrastructure du pancreas chez le foetus de rat. Diabetologia 6, 605–615(1970).PubMedGoogle Scholar
  150. Perrier, H., Porte, A., Jacquot, R.: Presence de cellules A dans le pancreas foetal de rat. C.R. Acad. Sci., serie D, 269, 841–843(1969).Google Scholar
  151. Persson, I., Gyntelberg, F., Heding, L.G., Boss-Nielsen, J.: Pancreatic glucagon-like immunoreactivity after intravenous insulin in normal and chronic-pancreatitis patients. Acta endocr. (Kbh.) 67, 401–404(1971).Google Scholar
  152. Petersson, B.: The two types of alpha cells during the development of the guinea-pig pancreas. Z. Zellforsch. 75, 371–380(1966).PubMedGoogle Scholar
  153. Petersson, B., Hellman, B.: Effects of long term administration of glucagon on the pancreatic islet tissue of rats and guinea-pigs. Acta endocr. (Kbh.) 44, 139–149(1963).Google Scholar
  154. Pictet, R., Rutter, W. J.: Development of the embryonic endocrine pancreas. In: Handbook of physiology, section 7: Endocrinology, vol. I: Endocrine pancreas, D.F. Steiner and N. Freinkel, eds., p. 25–66. Washington: American Physiological Society 1972.Google Scholar
  155. Pincus, I.J., Rutman, J.Z.: Glucagon, the hyperglycemic agent in pancreatic extracts. Arch. intern. Med. 92, 666–677(1953).Google Scholar
  156. Rabinovitch, A., Dupre, J.: Effects of the gastric inhibitory polypeptide present in impure pancreozymin-cholecystokinin on plasma insulin and glucagon in the rat. Endocrinology 94, 1139–1144(1974).PubMedGoogle Scholar
  157. Rasmussen, H., Tenenhouse, A.: Cyclic adenosine monophosphate, Ca++, and membranes. Proc. nat. Acad. Sci. (Wash.) 59, 1364–1370(1968).Google Scholar
  158. Rehfeld, J.F., Heding, L.G.: Increased release of gut glucagon in reactive hypoglycaemia. Brit. med. J. 1970 II, 706–707.Google Scholar
  159. Rigopoulou, D., Valverde, I., Marco, J., Faloonga, G., Unger, R.H.: Large glucagon immunoreactivity in extracts of pancreas. J. biol. Chem. 245, 496–501(1970).PubMedGoogle Scholar
  160. Rocha, D.M., Faloona, G.R., Unger, R.H.: Glucagon-stimulating activity of 20 amino-acids in dogs. J. clin. Invest. 51, 2346–2351(1972).PubMedGoogle Scholar
  161. Rocha, D.M., Santeusiano, F., Faloona, G.R., Unger, R.H.: Abnormal pancreatic alpha-cell function in bacterial infections. New Engl. J. Med. 288, 700–703(1973).PubMedGoogle Scholar
  162. Rodbell, M.: Regulation of glucagon action at its receptors. In: Glucagon, molecular physiology, clinical and therapeutic implications, P.J. Lefebvre and R.H. Unger, eds., p. 61–75. Oxford: Pergamon Press 1972.Google Scholar
  163. Rodbell, M., Birnbaumer, L., Pohl, S.L., Michiel, H., Krans, J.: Properties of the adenyl cyclase systems in liver and adipose cells: the mode of action of hormones. In: On the pathogenesis of diabetes mellitus, R. Luft and P.J. Randle, eds., p. 9–57. Milano: Casa Editrice „Il ponte“ 1970.Google Scholar
  164. Samols, E., Marks, V.: Nouvelles conceptions sur la signification fonctionnelle du glucagon (pancreatique et extrapancreatique). In: Journees Annuelles de Diabetologie de l’Hotel Dieu, p. 43–66. Paris: Editions Medicales Flammarion 1967.Google Scholar
  165. Samols, E., Marri, G., Marks, V.: Promotion of insulin secretion by glucagon. Lancet 1965a II, 415–416.Google Scholar
  166. Samols, E., Tyler, J.M., Marks, V.: Glucagon-insulin relationships. In: Glucagon, molecular physiology, clinical and therapeutic implications, P.J. Lefebvre and R.H. Unger, eds., p. 151–173. Oxford: Pergamon Press 1972.Google Scholar
  167. Samols, E., Tyler, J., Marri, G., Marks, V.: Stimulation of glucagon secretion by oral glucose. Lancet 1965b II, 1257–1259.Google Scholar
  168. Samols, E., Tyler, J., Megyesi, C., Marks, V.: Immunochemical glucagon in human pancreas, gut, and plasma. Lancet 1966 II, 727–729.Google Scholar
  169. Samols, E., Tyler, J.M., Mialhe, P.: Suppression of pancreatic glucagon release by the hypoglycaemic sulfonylureas. Lancet 1969 I, 174–176.Google Scholar
  170. Santeusiano, F., Faloona, G.R., Knockel, J.P., Unger, R.H.: Evidence for a role of endogenous insulin and glucagon in the regulation of potassium homeostasis. J. Lab. clin. Med. 81, 809–817(1973a).Google Scholar
  171. Santeusiano, F., Faloona, G.R., Unger, R.H.: Suppressive effect of secretin upon pancreatic alpha cell function. J. clin. Invest. 51, 1743–1749(1972).Google Scholar
  172. Santeusiano, F., Faloona, G.R., Unger, R.H.: Inhibition of alanine-stimulated glucagon secretion by secretin in the dog. Horm. Metab. Res. 5, 425–427(1973b).Google Scholar
  173. Sato, T., Herman, L., Fitzgerald, P.J.: The comparative ultrastructure of the pancreatic islet of Langerhans. Gen. comp. Endocr. 7, 132–157(1966).Google Scholar
  174. Schopman, W., Hackeng, W.H.L., Steendijk, C.: The purification of 125I-glucagon of high specific activity for radioimmunochemical estimation of glucagon and a qualitative comparison of glucagon from different sources. Acta endocr. (Kbh.) 54, 527–540(1967).Google Scholar
  175. Shima, K., Foa, P.P.: A double antibody assay for glucagon. Clin. chim. Acta 22, 511–520(1968).PubMedGoogle Scholar
  176. Sokal, J.E.: Effect of glucagon on gluconeogenesis by the isolated perfused rat liver. Endocrinology 78, 538–548(1966a).Google Scholar
  177. Sokal, J.E.: Glucagon. An essential hormone. Amer. J. Med. 41, 331–341(1966b).Google Scholar
  178. Sokal, J.E.: Bioassays for glucagon. In: Glucagon, molecular physiology, clinical and therapeutic implications, P.J. Lefebvre and R.H. Unger, eds., p. 275–284. Oxford: Pergamon Press 1972.Google Scholar
  179. Sokal, J.E., Ezdinli, E.Z.: Basal plasma glucagon levels of man. J. clin. Invest. 46, 778–785(1967).PubMedGoogle Scholar
  180. Sokal, J.E., Sarcione, E.J., Henderson, A.M.: Relative potency of glucagon and epinephrine as hepatic glycogenolytic agents: studies with the isolated perfused rat liver. Endocrinology 74, 930–938(1964).PubMedGoogle Scholar
  181. Sperling, M.A., Lamater, P.V., de, Phelps, D., Fiser, R.H., Oh, W., Fisher, D.A.: Spontaneous and amino acid-stimulated glucagon secretion in the immediate postnatal period. Relation to glucose and insulin. J. clin. Invest. 53, 1159–1166(1974).PubMedGoogle Scholar
  182. Staub, A., Sinn, L., Behrens, O. K.: Purification and crystallization of glucagon. J. biol. Chem. 214, 619–632(1955).PubMedGoogle Scholar
  183. Struck, E., Ashmore, J., Wieland, O.: Stimulierung der Gluconeogenese durch langkettige Fettsäuren und Glucagon. Biochem. Z. 343, 107–110(1965).PubMedGoogle Scholar
  184. Sundby, F., Markussen, J.: Isolation crystallization and amino acid composition of rat glucagon. Horm. Metab. Res. 3, 184–187(1971).PubMedGoogle Scholar
  185. Sundby, F., Markussen, J.: Rabbit glucagon: isolation crystallization and amino acid composition. Horm. Metab. Res. 4, 56(1972).PubMedGoogle Scholar
  186. Sutherland, E.W., Cori, C.F., Haynes, R., Olsen, N.S.: Purification of the hyperglycemicglycogenolytic factor from insulin and from gastric mucisa. J. biol. Chem. 180, 825–837 (1949).PubMedGoogle Scholar
  187. Sutherland, E.W., Duve, C. de: Origin and distribution of the hyperglycemic-glycogenolytic factor of the pancreas. J. biol. Chem. 175, 663–674(1948).PubMedGoogle Scholar
  188. Sutherland, E.W., Hardman, J.G., Butcher, R.W., Broadus, A.E.: The biological role of cyclic AMP (some areas of contrast with cyclic GMP). In: Progress in endocrinology, C. Gual, ed., p. 26–32. Amsterdam: Excerpta Medica Foundation, ICS n° 184, 1968.Google Scholar
  189. Tager, H.S., Steiner, D.F.: Isolation of a glucagon-containing peptide: primary structure of a possible fragment of proglucagon. Proc. nat. Acad. Sci. (Wash.) 70, 2321–2325(1973).Google Scholar
  190. Thomsen, J., Kristiansen, K., Brunfeldt, K., Sundby, F.: The amino acid sequence of human glucagon. FEBS Letters 21, 315–319(1972).PubMedGoogle Scholar
  191. Tung, A. K.: Biosynthesis of avian glucagon: evidence for a possible high molecular weight biosynthetic intermediate. Horm. Metab. Res. 5, 416–424(1973).PubMedGoogle Scholar
  192. Tung, A. K., Zerega, F.: Biosynthesis of glucagon in isolated pigeon islets. Biochem. biophys. Res. Commun. 45, 387–395(1971).PubMedGoogle Scholar
  193. Unger, R.H.: Glucagon and the insulin: glucagon ratio in diabetes and other catabolic illnesses. Diabetes 20, 834–838(1971).PubMedGoogle Scholar
  194. Unger, R.H.: Glucagon and glucagon immunoreactivity in plasma and pancreatic tissues. In: Glucagon, molecular physiology, clinical and therapeutic implications, P.J. Lefebvre and R.H. Unger, eds. p. 205–211. Oxford: Pergamon Press 1972a.Google Scholar
  195. Unger, R.H.: Pancreatic alpha-cell function in diabetes mellitus. In: Glucagon, molecular physiology, clinical and therapeutic implications, P.J. Lefebvre and R.H. Unger, eds., p. 245–257. Oxford: Pergamon Press 1972b.Google Scholar
  196. Unger, R.H.: Radioimmunoassay of glucagon. Metabolism 22, 979–985(1973a).Google Scholar
  197. Unger, R.H.: Normal and abnormal alpha cell function. Ann. Rev. Med. 24, 303–308(1973b).Google Scholar
  198. Unger, R.H., Aguilar-Parada, E., Müller, W.A., Eisentraut, A.M.: Studies of pancreatic alpha cell function in normal and diabetic subjects. J. clin. Invest. 49, 837–848(1970).PubMedGoogle Scholar
  199. Unger, R.H., Eisentraut, A.M.: Glucagon. In: Hormones in blood, C.H. Gray and A.L. Bucharach, eds., 2nd ed., vol. I, p. 83–128. London-New York: Academic Press 1967.Google Scholar
  200. Unger, R.H., Eisentraut, A.M.: Entero-insular axis. Arch. intern. Med. 123, 261–266(1969).PubMedGoogle Scholar
  201. Unger, R.H., Eisentraut, A.M., McCall, M.S., Keller, S., Lanz, H.C., Madison, L.L.: Glucagon antibodies and their use for immunoassay for glucagon. Proc. Soc. exp. Biol. (N.Y.) 102, 621–623(1959).Google Scholar
  202. Unger, R.H., Eisentraut, A.M., McCall, M.S., Madison, L.L.: Glucagon antibodies and an immunoassay for glucagon. J. clin. Invest. 40, 1280–1289(1961).PubMedGoogle Scholar
  203. Unger, R.H., Ketterer, H., Dupre, J., Eisentraut, A.M.: The effects of secretin, pancreozymin, and gastrin on insulin and glucagon secretion in anesthetized dogs. J. clin. Invest. 46, 630–645(1967).PubMedGoogle Scholar
  204. Unger, R.H., Ketterer, H., Eisentraut, A.M.: Distribution of immunoassayable glucagon in gastrointestinal tissues. Metabolism 15, 865–867(1966).PubMedGoogle Scholar
  205. Unger, R.H., Lefebvre, P.J.: Glucagon physiology. In: Glucagon, molecular physiology, clinical and therapeutic implications, P.J. Lefebvre and R.H. Unger, eds., p. 213–244. Oxford: Pergamon Press 1972.Google Scholar
  206. Unger, R.H., Lochner, J.V. de., Eisentraut, A.M.: Identification of insulin and glucagon in a bronchogenic metastasis. J. clin. Endocr. 24, 823–831(1964).PubMedGoogle Scholar
  207. Unger, R.H., Madison, L.L., Müller, W. A.: Abnormal alpha cell function in diabetics: response to insulin. Diabetes 21, 301–307(1972).Google Scholar
  208. Unger, R.H., Ohneda, A., Aguilar-Parada, E., Eisentraut, A.M.: The role of aminogenic glucagon secretion in blood glucose homeostasis. J. clin. Invest. 48, 810–822(1969).PubMedGoogle Scholar
  209. Unger, R.H., Ohneda, A., Valverde, I., Eisentraut, A.M., Exton, J.: Characterization of the responses of circulating glucagon like immunoreactivity to intraduodenal and intravenous administration of glucose. J. clin. Invest. 47, 48–65(1968).PubMedGoogle Scholar
  210. Valverde, I., Rigopoulou, D., Exton, J., Ohneda, A., Eisentraut, A.M., Unger, R.H.: Demonstration and characterization of a second fraction of glucagon-like immunoreactivity in jejunal extracts. Amer. J. med. Sci. 255, 415–420(1968).PubMedGoogle Scholar
  211. Valverde, I., Rigopoulou, D., Marco, J., Faloona, G.R., Unger, R.H.: Characterization of glucagon-like immunoreactivity (GLI). Diabetes 19, 614–623(1970).PubMedGoogle Scholar
  212. Vance, J.E., Buchanan, K.D., Challoner, D.R., Williams, R.H.: Effect of glucose concentration on insulin and glucagon release from isolated islets of Langerhans of the rat. Diabetes 17, 187–193(1968).PubMedGoogle Scholar
  213. Vance, J.E., Buchanan, K.D., Williams, R.H.: Glucagon and insulin release. Influence of drugs affecting the autonomic nervous system. Diabetes 20, 78–82(1971).PubMedGoogle Scholar
  214. Vance, J.E., Stoll, R. W., Kitabchi, A.E., Williams, R.H., Wood, F. C., jr.: Nesidioblastosis in familial endocrine adenomatosis. J. Amer. med. Ass. 207, 1679–1682(1969).Google Scholar
  215. Walsh, D.A., Krebs, E.G., Reimann, E.M., Brostrom, M.A., Corbin, J.D., Hickenbottom, J.P., Soderling, T.R., Perkins, J.P.: The receptor protein for cyclic AMP in the control of glycogenos is. In: Role of cyclic AMP in cell function, P. Greengard and E. Costa, eds., p. 265–285. New York: Raven Press 1970.Google Scholar
  216. Weinges, K. F.: Glucagon. In: Monographic in der Reihe Biochemie und Klinik, Hrsg. G. Weitzel und N. Zöllner. Stuttgart: Georg Thieme Verlag 1968.Google Scholar
  217. Weintraub, B., Sarcione, E.J., Sokal, J.E.: Effect of glucagon on phosphorylase activity of the isolated perfused rat liver. Amer. J. Physiol. 216, 521–526(1969).PubMedGoogle Scholar
  218. Wellman, K. F., Volk, B.W., Brancato, P.: Ultrastructure and insulin content of the endocrine pancreas in the human fetus. Lab. Invest. 25, 97–103(1971).Google Scholar
  219. Williamson, J.R., Wright, P.H., Malaisse, W. J., Ashmore, J.: Control of glucogenesis by acetyl CoA in rats treated with glucagon and anti-insulin serum. Biochem. biophys. Res. Commun. 24, 765–770(1966).Google Scholar
  220. Wilmore, D., Moylan, J.A., Pruitt, B.A., Lindsey, C.A., Faloona, G.R., Unger, R.H.: Hyperglucagonemia after burns. Lancet 1974 I, 73–74.Google Scholar
  221. Winborn, W. B.: Light and electron microscopy of the islets of Langerhans of the Saimiri Monkey pancreas. Anat. Rec. 147, 65–93(1963).PubMedGoogle Scholar
  222. Wise, J.K., Hendler, R., Felig, P.: Obesity: evidence for decreased secretion of glucagon. Science 178, 513–514(1972).PubMedGoogle Scholar
  223. Wise, J.K., Hendler, R., Felig, P.: Evaluation of alpha-cell function by infusion of alanine in normal, diabetic and obese subjects. New Engl. J. Med. 288, 484–487(1973a).Google Scholar
  224. Wise, J.K., Hendler, R., Felig, P.: Influence of glucocorticoids on glucagon secretion and plasma amino acid concentrations in man. J. clin. Invest. 52, 2774–2782(1973b).Google Scholar
  225. Worobec, R., Locke, R., Hall, A., Ertl, R., Ernst, K., Deininger, E.: Production of antibodies of high binding affinities to glucagon in rabbits. Biochem. biophys. Res. Commun. 29, 406–412(1967).PubMedGoogle Scholar
  226. Wunsch, E.: Die Totalsynthese des Pankreas-Hormons Glucagon. Z. Naturforsch. 22b, 1269–1276(1967).Google Scholar
  227. Wunsch, E., Weinges, K. F.: The synthesis of glucagon. Properties of synthetic glucagon. In: Glucagon, molecular physiology, clinical and therapeutic implications, P.J. Lefebvre and R.H. Unger, eds., p. 31–46. Oxford: Pergamon Press 1972.Google Scholar
  228. Wulf, H., de: The control of glycogen metabolism in the liver. Louvain: Vander 1971.Google Scholar
  229. Yoshinaga, T., Okuno, G., Shinji, Y., Tsujii, T., Nishikawa, M.: Pancreatic A-cell tumor associated with severe diabetes mellitus. Diabetes 15, 709–713(1966).PubMedGoogle Scholar

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© Springer-Verlag Berlin · Heidelberg 1975

Authors and Affiliations

  • Viviane Leclercq-Meyer
  • Willy J. Malaisse

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