Care and Handling of Sea Urchin Eggs, Embryos, and Adults (Principally North American Species)

  • R. Hinegardner

Abstract

Sea urchins are found along almost all coastlines, usually on shallow, rocky bottom, although some species live in deep water or in sand or silt (Table 2A.1). Seen from above, sea urchins are round or somewhat heart-shaped; from the side, they are more or less flat. Scutillidae — here referred to as sand dollars — are very flat. All sea urchins have spines: very long and sometimes poisonous as on the Centveohinidae, short and blunt as on the limpet-like Colobooentrotus, or very small and numerous as on the sand dollar. Sexes are separate — except for rare hermaphrodites — but can seldom be distinguished by external characteristics. Exceptions are Lytechinus and Psammechinus, the male and female of which have slightly different gonopores.

Keywords

Urea Cysteine Trypsin Dolomite Silt 

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References

  1. ANDERSON, E., 1968. Oocyte differentiation in the sea urchin Arbacia punctulata, with particular reference to the origin of cortical granules and their participation in the cortical reaction. J. Cell. Biol., 37, 514–539.PubMedCrossRefGoogle Scholar
  2. BERG, W.E., 1967. Some experimental techniques for eggs and embryos of marine invertebrates. In: Methods in Developmental Biology (F.H. Wilt and N.K. Wessells, eds.), p. 716–767. New York: Thomas Y. Crowell Co.Google Scholar
  3. BOOLOOTIAN, R.A., 1966. Reproductive Physiology. In: Physiology of Echinodermata (R.A. Boolootian, ed.), p. 561–613. New York: Interscience Publishers.Google Scholar
  4. BURCHILL, B.R., BLOMQUIST, C.H., 1969. Removal of fertilization membranes from sea urchin (Lytechinus pictus) eggs. Experientia, 25, 540–541.PubMedCrossRefGoogle Scholar
  5. CAMERON, R.A., HINEGARDNER, R.T., 1974. Initiation of metamorphosis in laboratory cultured sea urchins. Biol. Bull. 146, 335–342.PubMedCrossRefGoogle Scholar
  6. CAVANAUGH, G.M., 1956. Formulae and methods V. of the Marine Biological Laboratory Chemical Room, Woods Hole, Mass.: Marine Biological Laboratory.Google Scholar
  7. CITKOWITZ, E., 1971. The hyaline layer: its isolation and role in echinoderm development. Dev. Biol. 24.Google Scholar
  8. COSTELLO, D.P., DAVIDSON, M.E., EGGERS, A., FOX, M.H., HENLEY, C., 1957. Methods for obtaining and handling marine eggs and embryos. Woods Hole, Mass.: Marine Biological Laboratory.Google Scholar
  9. CZIHAK, G., 1960. Untersuchungen über die Coelomanlagen und die Metamorphose des Pluteus von Psammechinus miliaris. Zool. Jahrb. Abt. Anat. Ontog. Tiere 78, 235–256.Google Scholar
  10. ENDO, Y., 1961. Changes in the cortical layer of sea urchin eggs at fertilization as studied with the electron microscope. I Clypeaster japanicus. Exp. Cell Res., 25, 383–397.PubMedCrossRefGoogle Scholar
  11. EPEL, D., 1970. Methods for removal of the vitelline membrane of sea urchin eggs. II. Controlled exposure to trypsin to eliminate post-fertilization clumping of embryos. Exp. Cell Res., 61, 69–70.PubMedCrossRefGoogle Scholar
  12. EPEL, D., WEAVER, A.M., MAZIA, D., 1970. Methods for removal of the vitelline membrane of sea urchin eggs. I. Use of Dithiothreitol (Cleland Reagent). Exp. Cell Res., 61, 64–68.PubMedCrossRefGoogle Scholar
  13. GUILLARD, R.R.I., RYTHER, J.H., 1963. Studies on marine planktonic diatomes. I. Cyclotella nana Hustedt and Detonula confervacea (Cleve) Gran. Can. J. Microbiol., 8, 229–239.CrossRefGoogle Scholar
  14. HAGSTRöM, B.E., 1959. Further experiments on jelly-free sea urchin eggs. Exp. Cell Res., 17, 256.PubMedCrossRefGoogle Scholar
  15. HARVEY, E.B., 1956. The American Arbacia and other sea urchins. Princeton, N.J.: Princeton University Press.Google Scholar
  16. HINEGARDNER, R.T., 1967. Echinoderms. In: Methods in Developmental Biology (F.H. Wilt and N.K. Wessels, eds.), p. 139–155. New York: Thomas Y. Crowell Co.Google Scholar
  17. HINEGARDNER, R.T., 1969. Growth and development of the laboratory cultured sea urchin. Biol. Bull., 137, 465–475.PubMedCrossRefGoogle Scholar
  18. HöRSTADIUS, S., 1973. Experimental embryology of echinoderms. London: Oxford Univ. Press.Google Scholar
  19. HULTIN, T., 1948. Species specificity in fertilization reaction. I. The role of the vitelline membrane of sea urchin eggs in species specificity. Arkiu für Zoologi, 40A (12), 1–9.Google Scholar
  20. HYMEN, L.H., 1955. The Invertebrates: Echinodermata the coelomate bilateria, Vol. IV. New York: McGraw-Hill Book Co., Inc.Google Scholar
  21. KANE, R.E., 1970. Direct isolation of the hyaline layer protein released from the cortical granules of the sea urchin egg at fertilization. J. Cell Biol. 45, 615–622.PubMedCrossRefGoogle Scholar
  22. KARNOFSKY, D.A., Simmel, E.B., 1963. Effects of growth inhibiting chemicals on the sand dollar embryos Echinorachnius parma. Prog. Exp. Tumor Res. 3, 254–295.PubMedGoogle Scholar
  23. LINDAHL, P.E., LUNDIN, J., 1948. Removal of the fertilization membranes from large quantities of sea urchin eggs. Science, 108, 481–482.PubMedCrossRefGoogle Scholar
  24. MAZIA, D., MITCHISON, J.M., MEDINA, H., HARRIS, P., 1961. The direct isolation of the mitotic apparatus. J. Biophys. Biochem. Cytology, 10, 467.CrossRefGoogle Scholar
  25. MCBRIDE, E.W., 1903. The development of Echinus esculentus, together with some points in the development of E. miliaris and E. acutus. Phil. Trans. Roy. Soc. London, Series B, 195, 285–327.CrossRefGoogle Scholar
  26. MOORE, A.R., 1930. Fertilization and development without membrane formation in the egg of the sea urchin, Strongylocentrotus purpuratus. Protoplasma, 9, 9–17.CrossRefGoogle Scholar
  27. SHAPIRO, H., 1935. The validity of the centrifuge method for estimating aggregate cell volume in suspensions of the egg of the sea urchin, Arbacia punctulata. Biol. Bull., 68, 363–377.CrossRefGoogle Scholar
  28. SPOTTE, S.H., 1970. Fish and invertebrate culture, water management in closed systems. New York: Wiley-Interscience.Google Scholar
  29. TYLER, A., 1949. A simple, non-injurious method for inducing spawning of sea urchins and sand dollars. Collect. Net., 19, 19–20.Google Scholar
  30. TYLER, A., TYLER, B.S., 1966. The gametes; some procedure and properties. In: Physiology of Echinodermata (R.A. Boolootian, ed.), p. 639–682. New York: Interscience Publishers.Google Scholar
  31. VACQUIER, V.D., MAZIA, D., 1968. Twinning of sea urchin embryos by treatment with dithiothreitol. Exp. Cell Res., 52, 459–468.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1975

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  • R. Hinegardner

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