Skip to main content

Care and Handling of Sea Urchin Eggs, Embryos, and Adults (Principally North American Species)

  • Chapter

Abstract

Sea urchins are found along almost all coastlines, usually on shallow, rocky bottom, although some species live in deep water or in sand or silt (Table 2A.1). Seen from above, sea urchins are round or somewhat heart-shaped; from the side, they are more or less flat. Scutillidae — here referred to as sand dollars — are very flat. All sea urchins have spines: very long and sometimes poisonous as on the Centveohinidae, short and blunt as on the limpet-like Colobooentrotus, or very small and numerous as on the sand dollar. Sexes are separate — except for rare hermaphrodites — but can seldom be distinguished by external characteristics. Exceptions are Lytechinus and Psammechinus, the male and female of which have slightly different gonopores.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • ANDERSON, E., 1968. Oocyte differentiation in the sea urchin Arbacia punctulata, with particular reference to the origin of cortical granules and their participation in the cortical reaction. J. Cell. Biol., 37, 514–539.

    Article  PubMed  CAS  Google Scholar 

  • BERG, W.E., 1967. Some experimental techniques for eggs and embryos of marine invertebrates. In: Methods in Developmental Biology (F.H. Wilt and N.K. Wessells, eds.), p. 716–767. New York: Thomas Y. Crowell Co.

    Google Scholar 

  • BOOLOOTIAN, R.A., 1966. Reproductive Physiology. In: Physiology of Echinodermata (R.A. Boolootian, ed.), p. 561–613. New York: Interscience Publishers.

    Google Scholar 

  • BURCHILL, B.R., BLOMQUIST, C.H., 1969. Removal of fertilization membranes from sea urchin (Lytechinus pictus) eggs. Experientia, 25, 540–541.

    Article  PubMed  CAS  Google Scholar 

  • CAMERON, R.A., HINEGARDNER, R.T., 1974. Initiation of metamorphosis in laboratory cultured sea urchins. Biol. Bull. 146, 335–342.

    Article  PubMed  CAS  Google Scholar 

  • CAVANAUGH, G.M., 1956. Formulae and methods V. of the Marine Biological Laboratory Chemical Room, Woods Hole, Mass.: Marine Biological Laboratory.

    Google Scholar 

  • CITKOWITZ, E., 1971. The hyaline layer: its isolation and role in echinoderm development. Dev. Biol. 24.

    Google Scholar 

  • COSTELLO, D.P., DAVIDSON, M.E., EGGERS, A., FOX, M.H., HENLEY, C., 1957. Methods for obtaining and handling marine eggs and embryos. Woods Hole, Mass.: Marine Biological Laboratory.

    Google Scholar 

  • CZIHAK, G., 1960. Untersuchungen über die Coelomanlagen und die Metamorphose des Pluteus von Psammechinus miliaris. Zool. Jahrb. Abt. Anat. Ontog. Tiere 78, 235–256.

    Google Scholar 

  • ENDO, Y., 1961. Changes in the cortical layer of sea urchin eggs at fertilization as studied with the electron microscope. I Clypeaster japanicus. Exp. Cell Res., 25, 383–397.

    Article  PubMed  CAS  Google Scholar 

  • EPEL, D., 1970. Methods for removal of the vitelline membrane of sea urchin eggs. II. Controlled exposure to trypsin to eliminate post-fertilization clumping of embryos. Exp. Cell Res., 61, 69–70.

    Article  PubMed  CAS  Google Scholar 

  • EPEL, D., WEAVER, A.M., MAZIA, D., 1970. Methods for removal of the vitelline membrane of sea urchin eggs. I. Use of Dithiothreitol (Cleland Reagent). Exp. Cell Res., 61, 64–68.

    Article  PubMed  CAS  Google Scholar 

  • GUILLARD, R.R.I., RYTHER, J.H., 1963. Studies on marine planktonic diatomes. I. Cyclotella nana Hustedt and Detonula confervacea (Cleve) Gran. Can. J. Microbiol., 8, 229–239.

    Article  Google Scholar 

  • HAGSTRöM, B.E., 1959. Further experiments on jelly-free sea urchin eggs. Exp. Cell Res., 17, 256.

    Article  PubMed  Google Scholar 

  • HARVEY, E.B., 1956. The American Arbacia and other sea urchins. Princeton, N.J.: Princeton University Press.

    Google Scholar 

  • HINEGARDNER, R.T., 1967. Echinoderms. In: Methods in Developmental Biology (F.H. Wilt and N.K. Wessels, eds.), p. 139–155. New York: Thomas Y. Crowell Co.

    Google Scholar 

  • HINEGARDNER, R.T., 1969. Growth and development of the laboratory cultured sea urchin. Biol. Bull., 137, 465–475.

    Article  PubMed  CAS  Google Scholar 

  • HöRSTADIUS, S., 1973. Experimental embryology of echinoderms. London: Oxford Univ. Press.

    Google Scholar 

  • HULTIN, T., 1948. Species specificity in fertilization reaction. I. The role of the vitelline membrane of sea urchin eggs in species specificity. Arkiu für Zoologi, 40A (12), 1–9.

    Google Scholar 

  • HYMEN, L.H., 1955. The Invertebrates: Echinodermata the coelomate bilateria, Vol. IV. New York: McGraw-Hill Book Co., Inc.

    Google Scholar 

  • KANE, R.E., 1970. Direct isolation of the hyaline layer protein released from the cortical granules of the sea urchin egg at fertilization. J. Cell Biol. 45, 615–622.

    Article  PubMed  CAS  Google Scholar 

  • KARNOFSKY, D.A., Simmel, E.B., 1963. Effects of growth inhibiting chemicals on the sand dollar embryos Echinorachnius parma. Prog. Exp. Tumor Res. 3, 254–295.

    PubMed  CAS  Google Scholar 

  • LINDAHL, P.E., LUNDIN, J., 1948. Removal of the fertilization membranes from large quantities of sea urchin eggs. Science, 108, 481–482.

    Article  PubMed  CAS  Google Scholar 

  • MAZIA, D., MITCHISON, J.M., MEDINA, H., HARRIS, P., 1961. The direct isolation of the mitotic apparatus. J. Biophys. Biochem. Cytology, 10, 467.

    Article  CAS  Google Scholar 

  • MCBRIDE, E.W., 1903. The development of Echinus esculentus, together with some points in the development of E. miliaris and E. acutus. Phil. Trans. Roy. Soc. London, Series B, 195, 285–327.

    Article  Google Scholar 

  • MOORE, A.R., 1930. Fertilization and development without membrane formation in the egg of the sea urchin, Strongylocentrotus purpuratus. Protoplasma, 9, 9–17.

    Article  Google Scholar 

  • SHAPIRO, H., 1935. The validity of the centrifuge method for estimating aggregate cell volume in suspensions of the egg of the sea urchin, Arbacia punctulata. Biol. Bull., 68, 363–377.

    Article  Google Scholar 

  • SPOTTE, S.H., 1970. Fish and invertebrate culture, water management in closed systems. New York: Wiley-Interscience.

    Google Scholar 

  • TYLER, A., 1949. A simple, non-injurious method for inducing spawning of sea urchins and sand dollars. Collect. Net., 19, 19–20.

    Google Scholar 

  • TYLER, A., TYLER, B.S., 1966. The gametes; some procedure and properties. In: Physiology of Echinodermata (R.A. Boolootian, ed.), p. 639–682. New York: Interscience Publishers.

    Google Scholar 

  • VACQUIER, V.D., MAZIA, D., 1968. Twinning of sea urchin embryos by treatment with dithiothreitol. Exp. Cell Res., 52, 459–468.

    Article  PubMed  CAS  Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1975 Springer-Verlag Berlin · Heidelberg

About this chapter

Cite this chapter

Hinegardner, R. (1975). Care and Handling of Sea Urchin Eggs, Embryos, and Adults (Principally North American Species). In: Czihak, G. (eds) The Sea Urchin Embryo. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65964-5_2

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-65964-5_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-65966-9

  • Online ISBN: 978-3-642-65964-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics