Quantitative Nucleic Acid Histochemistry of the Yolk Sac Syncytium of Oviparous Teleosts: Implications for Hypotheses of Yolk Utilization

  • W. E. Bachop
  • F. J. Schwartz

Abstract

In the yolk-sac syncytium of the muskellunge embryo, Esox masquinongy ohioensis Kirtland, there are nuclei whose size and staining properties with basic dyes suggest that their chromatin content is greater than that of other embryonic nuclei (Bachop, 1965). This study is intended to test that supposition, using a histochemical test specific for one of the constituents of chromatin and using a microspectrophotometer to quantify the histochemical reaction in different nuclei relative to each other. The rationale of microphotometry is that insertion of the microscope in the optical train, and substitution of a dyed tissue on a slide for a dye solution in a cuvette, does not militate against using Beer’s law to deduce relative concentration of dye molecules from relative absorption of light (Pollister and Ornstein, 1959).

Keywords

Hydrolysis Toluene Peri Schiff Spectrophotometry 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bachop, W.E., 1965. Size, shape, number, and distribution of periblast nuclei in the muskellunge embryo (Clupeiformes: Esocidae). Trans. Am. microsc. Soc., 84, 80–86.CrossRefGoogle Scholar
  2. Bachop, W.E. and Price, J.W., 1971. Giant nuclei formation in the yolk-sac syncytium of the muskellunge, a bony fish (Salmoniformes: Esocidae: Esox masquinongy). J. Morph., 135, 239–246.CrossRefGoogle Scholar
  3. Deitch, A.D., 1966. Cytophotometry of nucleic acids, 327–354 pp. In: Introduction to Quantitative Cytochemistry. G.L. Wied (ed.). New York — London: Academic Press, 623 pp.Google Scholar
  4. Garcia, A.M. and Iorio, R., 1966. Potential sources of error in twowave length cytophotometry, 215–237 pp. In: Introduction to Quantitative Cytochemistry. G.L. Wied (ed.). New York — London: Academic Press, 623 pp.Google Scholar
  5. Hale, A.J., 1966. Feulgen microspectrophotometry and its correlation with other cytochemical methods, 183–199 pp. In: Introduction to Quantitative Cytochemistry. G.L. Wied (ed.). New York —London: Academic Press, 623 pp.Google Scholar
  6. Kasten, F.H., 1960. The chemistry of Schiff’s reagent. Intern. Rev. Cytol., X, 1–100.Google Scholar
  7. Korson, R., 1951. A microspectrophotometric study of red cell nuclei during pyknosis. J. exp. Med., 93, 121.PubMedCrossRefGoogle Scholar
  8. Leuchtenberger, C., 1958. Quantitative determination of DNA in cells by Feulgen microspectrophotometry, 219–278 pp. In: General Cytochemical Methods, Vol. I. J.F. Danielli (ed.). New York — London: Academic Press, 471 pp.Google Scholar
  9. Mendelsohn, M.L., 1958. The two-wave length method of microspectro-photometry. J. biophys. biochem. Cytol., 4, 407–424.PubMedCrossRefGoogle Scholar
  10. Mendelsohn, M.L. and Richards, B.M., 1958. A comparison of scanning and two-wave length microspectrophotometry. J. biophys. biochem. Cytol., 4, 707–709.PubMedCrossRefGoogle Scholar
  11. Moses, M., 1952. Quantitative optical techniques in the study of nuclear chemistry. Exp. Cell Res., Suppl., 2 75–102.Google Scholar
  12. Ornstein, L., 1952. Distributional error in microspectrophotometry. Lab. Invest., 1, 250–265.PubMedGoogle Scholar
  13. Patau, K., 1952. Absorption microphotometry of irregular shaped objects. Chromosoma, 5, 341–362.PubMedCrossRefGoogle Scholar
  14. Patau, K. and Swift, H., 1953. The DNA-content (Feulgen) of nuclei during mitosis in a root tip of onion. Chromosoma, 6, 149–169.PubMedCrossRefGoogle Scholar
  15. Pearse, A.G.E., 1960. Histochemistry Theoretical and Applied, 2nd ed. Boston: Little Brown and Co., 998 pp.Google Scholar
  16. Pollister, A.W. and Ornstein, L., 1955. Cytophotometric analysis in the visible spectrum, 3–71 pp. In: Analytical Cytology, R.G. Mellors (ed.). New York: McGraw-Hill Book Co.Google Scholar
  17. Pollister, A.W. and Ornstein, L., 1959. The photometric chemical analysis of cells, 431–518 pp. In: Analytical Cytology, 2nd ed., R.G. Mellors (ed.). New York: Mc-Graw-Hill Book Co., 543 pp.Google Scholar
  18. Swift, H. and Rasch, E., 1956. Microphotometry with visible light, 353–400 pp. In: Physical Techniques in Biological Research, Vol. III, G. Oster and A.W. Pollister (eds.). New York — London: Academic Press, 728 pp.Google Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1974

Authors and Affiliations

  • W. E. Bachop
    • 1
  • F. J. Schwartz
    • 2
  1. 1.Department of ZoologyClemson UniversityClemsonUSA
  2. 2.Institute of Marine SciencesThe University of North CarolinaMorehead CityUSA

Personalised recommendations