Culture and Preparation of Cells from Amniotic Fluid

  • Ulrich Wolf

Abstract

The analysis of fetal chromosomes is the most common occasion of prenatal diagnosis (reviews by Bergsma, 1971; Dorfman, 1972; Emery, 1973; Harris, 1972; Milunsky, 1973; Nadler, 1972), owing to the frequency of chromosomal aberrations, nearly all of which can be recognized. Prenatal cytogenetic diagnosis is particularly indicated in the following cases:
  1. 1.

    Above average maternal age (more than 35 or 40 years),

     
  2. 2.

    Familial chromosomal translocations (Robertsonian or otherwise),

     
  3. 3.

    Pregnancy following the birth of a child with a spontaneous chromosomal aberration,

     
  4. 4.

    Certain X-linked diseases.

     

Keywords

Transportation Citrate Penicillin Syringe Assure 

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References

  1. Abbo, G., Zellweger, H.: Prenatal determination of fetal sex and chromosomal complement. Lancet 1970 I, 216–217.Google Scholar
  2. Bergsma, D. (ed.): Intrauterine diagnosis. Birth defects: Original article series VII/5. The National Foundation — March of Dimes (U.S.A.), 1971.Google Scholar
  3. Dorfman, A. (ed.): Antenatal diagnosis. Chicago: Chicago Univ. Press 1972.Google Scholar
  4. Edwards, J. H.: Uses of amniocentesis. Lancet 1970 I, 608–609.Google Scholar
  5. Emery, A. E. H. (ed.): Antenatal diagnosis of genetic disease. Edinburgh London: Churchill Livingstone 1973.Google Scholar
  6. Fuchs, F.: Volume of amniotic fluid at various stages of pregnancy. Clin. Obstet. Gynec. 9, 449–460 (1966).PubMedCrossRefGoogle Scholar
  7. Fuchs, F., Riis, P.: Antenatal sex determination. Nature (Lond.) 177, 330 (1956).CrossRefGoogle Scholar
  8. Gray, C., Davidson, R. G., Cohen, M. M.: A simplified technique for the culture of amniotic fluid cells. J. Pediat. 79, 119–122 (1971).PubMedCrossRefGoogle Scholar
  9. Hahnemann, N.: Possibility of culturing foetal cells at early stages of pregnancy. Clin. Genet. 3, 286–293 (1972).PubMedCrossRefGoogle Scholar
  10. Harris, M. (ed.): Early diagnosis of human genetic defects. Fogarty Internat. Center Proc. No 6, 1972.Google Scholar
  11. Jonasson, J., Lindsten, J., Lundborg, R., Kissmeyer-Nielsen, F., Lamm, L.U., Petersen, G. B., Therkelsen, A. J.: HL-A antigens and heteromorphic characters of chromosomes in prenatal paternity investigation. Nature (Lond.) 236, 312–313 (1972).CrossRefGoogle Scholar
  12. Klinger, H. P., Schwarzacher, H. G.: The sex chromatin and heterochromatin bodies in human diploid and polyploid nuclei. J. biophys. biochem. Cytol. 8, 345 (1960).PubMedCrossRefGoogle Scholar
  13. Knörr-Gärtner, H., Härle, I.: A modified method of culturing human amniotic fluid cells for prenatal detection of genetic disorders. Humangenetik 14, 333–334 (1972).PubMedCrossRefGoogle Scholar
  14. Lee, C. L. Y., Gregson, N. M., Walker, S.: Eliminating red blood-cells from amniotic-fluid samples. Lancet 1970 II, 316–317.Google Scholar
  15. Leeuwen van, L., Jacoby, H., Charles, D.: Exfoliative cytology of amniotic fluid. Acta cytol. (Philad.) 9, 442 (1965).Google Scholar
  16. Lisgar, F., Gertner, M., Cherry, S., Hsu, L. Y., Hirschhorn, K.: Prenatal chromosome analysis. Nature (Lond.) 225, 280–281 (1970).CrossRefGoogle Scholar
  17. Macintyre, M. N.: Chromosomal problems of intrauterine diagnosis. In: Intrauterine diagnosis. Birth defects, Orig. art. ser. VII/5 (D. Bergsma, ed.) The Nat. Fdn. — March of Dimes 1971.Google Scholar
  18. Milunsky, A., Atkins, L., Littlefield, J. W.: Polyploidy in prenatal genetic diagnosis. J. Pediat. 79, 303–305 (1971).PubMedCrossRefGoogle Scholar
  19. Milunsky, A., Atkins, L., Littlefield, J. W.: Amniocentesis for prenatal genetic studies. Obstet, and Gynec. 40, 104–108 (1972).Google Scholar
  20. Milunsky, A.: The prenatal diagnosis of hereditary disorders. Charles C. Thomas: Springfield, Ill. 1973.Google Scholar
  21. Nadler, H. L.: Prenatal detection of genetic disorders. In: H. Harris, K. Hirschhorn eds., Advances in human genetics 3. New York-London: Plenum Press 1972.Google Scholar
  22. Nadler, H. L., Gerbie, A. B.: Role of amniocentesis in the intrauterine detection of genetic disorders. New Engl. J. Med. 282, 596–599 (1970).PubMedCrossRefGoogle Scholar
  23. Philip, J., Lebech, P., Niebuhr, E., Mikkelsen, M.: HMG-HCG stimulation, amniocentesis and prenatal chromosome analysis in a 14/21 translocation carrier with secondary amenorrhoea. Ugeskr. Laeg. 134, 1850–1852 (1972).PubMedGoogle Scholar
  24. Rook, A., Hsu, L. Y., Gertner, M., Hirschhorn, K.: Identification of Y and X chromosomes in amniotic fluid cells. Nature (Lond.) 230, 53 (1971).CrossRefGoogle Scholar
  25. Rüdiger, H. W., Wolff, R., Wendel, U., Passarge, E.: Enhancement of amniotic fluid cell growth in culture. Humangenetik, in press.Google Scholar
  26. Serr, D. M., Sachs, L., Danon, M.: Diagnosis of sex before birth using cells from the amniotic fluid. Bull. Res. Coun. Israel E 58, 137–138 (1955).Google Scholar
  27. Steele, M. W., Breg, W. R., Jr.: Chromosome analysis of human amniotic-fluid cells. Lancet 1966 I, 383–385.CrossRefGoogle Scholar
  28. Therkelsen, A. J., Petersen, G. B., Steenstrup, O. R., Jonasson, J., Lindsten, J., Zech, L.: Prenatal diagnosis of chromosome abnormalities. Acta pediat. scand. 61, 397–404 (1972).CrossRefGoogle Scholar
  29. Thiede, H. A., Creasman, W. T., Metcalfe, S.: Antenatal analysis of human chromosomes. Amer. J. Obstet. Gynec. 94, 569 (1966).Google Scholar
  30. Valenti, C., Kehaty, T.: Culture of cells obtained by amniocentesis. J. Lab. clin. Med. 73, 355–358 (1969).PubMedGoogle Scholar
  31. Votta, R. A., Gagneten, C. B. de, Parada, O., Giulietti, M.: Cytologic study of amniotic fluid in pregnancy. Amer. J. Obstet. Gynec. 102, 571 (1968).PubMedGoogle Scholar
  32. Wahlstrøm, J., Brosset, A., Bartsch, F.: Viability of amniotic cells at different stages of gestation. Lancet 1970 II, 1037.Google Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1974

Authors and Affiliations

  • Ulrich Wolf

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