Culture and Preparation of Cells from Amniotic Fluid

  • Ulrich Wolf


The analysis of fetal chromosomes is the most common occasion of prenatal diagnosis (reviews by Bergsma, 1971; Dorfman, 1972; Emery, 1973; Harris, 1972; Milunsky, 1973; Nadler, 1972), owing to the frequency of chromosomal aberrations, nearly all of which can be recognized. Prenatal cytogenetic diagnosis is particularly indicated in the following cases:
  1. 1.

    Above average maternal age (more than 35 or 40 years),

  2. 2.

    Familial chromosomal translocations (Robertsonian or otherwise),

  3. 3.

    Pregnancy following the birth of a child with a spontaneous chromosomal aberration,

  4. 4.

    Certain X-linked diseases.



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© Springer-Verlag Berlin · Heidelberg 1974

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  • Ulrich Wolf

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