Skip to main content
SpringerLink
Log in

Interaction of Poliovirus-Specific RNAs with HeLa Cells and E. coli

  • Conference paper
Current Topics in Microbiology and Immunology / Ergebnisse der Mikrobiologie und Immunitätsforschung

  • 60 Accesses

Abstract

General aspects of nucleic acid uptake by mammalian cells have been the subject of several reviews during the last few years (Pagano, 1970; Bhargava and Shanmugam, 1971; Dubes, 1971; Ryser, 1967). These reviews covered methods used for the infection of cells by viral nucleic acids as well as interaction of mammalian cells with non-viral nucleic acids.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Alexander, H. E., Koch, G., Mountain, I. M., Van Damme, O.: Infectivity of ribonucleic acid from poliovirus in human cell monolayers. J. exp. Med. 108, 493 (1958).

    Article  PubMed  CAS  Google Scholar 

  • Amstey, M. S.: Enhancement of polio-RNA infectivity with dimethylsulfoxide, Fed. Proc. 25, 492 (1966).

    Google Scholar 

  • August, J. T., Ortiz, P. J., Hurwitz, J.: Ribonucleic acid-dependent ribonucleotide incorporation. J. biol. Chem. 237, 3786–3793 (1962).

    PubMed  CAS  Google Scholar 

  • Bhargava, P. M., Shanmugam, G.: Uptake of nonviral nucleic acids by mammalian cells. Progress in nucleic acid research and molecular biology, vol. 11, p. 1030–159, New York: Academic Press 1971.

    Google Scholar 

  • Bishop, J. M., Koch, G.: Purification and characterization of poliovirus induced infectious double-stranded RNA. Biol. Chem. 242, 1736 (1967).

    CAS  Google Scholar 

  • Bishop, J. M., Quintrell, N., Koch, G.: Poliovirus double-stranded RNA; Inactiva-tion by ultraviolet light. J. Mol. Biol. 24, 125 (1967).

    Article  CAS  Google Scholar 

  • Bishop, J. M., Koch, G.: Infectious replicative intermediate of poliovirus: Purification and characterization. Virology 37, 521 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Bishop, J. M., Levintow, L.: Nucleic acid as the carrier of viral activity. In: Progress in medical virology (J. L. Melnick, ed.), vol. 13, p. 1–65, Basel: Karger 1971.

    Google Scholar 

  • Borgert, K., Koschel, K., Taeuber, E.L., Wecker, E.: Effect of inactivation by hydroxy lamine on early functions of poliovirus. J. Virol. 8, 1–6 (1971).

    PubMed  CAS  Google Scholar 

  • Borriss, E.: Wechselbeziehungen zwischen 32P-markierten Ribonucleinsäuren und suspendierten Gewebekulturzellen. III. Einfluß von Versen, Mg2+ und Protamin. Z. Naturforsch. 20, 752 (1965).

    Google Scholar 

  • Borriss, E., Koch, G.: Wechselbeziehungen zwischen 32P-markierten Ribonukleinsäuren und suspendierten Gewebekulturzellen. I. Adsorption. Z. Naturforsch. 19, 32 (1964 a).

    CAS  Google Scholar 

  • Borriss, E., Koch, G.: Wechselbeziehungen zwischen 32P-markierten Ribonukleinsäuren und suspendierten Gewebekulturzellen. II. Übergang der RNS von einem RNase-sensitiven in einen RNase-resistenten Zustand. Z. Naturforsch. 19, 686 (1964 b).

    Google Scholar 

  • Borriss, E., Shu, S. L., Koch, G.: Wechselbeziehungen zwischen 32P-markierten Ribonukleinsäuren und suspendierten Gewebekulturzellen. IV. Schicksal der RNS nach Adsorption und Penetration. Z. Naturforsch. 20, 759 (1965).

    Google Scholar 

  • Breindl, M.: The structure of heated poliovirus particles, J. gen. Virol 11, 147 (1971a).

    Article  PubMed  CAS  Google Scholar 

  • Breindl, M.: VP 4, the D-reactive part of poliovirus, Virology 46, 962–964 (1971b).

    Article  PubMed  CAS  Google Scholar 

  • Breindl, M., Koch, G.: Competence of suspended HeLa Cells for infection by inactivated poliovirus particles and by isolated viral RNA. Virology 48, 136–144 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Colter, J. S., Bird, H. E.L., Moyer, A. W., Brown, R. A.: Infectivity of ribonucleic acid isolated from virus infected tissues. Virology 4, 522 (1957).

    Article  PubMed  CAS  Google Scholar 

  • Dubes, G. R.: Methods for transfecting cells with nucleic acids of animal viruses: a Review, Experentia (Basel), Suppl. 16, ed. 1971.

    Google Scholar 

  • Dubes, G. R., Faas, F. H., Kelly, D. G., Chapín, M., Lamb, R. D., Lucas, T. A.: A study of the facilitations of infection with surviving poliovirus units. J. infect. Dis. 114, 346 (1964).

    Article  PubMed  CAS  Google Scholar 

  • Drzeniek, R., Bilello, P.: Reconstitution of poliovirus. Biochem. Biophys. Res. Comm. 46, 719 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Engelhardt, D. L., Zinder, N. D.: Host-dependent mutants of the bacteriophage f2: III. Infective RNA, Virology 23, 582 (1964).

    Article  PubMed  CAS  Google Scholar 

  • Fox, C. F., Robinson, W. S., Haselkorn, R., Weiss, S. B.: Enzymatic synthesis of ribonucleic acid. III. The ribonucleic acid-primed synthesis of ribonucleic acid with Micrococcus lysodeikticus ribonucleic acid polymerase. J. biol. Chem. 239, 186–193 (1964).

    PubMed  CAS  Google Scholar 

  • Fraenkel-Conrat, H., Singer, B., Williams, R. C.: Infectivity of viral nucleic acid. Biochem. biophys. Acta (Amst.) 25, 87 (1957).

    Article  PubMed  CAS  Google Scholar 

  • Franklin, R. M.: Purification and properties of the replicative intermediate of the RNA bacteriophage R 17. Proc. nat. Acad. Sci. (Wash.) 55, 1504 (1966).

    Article  PubMed  CAS  Google Scholar 

  • Franklin, R. M., Wecker, E.: Inactivation of some animal viruses by hydroxylamine and the structure of ribonucleic acid. Nature (Lond.) 184, 343–345 (1959)

    Article  PubMed  CAS  Google Scholar 

  • Gierer, A., Schramm, G.: Die Infektivitaet der Nucleinsäure aus Tabakmosaikvirus. Z. Naturforsch. 11b, 138. (1956).

    CAS  Google Scholar 

  • Havliza, D., Koch, G.: Complex formation between poliovirus RNA and polycations. Arch. Biochem. Biophys. 147, 85–91 (1971).

    Article  PubMed  CAS  Google Scholar 

  • Hershey, A. D., Chase, M.: Independent functions of viral protein and nucleic acid in growth of bacteriophage. J. gen. Physiol. 36, 39 (1952).

    Article  PubMed  CAS  Google Scholar 

  • Jachertz, D.: Replicase, an enzyme for multiplication of informational RNA. Behring-werke-Mitt., H. 49, 147 (1969).

    Google Scholar 

  • Jacob, S. W., Bischel, M., Herschler, J.L.: Dimethylsulfoxide: Effects on the permeability of biologic membranes. Curr. ther. Res. 6, 134–135 (1964).

    PubMed  CAS  Google Scholar 

  • Katz, L., Penman, S.: The solvent denaturation of doublestranded RNA from polio-virus-infected HeLa cells. Biochem. biophys. Res. Comm. 23, 557 (1966).

    Article  PubMed  CAS  Google Scholar 

  • Kirby, K. S.: Isolation and fractionation of nucleic acids. Davidson, J. N., Cohn, W. E.: Progress in nucleic acid research and molecular biology, vol. 3, p. 1. New York-London: Acad. Press 1964.

    Chapter  Google Scholar 

  • Klingler, E. A., Jr., Chapin, M., Dubes, G. R.: Relationship between inactivation of poliovirus by phenol and appearance of ribonuclease-labile infectivity. Proc. Soc. exp. Biol. (N. Y.) 101, 829 (1959).

    CAS  Google Scholar 

  • Koch, G.: Influence of assay conditions on infectivity of heated poliovirus. Virology 12, 601 (1960).

    Article  PubMed  CAS  Google Scholar 

  • Koch, G.: Zur unterschiedlichen Infektiosität von isolierter Ribonukleinsäure und intaktem Poliovirus in der Gewebekultur. Z. Naturforsch. 15B, 656 (1960).

    Google Scholar 

  • Koch, G.: Zum Ablauf der Invasion von Virusnukleinsäure in Gewebekulturzellen. Z. Naturforsch. 18b, 899 (1963).

    Google Scholar 

  • Koch, G.: Differential effect of phleomycin on the infectivity of poliovirus and polio-virus-induced ribonucleic acids. J. Virol. 8, 28 (1971a).

    PubMed  CAS  Google Scholar 

  • Koch, G.: Stability of polycation-induced cell competence for infection by viral ribonucleic acid. Virology 45, 841 (1971b).

    Article  PubMed  CAS  Google Scholar 

  • Koch, G., Bishop, J. M.: The effect of polycations on the interaction of viral RNA with mammalian cells: Studies on the infectivity of single and double-stranded poliovirus RNA. Virology 35, 9–17 (1968).

    Article  PubMed  CAS  Google Scholar 

  • Koch, G., Bishop, J. M., Wiegers, K. J., Lempidakis, G., Bodelschwingh, H. von: The replication of poliovirus. Proc. of the 7th European Symposium of Poliomyelitis and Allied Diseases, Bukarest, 1970, p. 429–439.: Publishing House of the Academy of the Socialist Republic of Romania.

    Google Scholar 

  • Koch, G., Koening, S., Alexander, H. E.: Quantitative studies on the infectivity of ribonucleic acid from partially purified and highly purified poliovirus preparations. Virology 10, 329–343 (1960).

    Article  PubMed  CAS  Google Scholar 

  • Koch, G., Quintrell, N., Bishop, J. M.: An agar-cell suspension plaque assay for isolated viral RNA. Biochem. biophys. Res. Commun. 24, 304–309 (1966).

    Article  PubMed  CAS  Google Scholar 

  • Koch, G., Quintrell, N., Bishop, J. M.: Differential Effect of Actinomycin D on the Infectivity of single and double-stranded Poliovirus RNA. Virology 31, 388 (1967).

    Article  PubMed  CAS  Google Scholar 

  • Koch, G., Vollersten, I.: Interaction of viral RNA with E. coli, II. Synthesis of poliovirus specific RNA directed by poliovirus RF-RNA. Arch. Biochem. Biophys 152, 475 (1972a).

    Article  PubMed  CAS  Google Scholar 

  • Koch, G., Vollersten, I.: Interaction of viral RNA with E. coli, III. Synthesis of poliovirus specific RNA directed by isolated poliovirus RNA. Arch. Biochem. Biophys 163, 823 (1972b).

    Article  Google Scholar 

  • Koch, G., Vollertsen, I.: RNA-Dependent RNA polymerase from E. coli. 8th FEBS Meeting, August 20–25, 1972 (c) — Abstract.

    Google Scholar 

  • Krakow, J. S., Ochoa, S.: Ribonucleic acid polymerase of azotobacter Vinelandii, I. Priming by Polyribonucleotides. Proc. Biochem. 19, 88 (1963).

    Google Scholar 

  • Kubinski, H., Heise, M.: Spontaneous Formation of Stable Complexes between Nucleic Acids and Proteins. Abstr. Annual Meet. Biophys. Soc. 14th, Baltimore, Md., 1717 (1970).

    Google Scholar 

  • Kubinski, H., Kasper, C. B.: Interactions between carcinogens and microsomal membrane: Change in membrane density and in its ability to bind nucleic acids. Science 171, 201 (1971).

    Article  PubMed  CAS  Google Scholar 

  • Kubinski, H., Koch, G.: Regulation of the synthesis of various ribonucleic acids in animal cells. Biochem. biophys. Res. Commun. 22, 346 (1966).

    Article  PubMed  CAS  Google Scholar 

  • Lempidakis, G. A., Koch, G.: Interaction of viral RNA with E. coli. I. Polycation augmented adsorption of poliovirus induced double-stranded RNA. Arch. Biochem. Biophys. (1972) (in press).

    Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the folin phenol reagent. J. biol. Chem. 193, 265 (1951)

    PubMed  CAS  Google Scholar 

  • Ludwig, E. H., Smull, C. E.: Infectivity of histone-poliovirus ribonucleic acid preparations. J. Bact. 85, 1334 (1963).

    PubMed  CAS  Google Scholar 

  • Mandel, B.: The extraction of ribonucleic acid form poliovirus by treatment with sodium dodecylsulfate. Virology 32, 360 (1964).

    Article  Google Scholar 

  • Mans, R. J., Novelli, G. D.: Measurement of the incorporation of radioactive amino acids into protein by a filter-paper dish method. Arch. Biochem. Biophys. 94, 48 (1961).

    Article  CAS  Google Scholar 

  • Miller, P. A., Milstrey, K. B., Trown, P. W.: Specific inhibition of viral, ribonucleic acid replication by gliotoxin. Science 159, 431 (1968).

    Article  PubMed  CAS  Google Scholar 

  • Mittelstaedt, R., Koch, G.: Poliovirus induced infectious double-stranded RNA: Effect of RNA degrading enzymes (to be published in 1974).

    Google Scholar 

  • Miura, K. I., Muto, A.: Lack of messenger RNA activity of a double-stranded RNA. Arch. Biochem. Biophys. 108, 707 (1965).

    CAS  Google Scholar 

  • Nakamoto, T., Weiss, S. B.: The biosynthesis of RNA; priming by polyribonucleotides. Proc. Biochem. 48, 880 (1962).

    CAS  Google Scholar 

  • Neuhoff, V., Schill, W., Jacherts, D.: Nachweis einer RNA abhängigen RNA Replicase aus immunologisch kompetenten Zellen durch Mikro-Disk-Elektrophorese. Hoppe Seylers Z. physiol. Chem., 351, 157 (1970).

    Article  PubMed  CAS  Google Scholar 

  • Oberg, B.: Biochemical and biological characteristics of carbethoxylated poliovirus and viral RNA. Biochem. biophys. Acta (Amst.) 204, 430–440 (1970).

    PubMed  CAS  Google Scholar 

  • Oppermann, H., Koch, G.: Kinetics of poliovirus replication in HeLa Cells infected by isolated RNA, BBRC 52, 635–640 (1973).

    PubMed  CAS  Google Scholar 

  • Pagano, J. S.: Biologic activity of isolated viral nucleic acids. Prog. Med. Virol. 12, 1–48. Basel-München-New York: Karger 1970.

    Google Scholar 

  • Philipson, L.: Degradation of poliovirus by mercurials. Biochem. biophys. Res. Commun. 17, 352 (1964).

    Article  CAS  Google Scholar 

  • Ravin, A. W.: Bacterial Genetics. Ann. Rev. Microbiol. 12, 309 (1958).

    Article  CAS  Google Scholar 

  • Robertson, H. D.: Enzymatic synthesis of bacteriophage f1 DNA: RNA hybrid and double-stranded RNA. Nature (Lond.) New Biol. 229, 169 (1971).

    CAS  Google Scholar 

  • Ryser, H. J.: Studies on protein uptake by isolated tumor cells. III. Apparent stimulation due to pH, hypertonicity, polycations, or dehydration and their relation to the enhanced penetration of infectious nucleic acids. J. Cell Biol. 32, 737–750 (1967).

    Article  PubMed  CAS  Google Scholar 

  • Ryser, H. J., Hancock, R.: Histones and basic polyamino acids stimulate the uptake of albumin by tumor cells in culture. Science 150, 501–503 (1965).

    Article  PubMed  CAS  Google Scholar 

  • Saborio, J. L., Koch, G.: Inhibition of protein synthesis in HeLa cells by dimethyl-sulfoxide. (insert: J. Mol. Biol., in press).

    Google Scholar 

  • Saborio, J. L., Wiegers, K. J., Koch, G.: Inhibition of protein synthesis in HeLa cells by DEAE-dextran (to be published).

    Google Scholar 

  • Shatkin, A. J., Banerjee, A. K.: In vitro transcription of double-stranded RNA by reovirus-associated RNA polymerase. Cold Spring Harb. Symp. Quant. Biology 35 (1970).

    Google Scholar 

  • Schaffer, F. L.: Physical and chemical properties and infectivity of RNA from animal viruses. Cold Spr. Harbor Symp. quant. Biol. 27, 89 (1962).

    CAS  Google Scholar 

  • Schaffer, F. L., Mattern, C. F. T.: Infectivity and physiochemical studies on RNA preparations from highly purified poliomyelitis and Coxsackie viruses. Fed. Proc. 18, 317 (1959).

    Google Scholar 

  • Schuster, H., Schramm, G., Zillig, W.: Die Struktur der Ribonukleinsäure aus Tabakmosaikvirus. Z. Naturforsch. 11b, 339 (1956).

    Google Scholar 

  • Summers, D. F., Maizel, J. V., Jr., Darnell, J. E. Jr.: Evidence in poliovirus infected HeLa cells. Proc. nat. Acad. Sci. (Wash.) 54, 505 (1965).

    Article  PubMed  CAS  Google Scholar 

  • Tobia, A. M., Schildkraut, C. L., Maio, J. J.: Desoxyribonucleic acid replication in synchronized cultured mammalian cells. I. Time of synthesis of molecules of different average guanine and cytosine content. J. molec. Biol. 54, 499–515 (1970).

    Article  PubMed  CAS  Google Scholar 

  • Tovell, D. R., Colter, J. S.: Observations on the assay of infectious viral ribonucleic acid: Effect of DMSO and DEAE dextran. Virology 32, 84–92 (1967).

    Article  PubMed  CAS  Google Scholar 

  • Trown, P. W., Billelo, T. A.: Mechanism of action of Gliotoxin, Elimination of activity by sulfhydryl compounds. Antimicrobial Agents and Chemotherapy (in press 1972).

    Google Scholar 

  • Wecker, E.: Virus and nucleic acid. Ergebn. Mikrobiol. 35, 1 (1962).

    CAS  Google Scholar 

  • Wehrli, W., Knusel, F., Schmid, K., Staehelin, M.: Interaction of rifampicin with bacterial RNA polymerase. Proc. nat. Acad. Sci. (Wash.) 61, 667 (1968).

    Article  PubMed  CAS  Google Scholar 

  • Wentzky, P., Koch, G.: Influence of polycations on the interaction between poliovirus multistranded ribonucleic acid and HeLa cells. Virology 35, 35 (1971)-

    Google Scholar 

  • Wiegers, K. J., Koch, G.: Interaction of poliovirus-induced double-stranded RNA with HeLa cells. Arch. Biochem. Biophys. 148, 89–96 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Zomzely, C. E., Roberts, S., Peache, S.: Isolation of RNA with properties of messenger RNA from cerebral polyribosomes. Proc. nat. Acad. Sci. (Wash.) 67, 644 (1970).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Roche Institute of Molecular Biology, Nutley, N.J., 07110, USA

    Gebhard Koch

Authors
  1. Gebhard Koch
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Basle, Switzerland

    W. Arber  & N. K. Jerne  & 

  2. Freiburg, Germany

    R. Haas

  3. Philadelphia, USA

    W. Henle , P. Koldovský  & H. Koprowski ,  & 

  4. Martinsried, Germany

    P. H. Hofschneider

  5. Copenhagen, Denmark

    O. Maaløe

  6. Gießen, Germany

    R. Rott

  7. Wilhelmshaven, Germany

    H. G. Schweiger

  8. Rehovot, Israel

    M. Sela

  9. Prague, Czech Republic

    L. Syruček

  10. Seattle, USA

    P. K. Vogt

  11. Würzburg, Germany

    E. Wecker

Rights and permissions

Reprints and permissions

Copyright information

© 1973 Springer-Verlag, Berlin · Heidelberg

About this paper

Cite this paper

Koch, G. (1973). Interaction of Poliovirus-Specific RNAs with HeLa Cells and E. coli. In: Arber, W., et al. Current Topics in Microbiology and Immunology / Ergebnisse der Mikrobiologie und Immunitätsforschung. Current Topics in Microbiology and Immunology / Ergebnisse der Mikrobiologie und Immunitätsforschung, vol 62. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65772-6_4

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-65772-6_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-65774-0

  • Online ISBN: 978-3-642-65772-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation