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Immunological Recognition of Polynucleotide Structure

  • F. Lacour
  • E. Nahon-Merlin
  • M. Michelson
Conference paper
Part of the Current Topics in Microbiology and Immunology / Ergebnisse der Mikrobiologie und Immunitätsforschung book series (CT MICROBIOLOGY, volume 62)

Abstract

Since the discovery of polynucleotide Phosphorylase in 1955 and the availability of synthetic polynucleotides, a vast number of physical chemical studies have been pursued with such polymers, in particular by use of optical techniques. (For review see Michelson et al., 1967). It was early shown that complementary polynucleotides readily interacted to give DNA type double-stranded helical complexes. Since then, secondary structure in single strands, though less well defined, has been amply explored, as well as formation of triple-stranded complexes under certain conditions. All of these “natural” polymers and polymer complexes adopt a right-handed helical conformation in aqueous solution, and though left-handed structures have been proposed (these do indeed exist in polynucleotides containing L-ribose instead of D-ribose), no rigorous evidence of their existence in natural polynucleotides has been provided. Although the overall geometry is thus right-handed helical, a variety of geometrically distinct forms are known to exist, for example the A, B and C forms of DNA and the A form of double-helical RNA. In addition, chemical and physical studies have shown that synthetic polynucleotide complexes possess different absolute geometries in terms of double helix, and indeed two extreme DNAs, poly dAT from crab, and DNA from Micrococcus lysodeikticus (72% GC) show quite different structures.

Keywords

Systemic Lupus Erythematosus Patient Immune Seron Complex Poly Homologous Antigen Immunological Recognition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag, Berlin · Heidelberg 1973

Authors and Affiliations

  • F. Lacour
  • E. Nahon-Merlin
  • M. Michelson
    • 1
    • 2
  1. 1.Laboratoire d’Immunologie Groupe de Recherche n° 8 du C.N.R.S.Institut Gustave-Roussy 94-VillejuifParis 5emeFrance
  2. 2.Institut de Biologie Physico-Chimique 13Paris 5emeFrance

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