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Bioassay for Brain Hormone

  • Masatoshi Kobayashi
  • Masayoshi Yamazaki

Abstract

In Bombyx silkworms diapause does not occur in the pupal stage, and silkworm pupae become moths in about 12 days following pupation at 25° C. However, in an experimental study on the function of the brain during the pupal stage, Kobayashi (1955; 1957; Kobayashi and Kirimura, 1958; Kobayashi and Yamashita, 1959) found that when pupal brains were extirpated immediately after pupation, most did not show any sign of imaginai differentiation and that these brainless pupae proved to be one of the most suitable animals for the assay of brain hormone. Each pupa treated in this way was called a Dauer-pupa, following the nomenclature of Wigglesworth for Dauer-larva.

Keywords

Abdominal Segment Pupal Stage Larval Brain Fresh Brain Melted Paraffin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer-Verlag New York Inc. 1974

Authors and Affiliations

  • Masatoshi Kobayashi
  • Masayoshi Yamazaki

There are no affiliations available

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