Bioassay for Brain Hormone
In Bombyx silkworms diapause does not occur in the pupal stage, and silkworm pupae become moths in about 12 days following pupation at 25° C. However, in an experimental study on the function of the brain during the pupal stage, Kobayashi (1955; 1957; Kobayashi and Kirimura, 1958; Kobayashi and Yamashita, 1959) found that when pupal brains were extirpated immediately after pupation, most did not show any sign of imaginai differentiation and that these brainless pupae proved to be one of the most suitable animals for the assay of brain hormone. Each pupa treated in this way was called a Dauer-pupa, following the nomenclature of Wigglesworth for Dauer-larva.
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