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Functional Characterization of Five Initiation Factors for Mammalian Protein Synthesis

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Regulation of Transcription and Translation in Eukaryotes

Abstract

Although there has been rapid progress in the separation, purification and partial functional characterization of several protein factors involved in mammalian polypeptide chain initiation from rabbit reticulocytes [1–9], the mechanism of eukaryotic chain initiation is still not clear. In our earlier work [10] we used well-defined ribosomes (purified 60 S and 40 S subunits) whose structural and functional integrity was shown by their capacity to participate quantitatively and efficiently in the in vitro translation of rabbit globin mRNA.

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References

  1. Shafritz, D.A., Anderson, W.F.: Nature (Lond.) 227, 918 (1970).

    Article  CAS  Google Scholar 

  2. Prichard, P. M., Gilbert, J. M., Shafritz. D. A., Anderson, W. F.: Nature (Lond.) 226, 511 (1970).

    Article  CAS  Google Scholar 

  3. Shafritz, D. A., Laycock, D. G., Anderson, W. F.: Proc. nat. Acad. Sci. (Wash.) 68, 496 (1971).

    Article  CAS  Google Scholar 

  4. Crystal, R. G., Shafritz, D. A., Prichard, P. H., Anderson, W. F.: Free. nat. Acad. Sci. (Wash.) 68, 1810 (1971).

    Article  CAS  Google Scholar 

  5. Shafritz, D. A., Laycock, D. G., Crystal, R. G., Anderson, W. F.: Proc. nat. Acad. Sci. (Wash.) 68, 2246 (1971).

    Article  CAS  Google Scholar 

  6. Prichard, P. M., Picciano, D. J., Laycock, D. G., Anderson, W. F.: Proc. nat. Acad. Sci. (Wash.) 68, 2752 (1971).

    Article  CAS  Google Scholar 

  7. Crystal, R. G., Anderson, W. F.: Proc. nat. Acad. Sci. (Wash.) 69, 106 (1972).

    Article  Google Scholar 

  8. Shafritz, D. A., Prichard, P. M., Gilbert, J. M., Merrick, W. C., Anderson, W. F.: Proc. nat. Acad. Sci. (Wash.) 69, 983 (1972).

    Article  CAS  Google Scholar 

  9. Crystal, R. G., Nienhuis, A. W., Prichard, P. M., Picciano, D., Elson, N. A., Merrick, W. C., Graf, H., Shafritz, D. A., Laycock, D. G., Last, J.A., Anderson, W.F.: FEBS Letters 24, 310 (1972).

    Article  PubMed  CAS  Google Scholar 

  10. Schreier, M.H., Staehelin, T.: J. molec. Biol. 73, 329 (1973).

    Article  PubMed  CAS  Google Scholar 

  11. Schreier, M. H., Staehelin, T.: Nature (Lond.) New Biol. 242, 35 (1973).

    CAS  Google Scholar 

  12. Marcus, A.: J. biol. Chem. 245, 955 (1970).

    PubMed  CAS  Google Scholar 

  13. Marcus, A.: J. biol. Chem. 245, 962 (1970).

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Basle Institute for Immunology, Basle, Switzerland

    Max H. Schreier & Theophil Staehelin

Authors
  1. Max H. Schreier
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  2. Theophil Staehelin
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Editors and Affiliations

  1. Institut für Molekulare Genetik, Zoologisches Institut der Universität, D-6900, Heidelberg, Neuenheimer Feld, Federal Republic of Germany

    E. K. F. Bautz

  2. Physiologisch-Chemisches Institut der Universität, D-3550, Marburg, Federal Republic of Germany

    P. Karlson

  3. Physiologisch-Chemisches Institut der Universität, D-8520, Erlangen, Federal Republic of Germany

    H. Kersten

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© 1973 Springer-Verlag Berlin · Heidelberg

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Schreier, M.H., Staehelin, T. (1973). Functional Characterization of Five Initiation Factors for Mammalian Protein Synthesis. In: Bautz, E.K.F., Karlson, P., Kersten, H. (eds) Regulation of Transcription and Translation in Eukaryotes. Colloquium der Gesellschaft für Biologische Chemie 26.–28. April 1973 in Mosbach/Baden, vol 24. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65725-2_18

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  • DOI: https://doi.org/10.1007/978-3-642-65725-2_18

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-65727-6

  • Online ISBN: 978-3-642-65725-2

  • eBook Packages: Springer Book Archive

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