The Mechanism of Subunit Interaction as a Key to the Understanding of Ribosome Function

  • Hans Noll
  • Markus Noll
  • Bern Hapke
  • Gerbrand van Dieijen
Part of the Colloquium der Gesellschaft für Biologische Chemie 26.–28. April 1973 in Mosbach/Baden book series (MOSBACH, volume 24)

Abstract

The most intriguing feature of the ribosome is its highly asymmetrical construction of two unequal subunits that, in the case of prokaryotic organisms, are described by their 30 S and 50 S sedimentation values. Although the pioneering work of Tissières and Watson [1] established that Mg2+ ions were essential to maintain the associated state and hinted at the existence of a dynamic equilibrium, a rather static view of ribosome structure dominated the thinking for nearly a decade. Perhaps consonant with the prevailing mores of the time, it was taken for granted that the union between the two subunits was a marriage for life. This vision seemed to satisfy an inner need, for how else could it be explained that the scientists would suppress their natural curiosity and refrain from a closer scrutiny of the relationship between the subunits ? However, the less reverent and more open climate of the sexual revolution and women’s lib was not going to bypass the ribosome, and soon representatives of the younger generation [2] boldly proclaimed that ribosomes not engaged in protein synthesis normally existed as free subunits that would enter a temporary union only for the purpose of creating a new polypeptide chain. Although this radical view subsequently turned out to be untenable, by removing an old taboo it led to fresh experiments and marked the beginning of a more dynamic view of the ribosome.

Keywords

Formaldehyde Codon Sedimentation Electrophoresis Polyacrylamide 

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References

  1. 1.
    Tissieres, A., Watson, J.D.: Nature (Lond.) 182, 778 (1958).CrossRefGoogle Scholar
  2. 2.
    Mangiarotti, G., Sahlessinger, D.: J. molec. Biol. 29, 395 (1967).CrossRefGoogle Scholar
  3. 3.
    Kaempfer, R., Meselson, M., Raskas, H.J.: J. molec. Biol. 31, 277 (1968).PubMedCrossRefGoogle Scholar
  4. 4.
    Davis, B.D.: Nature (Lond.) 231, 153 (1971).CrossRefGoogle Scholar
  5. 5.
    Noll, M., Hapke, B., Schreier, M. H., Noll, H.: J. molec. Biol. 75, 281 (1973).PubMedCrossRefGoogle Scholar
  6. 6.
    Lengyel, P., Söll, D.: Bact. Rev. 33, 264 (1969).PubMedGoogle Scholar
  7. 7.
    Noll, M., Noll, H.: Nature (Lond.) New Biol. 238, 225 (1972).CrossRefGoogle Scholar
  8. 8.
    Zitomer, R.S., Flaks, J. G.: J molec. Biol. 71, 263 (1972).PubMedCrossRefGoogle Scholar
  9. 9.
    Hapke, B., Noll, H.: Submitted for publication.Google Scholar
  10. 10.
    Noll, M.: Thesis, Northwestern University, 1972.Google Scholar
  11. 11.
    Noll, M., Hapke, B., Noll, H.: J. molec. Biol. 80 (1973)Google Scholar
  12. 12.
    Noll, M., Noll, H.: Submitted for publication.Google Scholar
  13. 13.
    Noll, H.: Vierteljahresschr. Naturforsch. Gesell. Zürich 116, 377 (1971).Google Scholar
  14. 14.
    Spirin, A. S.: In: Currents in Modern Biology, Vol. 2, p. 115. Amsterdam: North-Holland Publ. Co. 1968.Google Scholar
  15. 15.
    Schreier, M H, Noll, H.: Proc. nat. Acad. Sci. (Wash.) 68, 805 (1971).CrossRefGoogle Scholar
  16. 16.
    Steitz, J. A.: Nature (Lond.) 224, 957 (1969).CrossRefGoogle Scholar
  17. 17.
    Housman, D., Gillespie, D., Lodish, H. F.: J. molec. Biol. 65, 163 (1972).PubMedCrossRefGoogle Scholar
  18. 18.
    Dahlberg, A. E., Dingman, C. W., Peacock, A.C.: J. molec. Biol. 41, 139 (1969).PubMedCrossRefGoogle Scholar
  19. 19.
    Van Diggelen, O. P, Oosmom, H., Bosch, L.: FEBS Letters 19, 115 (1971).PubMedCrossRefGoogle Scholar
  20. 20.
    Kurland, C.G.: J. molec. Biol. 18, 90 (1966).PubMedCrossRefGoogle Scholar
  21. 21.
    Kurland, C. G.: Ann. Rev. Biochem. 41, 377 (1972).PubMedCrossRefGoogle Scholar
  22. 22.
    Noll, H.: In: Symp. Fundamental Cancer Research; Univ. Texas M.D. Anderson Hospital and Tumor Inst., Houston, Texas, p. 67. Baltimore: William & Wilkens Co. 1965.Google Scholar
  23. 23.
    Herzog, H., Ghysen, A., Bollen, A.: FEES Letters 15, 291 (1971).CrossRefGoogle Scholar
  24. 24.
    Spirin, A.S.: FEBS Letters 14, 349 (1971).PubMedCrossRefGoogle Scholar
  25. 25.
    Subramanian, A. R., Davis, B. D.: Proc. nat. Acad. Sci. (Wash.) 68, 2543 (1971).CrossRefGoogle Scholar
  26. 26.
    Zamm, A., Miskin, R., Elson, D.: J. molec. Biol. 60, 347 (1971).CrossRefGoogle Scholar
  27. 27.
    Schreier, M. H., Noll, H.: Nature (Lond.) 227, 128 (1970).CrossRefGoogle Scholar
  28. 28.
    Lodish, H.F.: Nature (Lond.) 226, 705 (1970).CrossRefGoogle Scholar
  29. 29.
    Chuang, D.M., Simpson, M.V.: Proc. nat. Acad. Sci. (Wash.) 68, 1474 (1971).CrossRefGoogle Scholar
  30. 30.
    Kaempfer, R.: Nature 228, 534 (1970).PubMedCrossRefGoogle Scholar
  31. 31.
    Garcia-Patrone, M., Gonzalez, N. S., Algranati, I. D.: Proc. nat. Acad. Sci. (Wash.) 68, 2822 (1971).CrossRefGoogle Scholar
  32. 32.
    Van Dieijen, G., Noll, H.: to be published.Google Scholar
  33. 33.
    Marsh, R. D., Parmeggianai, A.: Proc. nat. Acad. Sci. (Wash.) 70, 151 (1973).CrossRefGoogle Scholar
  34. 34.
    Highland, J. H., Ochsner, E., Gordon, J., Bodley, J., Hasenbank, R., Stoeffler, G.: submitted for publication.Google Scholar
  35. 35.
    Wittmann, H. G., Stoeffler, G.: In: Bosch, L.: The Mechanism of Protein Synthesis and its Regulation, p. 295. Amsterdam: North-Holland 1972.Google Scholar
  36. 36.
    Wittman, H. G.: personal communication.Google Scholar
  37. 37.
    Mizusiiima, S., Nomura, M.: Nature (Lond.) 226, 1214 (1970).CrossRefGoogle Scholar
  38. 38.
    Min Jou, W., Haegeman, G., Ysebaert, M., Fiers, W.: Nature (Lond.) 237, 82 (1972).CrossRefGoogle Scholar
  39. 39.
    Lodish, H.F.: J. molec. Biol. 50, 689 (1970).PubMedCrossRefGoogle Scholar
  40. 40.
    Kaempfer, R.: Proc. nat. Acad. Sci. (Wash.) 68, 2458 (1971).CrossRefGoogle Scholar
  41. 41.
    Kaempfer, R.: J. molec. Biol. 71, 583 (1972).PubMedCrossRefGoogle Scholar
  42. 42.
    Hershey, J. W. B., Dewey, K. F., Thach, R. E.: Nature (Lund.) 222, 944 (1969).CrossRefGoogle Scholar
  43. 43.
    Vermeer, C., De Kievit, R. J., Van Alphen, W. J., Bosch, L.: FEBS Letters 31, 273 (1973).PubMedCrossRefGoogle Scholar
  44. 44.
    Groner, Y., Revel, M.: Nature (Lend.) 219, 232 (1968).CrossRefGoogle Scholar
  45. 45.
    Guthrie, C., Nomura, M.: Nature (Lond.) 219, 232 (1968).CrossRefGoogle Scholar
  46. 46.
    Sabol, S., Ochoa, S.: Nature (Lund.) New Biol. 234, 233 (1971).Google Scholar
  47. 47.
    Salas, M., Hille, M. B., Last, J. A., Wahba, A. J., Ochoa, S.: Proc. nat. Acad. Sci. (Wash.) 57, 387 (1967).CrossRefGoogle Scholar
  48. 48.
    Rudland, P. S., Whybrow, W. A., Clark, B. F. C.: Nature (Lend.) New Biol. 231, 76 (1971).CrossRefGoogle Scholar
  49. 49.
    Lockwood, A. H., Ciiakraborty, P. R., Maitra, U.: Proc. nat. Acad. Sci. (Wash.) 68, 3122 (1971).CrossRefGoogle Scholar
  50. 50.
    Thach, S. S., Track, R. E.: Proc. nat. Acad. Sci. (Wash.) 68, 1791 (1971).CrossRefGoogle Scholar
  51. 51.
    Kaempfer, R., Kaufman, J.: Proc. nat. Acad. Sci. (Wash.) 70, 1222 (1973).CrossRefGoogle Scholar
  52. 52.
    Gesteland, R. F., Spahr, P. F.: Cold Spr. Harb. Symp. quant. Biol. 34, 717 (1969).CrossRefGoogle Scholar
  53. 53.
    Falvey, A.K., Staehelin, T.: J. molec. Biol. 53, 21 (1970).PubMedCrossRefGoogle Scholar
  54. 54.
    Lockwood, A.H., Sarhar, P., Maitra, L.I.: Proc. nat. Acad. Sci. (Wash.) 69, 3602 (1972).CrossRefGoogle Scholar
  55. 55.
    Hershey, J.: personal communication.Google Scholar
  56. 56.
    Van Dielten, G., Noll, M., Noll, H.: To be published.Google Scholar
  57. 57.
    Grünberg-Manago, M.: personal communication.Google Scholar
  58. 58.
    Kolakofsky, D., Dewey, K. F., Hershey, J. W. B., Thach, R. E.: Proc. nat. Acad. Sci. (Wash.) 61, 1066 (1968).CrossRefGoogle Scholar
  59. 59.
    Chae, Y.-B., Mazumder, R., Ochoa, S.: Proc. nat. Acad. Sci. (Wash.) 62, 1181 (1969).CrossRefGoogle Scholar
  60. 60.
    Lelong, J. C., Grunberg-Manago, M., Dondon, J., Gros, D., Gros, F.: Nature (Lond.) 226, 505 (1970).CrossRefGoogle Scholar
  61. 61.
    Highland, J. H., Bodley, J. W., Gordon, J., Hasenbank, R., Stöfler, G.: Proc. nat. Acad. Sci. (Wash.) 70, 147 (1973).CrossRefGoogle Scholar
  62. 62.
    Schreier, M. H., Staehelin, T.: Nature (Lond.) New Biol. 242, 35 (1973).Google Scholar
  63. 63.
    Darnbrough, C., Legon, S., Hunt, T., Jackson, R. J.: J. molec. Biol. 76, 379 (1973).PubMedCrossRefGoogle Scholar
  64. 64.
    Weber, H. J.: Molec. gen. Genet. 119, 233 (1972).PubMedCrossRefGoogle Scholar
  65. 65.
    Hafke, B., Noll, M., Noll, H.: Submitted for publication.Google Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1973

Authors and Affiliations

  • Hans Noll
    • 1
  • Markus Noll
    • 1
  • Bern Hapke
    • 1
  • Gerbrand van Dieijen
    • 1
  1. 1.Department of Biological SciencesNorthwestern UniversityEvanstonUSA

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