Abstract
Micromethods are of great use for the neurochemical analysis of samples of tissue from defined regions of the brain as they do not require material from a vast number of animals. Micromethods are also often the only methods for biochemical analysis when the quantity of material is limited. This article will outline the micromethods currently in use in our laboratory and will describe some results obtained by these procedures.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Althaus, H.-H., Briel, G., Dames, W., Neuhoff, V.: Zelluläre und molekulare Grundlagen der nervösen Erregungsspeicherung, Neurochemische Mikroanalysen des Rückenmarks der Katze nach post-tetanischer Potenzierung monosynaptischer Reflexe. In: Sonderforschungsbereich 33 Nervensystem und biologische Information, Gottingen 1969–1972, p. 107–171 (1972).
Ansorg, R., Dames, W., Neuhoff, V.: Arzneimittel-Forschung 21, 699–710 (1971).
Ansorg, R., Neuhoff, V.: Intern. J. Neuroscience 2, 151–160 (1971).
Brante, G.: Acta physic, scand. 18, Suppl. 63, 1–184 (1949).
Briel, G., Neuhoff, V.: Hoppe-Seylers Z. physiol. Chem. 353, 540–553 (1972).
Briel, G., Neuhoff, V., Osborne, N. N.: Intern. J. Neuroscience 2, 129–136 (1971).
Cleveland, D., Haase, J., Ross, H.-G., Vogel, B.: Zelluläre und molekulare Grundlagen der nervösen Erregungsspeicherung, Motoneurone, post-tetanische Potenzierung und recurrente Inhibition. In: Sonderforschungsbereich 33 Nervensystem und biologische Information, Göttingen 1969–1972, p. 96–106 (1972).
Cremer, Th., Dames, W., Neuhoff, V.: Hoppe Seylers Z. physiol. Chem. 353, 1317–1329 (1972).
Curtis, D. R.: Pore. Aust. Assoc. Neurologists 7, 55–60 (1970).
Curtis, D. R., Eccles, J. C.: J. Physiol. 150, 374–398 (1960).
Davies, B. J.: Ann. N.Y. Acad. Sci. 121, 404–427 (1964).
Davison, A. N., Kacmarek, L. K.: Nature 234, 107–108 (1971).
Eccles, J. C., McIntyer, A. K.: J. Physiol. 121, 492–516 (1953).
Edström, J.-E., Neuhoff, V.: Microelectrophoresis for RNA and DNA base analysis, in: Micromethods in Molecular Biology, ed. by V. Neuhoff, Berlin, Heidelberg, New York: Springer 1973.
Frey, W.: Freie Aminosäuren der Sehbahn des Kaninchens, Inauguraldissertation. Göttingen (1972).
Gray, W. R., Hartley, B. S.: Biochem. J. 89, 59 P, 379–380 (1963).
Grossbach, U.: Biochem. biophys. Acta 107, 180–182 (1965).
Hydén, H., Nature (Lond.) 184, 433–435 (1959).
Hydén, H., Bjurstam, K., McEwen, B.: Annal. Biochem. 17, 1–15 (1966).
Kandel, E. R., Spencer, W. A.: Physiol. Rev. 48, 65–134 (1968).
Kolin, A.: J. Chem. Phys. 22, 1628–1629 (1954).
Llyod, D. P. C.: J. gen. Physiol. 33, 147–170 (1949).
Maurer, H. R.: Disc electrophoresis and related techniques of polyacryamide gel electrophoresis. Working methods in modern science, ed. K. Fischbeck, Berlin, New York: Walter de Gruyter 1971.
McEwen, B., Hyden, H.: J. Neurochem. 13, 823–833 (1966).
ter Meulen, V., Muller, D., Enders-Ruckle, G., Neuhoff, V., Kackel, M. Y., Joppich, G.: Dtsch. Med. Wschr. 93, 1303–1308 (1968).
ter Meulen, V., Muller, D., Neuhoff, V., Joppich, G.: Acta Neurop. 15, 128–141 (1970).
Moore, B. W.: Biochem. biophys. Res. Commun. 19, 739–744 (1965).
Moore, B. W., Perez, V. J.: Specific acidic proteins of the nervous system. In: Physiol and Biochem. Aspects of nervous integration, ed.: Carlson, F. D., Prentic Hall, pp. 343–359 (1968).
Neuhoff, V.: Naturwissenschaften 54, 287–288 (1967).
Neuhoff, V.: Arzneimittel-Forsch. 18, 35–39 (1968).
Neuhoff, V.: Analyt. Biochem. 41, 270–271 (1971).
Neuhoff, V.: Micromethods in molecular biology. Berlin, Heidelberg, New York: Springer 1973.
Neuhoff, V., Briel, G., Maelicke, A.: Arzneimittel-Forsch. 21, 104–107 (1971).
Neuhoff, V., von der Haar, F., Schlimme, E., Weise, M.: Hoppe-Seylers Z. physiol. Chem. 350, 121–128 (1969).
Neuhoff, V., Müller, D., ter Meulen, V.: Z. wiss. Mikr. 69, 65–72 (1968).
Neuhoff, V., Weise, M.: Arzneimittel-Forsch. 20, 368–372 (1970).
Ornstein, L.: Ann. N. Y. Acad. Sci. 121, 321–349 (1964).
Osborne, N. N.: Intern. J. Neuroscience 3, 215–228 (1972a).
Osborne, N. N.: Brain Res. 41, 237–241 (1972b).
Osborne, N. N., Althaus, H.-N., Neuhoff, V.: Comp. Biochem. Physiol. 43 B, 671-679 (1972).
Osborne, N. N., Ansorg, R., Neuhoff, V.: Intern. J. Neuroscience 1, 259–264 (1971).
Osborne, N. N., Briel, G., Neuhoff, V.: Intern. J. Neuroscience 1, 265–272 (1971).
Osborne, N. N., Cottrell, G. A.: Experientia 28, 656–658 (1972).
Osborne, N. N., Neuhoff, V.: Naturwissensdiaften, in press.
Osborne, N. N., Powell, B., Cottrell, G. A.: Brain Res. 41, 379–386 (1972).
Pun, J. Y., Lombrozo, K.: Annal. Biochem. 9, 9–20 (1964).
Quentin, C.-D., Neuhoff, V.: Intern. J. Neuroscience 4, 17–24 (1972).
Roberts, E. Baxter, C. F., van Harreveld, A., Wiersma, C. A. G., Adey, W. R., Killam, K. F., teds. Inhibition in the nervous system and gamma-amino-butyric acid. Oxford, New York, London, Paris: Pergamon-Press 1960.
Rüchel, R.: Mikroelektrophoresen von RNS-Basen, Anwendung zur Untersuchung bestimmter Hirnregionen und kritische Analyse der Methode. Inauguraldissertation, Göttingen (1971).
Schiefer, H.-G., Neuhoff, V.: Hoppe-Seylers Z. physiol. Chem. 352, 913–926 (1971).
Seiler, N.: Methods of Biochemical Analysis, Use of the Dansyl reaction in Biochemical Analysis Vol. 18, 259–337, ed. by D. Glick. New York, London, Sidney, Toronto: Intersc. Publ. John Wiley-Sohn, 1970.
Svensson, H: Acta Chem. Scand. 15, 325–341 (1961).
Wilkinson, J. H: Isoenzymes. 2nd. ed. 1970. Chapman and Hall Ltd. London.
Woods, K. R., Wang, K. T.: Biochim. biophys. Acta 133, 369–370 (1967).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1974 Springer-Verlag Berlin · Heidelberg
About this chapter
Cite this chapter
Neuhoff, V. (1974). The Application of Micromethods to Neurochemistry. In: Genazzani, E., Herken, H. (eds) Central Nervous System. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65714-6_12
Download citation
DOI: https://doi.org/10.1007/978-3-642-65714-6_12
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-65716-0
Online ISBN: 978-3-642-65714-6
eBook Packages: Springer Book Archive