Plaque Assays and Current Concepts of Regulation in Arenavirus Infections
Use of recently developed plaque assays for LCM virus-infected cells and free virus has led to a better understanding of the in vitro host-virus interactions. Many LCM virus stocks, even at high input multiplicities of infection, have very low efficiencies of infecting cells. Under these conditions where multiple cycles of infection are necessary to achieve total infectivity of a BHK21/13 S monolayer there appears to be a preferential cell to cell transfer of the viral genome. The low efficiency of virus stocks in forming infective centers is due to an interference phenomenon that can be readily demonstrated using an L cell monolayer plaque assay. The properties of the interfering agent in recently infected cultures as well as in long-term infections are those of defective interfering virus. Further studies to elucidate the host-virus relationship in the regulation of virus production have shown that conditions favoring cell replication enhance virus replication or maturation.
KeywordsPlaque Assay Virus Stock Virus Yield Interference Phenomenon Free Virus
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