Summary
New antigens in LCM virus-infected L cells were studied by means of immunofluorescence and cytotoxicity procedures. With hyper immune antisera directed against all viral components and containing both neutralizing and complement-fixing antibodies, indirect immunofluorescent staining of fixed cells reveals cytoplasmic antigen which is distributed in a finely diffuse as well as a coarsely granular pattern. Employing both immunofluorescent staining and cytotoxicity assays, these sera also detect viral antigen on the surface of LCM virus-infected cells.
In contrast, only granular fluorescence and no surface antigen can be demonstrated when antisera specific for a complement-fixing antigen extracted from infected cultures are employed. Persistently infected L cells, whose cytoplasm contains masses of brightly fluorescing material, fail to display detectable surface antigen using either type of antisera.
It is concluded that at least 2 new antigens are produced in the interior of an LCM virus-infected L cell, one of which may also appear on its surface. The other antigen, which is produced in large quantities in infected cells and which is characterized by its complement-fixing potential, is not represented on the cell surface.
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Rutter, G., Gschwender, H.H. (1973). Antigenic Alteration of Cells in Vitro Infected with LCM Virus. In: Lehmann-Grube, F. (eds) Lymphocytic Choriomeningitis Virus and Other Arenaviruses. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65681-1_5
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DOI: https://doi.org/10.1007/978-3-642-65681-1_5
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